Simaroubaceae family: botany, chemical composition and biological activities

Alves, Iasmine A.B.S.; Miranda, Henrique Marcelino; Soares, Luiz Alberto Lira; Randau, Karina Perrelli

doi:10.1016/j.bjp.2014.07.021

Cited by 92 publications

(59 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…18 Many species of the Simaroubaceae family display prominent anticancer activity. 19 The plant collected from (ICMR-NITM) Campus, was identified according to their taxonomical characters as Simarouba glauca and analysed for the presences of phytoconstituents with five solvent extracts. In the present study 100 g of S. glauca leaves powder gave total yield of 6.8 g in chloroform, 9.6 g in ethyl acetate, 24.64 g in methanol, 16.52 g in ethanol, 20.80 g in Aqueous and 31.2 g in hydroalcoholic solvent w/w respectively with reference to the air dried plant material.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Puranik¹,

Ghagane²,

Nerli³

et al. 2017

View full text Add to dashboard Cite

Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. Phytochemical investigation was done using biochemical tests. Total phenolic content was estimated by Folin-Ciocalteu reagent (FCR) method. Antioxidant potential of leaf extracts was analyzed by Ferric ion reducing antioxidant power (FRAP) assay, Phosphomolybdenum (PM) assay and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In vitro anticancer activity on T-24 bladder cancer cell line was assessed by MTT assay. Statistical analysis used: Statistical analysis of data was performed by analysis of variance (one-way ANOVA) and level of statistical significance between groups was carried out using GraphPad Prism version 5.0 for Windows (GraphPad Software, San Diego, CA, USA). Results: Phytochemical analysis revealed the presence of rich secondary metabolite present in all the solvent extracts. Hydroalcoholic extract showed highest presence of phenolic content (92.38±0.29 mg/g) GAE. Ethanol and methanol extract showed highest antioxidant capacity in DPPH, FRAP and PM assay as compared to the other extracts based on the test performed. The results confirmed that ethanol extract significantly (p<0.05) inhibited T-24 cell line with IC 50 value (533.55±25.02 µg/mL) as compared to standard drug doxorubicin (0.16µM/mL). Conclusions: The results of the present findings strengthen the potential property of Simarouba glauca as a resource for the discovery of novel antioxidant and anticancer agents.Key words: Antioxidant, Anticancer, Bladder Cancer, Phytochemical, Simarouba glauca. Key Messages: The use of medicinal plants have been practiced to treat cancer aliments across the world. Our study demonstrates the implication of herbal extracts to inhibit cancer cell lines at in vitro levels. However, the further approaches at in vivo level using lead compounds will give core insights of S. glauca extracts on Bladder cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Puranik¹,

Ghagane²,

Nerli³

et al. 2017

View full text Add to dashboard Cite

show abstract

“…[11] However, canthin-6-one alkaloids with fluorescent properties also occur in Simaba. [11][12] Although Simaba is the largest genus in the family, the chemical composition and biological activity of most of the species of this genus have not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Canthin‐6‐one Alkaloids from Simaba bahiensis: Isolation, Identification, and Cell‐Labeling Properties

et al. 2019

View full text Add to dashboard Cite

Canthin‐6‐one alkaloids, which are present in plants of the genus Simaba, are natural compounds that are capable of acting as fluorescent probes. However, the chemical composition and fluorescent properties of most species of this genus have not been analyzed. The objective of this study was to characterize the fluorescent properties of an extract of S. bahiensis and identify the chemical entities responsible for these properties. In addition, the cell‐labeling properties of the fluorescent dye from A and of the isolated compounds were characterized by confocal fluorescence microscopy and flow cytometry. One quassinoid and three fluorescent alkaloids were isolated from S. bahiensis, all compounds were identified by using NMR spectroscopy and high‐resolution mass spectrometry. Staining experiments and HPLC‐FL analysis shown that canthin‐6‐one alkaloids are the main green fluorescent compounds in the analyzed dyes. All compounds evaluated showed a cytoplasmic marker with a residence time of 24 h. The present study is the first to describe the presence of canthin‐6‐one alkaloids in S. bahiensis, in addition to demonstrating promising cell‐labeling properties of fluorescent compounds from S. bahiensis with broad emission wavelengths.

