Nowadays, different structural members can be used in structures. The mechanical properties of the materials are variable. For this reason, it is very important to identify the material properties correctly. The main parameter used to define the material properties is the elasticity modulus. In this study, the elasticity modulus of structural members was determined by Model Calibration Method using dynamic characteristics obtained from Ambient Vibration Test data. The application of the proposed method was presented on a steel structure member. First of all, experimental natural frequencies were obtained by conducting Environmental Vibration Test on the selected model. A measurement system consisting of accelerometers for experimental measurements and an electronic circuit used as signal collection unit are designed. The data obtained from this measurement system were transferred to the computer environment and analyzed in the Matlab program. Using the SAP2000 package program, the theoretical natural frequencies were found by Finite Element Method. The difference between the experimental and theoretical results was reduced to the lowest as a result of repeated analysis by modifying the elasticity modulus in the Finite Element model created in SAP2000 program via software generated in Matlab. The calibrated finite element model and the elasticity modulus of the steel structural member are determined by the process.