Tithonia diversifolia (Hemsl.) A. Gray has significant potential as a forage source in silvopastoral systems, particularly in tropical conditions. However, its intensity and frequency management differ from those commonly applied to grasses. This study aims to evaluate T. diversifolia under two harvest intensities (30 and 40 cm stubble height) and four harvest frequencies (21, 28, 35, and 42 days), aligning with Brazil’s traditional tropical grass management practices. Biomass production and nutritive value of forage, as well as in vitro rumen fermentation parameters were assessed. We observed that the tested harvest intensities have a limited impact on the biomass production and nutritional value of T. diversifolia. Despite the linear increase in biomass production, lower harvest frequencies (i.e., longer regrowth period) significantly affects nutritional value, impacting the products of ruminal fermentation, as indicated by the in vitro assay. The methane production per gram of degraded organic matter (OM) and the OM allocated for microbial biomass production are affected by the harvest frequencies. Additionally, we observed that rainfall and canopy height have stronger correlation with biomass production than harvest frequencies controlled by days. We conclude that T. diversifolia is minimally affected by harvest intensities and, for Brazilian edaphoclimatic conditions, it can be harvested with higher frequencies than those suggested for other regions.