show abstract

“…Canthi-6-one alkaloids occur in plants from families Rutaceae, Simaroubareace, Amaranthaceae, Caryophyllaceae and Zygophylaceae [1][2][3]. More than 60 members of this type of alkaloid were isolated from natural sources since first report in 1952 [4].…”

Section: Introductionmentioning

confidence: 99%

“…These alkaloids have shown a wide range of pharmacological properties including cytotoxic, antibacterial, antifungal, antiparasict, antiviral, antiinflammatory and beside it, some of them show interesting photophysical properties that gives an interesting use the fluorescent dye probe in florescent cellular microscopy. New studies have been carring out in an attempt to identify new alkaloids with pharmacological properties by synthesis or isolation from different natural sources [2]. In family Simaroubaceae more than 30 canthi-6-one alkaloids have already been described with different substitution patterns and it shows that the investigation of new alkaloids in this family it's an important way to search new chemical entities [2,3].…”

mentioning

confidence: 99%

“…New studies have been carring out in an attempt to identify new alkaloids with pharmacological properties by synthesis or isolation from different natural sources [2]. In family Simaroubaceae more than 30 canthi-6-one alkaloids have already been described with different substitution patterns and it shows that the investigation of new alkaloids in this family it's an important way to search new chemical entities [2,3]. In this work we describe the first phytochemical study of Simaba bahiensis (Simaroubaceae), collected at the city of Camaçari, Bahia State, Brazil.…”

mentioning

confidence: 99%

See 1 more Smart Citation

NMR analysis of a new canthin-6-one alkaloid from Simaba bahiensis (Simaroubaceae)

Costa

Fontes

Abreu

et al. 2017

Proceedings of MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd Edition

View full text Add to dashboard Cite

Graphical Abstract Abstract.Alkaloids of the canthi-6-one type have been reported in differents natural sources. These alkaloids have showed a wide range of pharmacological properties including cytotoxic, antibacterial, antifungal, antiparasict, antiviral, anti-inflammatory and beside it, some of them show excellent photophysical properties that gives an interesting use as fluorescent dye probe in florescent cellular microscopy. Because it, new studies have been carrying out in an attempt to identify new alkaloids with pharmacological properties. Some researchers have been trying to synthesize new derivatives or identify new compounds in natural sources. Among the >60 canthin-6-one alkaloids already reported in natural sources, more the half are present in plants of the family Simaroubaceae. In the Simaba genus, up to the present, only eighteen alkaloids was described in seven species, but other plants of the genus that occur in the brazilian's flora haven't been studied yet. In this work we describe the first phytochemical study of the specie Simaba bahiensis (Simaroubaceae), collected at the city of Camaçari, Bahia State, Brazil. Also, it's related complete structural determination using differents NMR experiments and HRMS of a new canthin-6-one alkaloid in addition to two others already known MOL2NET, 2017, 3, doi:10.3390/mol2net-03-xxxx IntroductionCanthi-6-one alkaloids occur in plants from families Rutaceae, Simaroubareace, Amaranthaceae, Caryophyllaceae and Zygophylaceae [1][2][3]. More than 60 members of this type of alkaloid were isolated from natural sources since first report in 1952 [4]. These alkaloids have shown a wide range of pharmacological properties including cytotoxic, antibacterial, antifungal, antiparasict, antiviral, antiinflammatory and beside it, some of them show interesting photophysical properties that gives an interesting use the fluorescent dye probe in florescent cellular microscopy. New studies have been carring out in an attempt to identify new alkaloids with pharmacological properties by synthesis or isolation from different natural sources [2]. In family Simaroubaceae more than 30 canthi-6-one alkaloids have already been described with different substitution patterns and it shows that the investigation of new alkaloids in this family it's an important way to search new chemical entities [2,3]. In this work we describe the first phytochemical study of Simaba bahiensis (Simaroubaceae), collected at the city of Camaçari, Bahia State, Brazil. Also, it's related complete structural determination using differents NMR experiments and HRMS of a new canthin-6-one alkaloid and two others already known.

show abstract

Simaroubaceae family: botany, chemical composition and biological activities

Cited by 92 publications

References 145 publications

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Fluorescent Canthin‐6‐one Alkaloids from Simaba bahiensis: Isolation, Identification, and Cell‐Labeling Properties

NMR analysis of a new canthin-6-one alkaloid from Simaba bahiensis (Simaroubaceae)

Contact Info

Product

Resources

About