Simian virus 40-Chinese hamster kidney cell interaction II. The semipermissivity of the cell system

Lavialle, Christian; Suárez, H. G.; Morris, A. G.; Estrade, S.; Stévenet, J.; Cassingéna, R.

doi:10.1007/bf01318008

Cited by 10 publications

(11 citation statements)

References 16 publications

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“…We have further shown that Chinese hamster kidney cells, whether transformed or not by SV40, were all potentially permissive for the virus (12,13).…”

Section: Introduetionmentioning

confidence: 97%

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Suárez

Lavialle

Estrade

et al. 1978

Archives of Virology

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A somatic cell hybrid (Cl. 6d) was originated from the fusion of mouse 3T3-4E) and spontaneous yielder SV 40-transformed Chinese hamster (CHK/SVLP AG) cells. During the early stages of its history, the C1. 6d hybrid underwent a rapid chromosome loss, preferentially loosing hamster chromosomes. This was not a constant tendency of the hybrid cells. As the parental CHK)SVLP AG cells, the hybrid cells were always found 100 per cent SV40 T-antigen positive. While CHK/SVLP AG cells infectious SV 40 DNA, V-antigen and virus were regularly detected, in the hybrid cells only infectious DNA was occasionally detected. This was not due either to the loww of an essential Chinese hamster gene(s) or to the presence of an inhibiting mouse cell component(s); it was apparently the consequence of inability of the cells to properly activate the resident SV 40 genome(s). After superinfection with SV 40 DNA, the hybrid cells-though capable of synthesizing SV 40 V-antigen--were unable to ensure virus assembly. Experimental evidence was obtained suggesting that SV 40 maturation is dependent of a cellular function(s).

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“…We have further shown that Chinese hamster kidney cells, whether transformed or not by SV40, were all potentially permissive for the virus (12,13).…”

Section: Introduetionmentioning

confidence: 97%

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Suárez

Lavialle

Estrade

et al. 1978

Archives of Virology

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“…Upon infection with SV4o virus or virus DNA, Chinese hamster kidney cells supported virus DNA and virus synthesis at a low level and this cell system was defined as semipermissive (Lavialle et al 1976). To demonstrate infectious SV4o virus synthesis, these cells had to be infected with virus DNA in order to overcome their limited susceptibility to the virus particles and to avoid masking of the virus progeny by the input virus inoculum (Lavialle et aL I976).…”

Section: Stimulation Of S V4o Production By MC Treatment Of Chk Cl Imentioning

confidence: 99%

Simian Virus 40 -- Chinese Hamster Kidney Cell Interaction. IV. Enhanced Virus Replication in Infected Cells upon Treatment with Mitomycin C

Lavialle

Morris

Suárez

et al. 1977

Journal of General Virology

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SUMMARYChinese hamster kidney cells are semi-permissive to simian virus 40 (SV4o). Exposure to mitomycin C (MC) of Chinese hamster kidney cells infected with SV4o DNA enhanced the yield of infectious virus Io-to zoo-fold. This stimulation occurred whether the treatment was performed before or after infection. A simultaneous increase in the number of V antigen-synthesizing cells and virus-producing cells, as well as the virus burst size, was observed upon MC pretreatment, whereas the proportion of T antigen-synthesizing cells remained unchanged. MC pretreatment clearly stimulated virus DNA replication in SV4o virus-infected cells. Cells treated with MC exhibited an unbalanced growth pattern, with continuing protein synthesis in the absence of cell division and a markedly reduced ability to replicate the cellular DNA. These results suggest that MC enhances the permissiveness of Chinese hamster kidney cells by inducing the synthesis of a specific cellular factor(s) required for SV4o replication in these cells. Exposure to ultraviolet light also enhanced infectious virus production in Chinese hamster kidney cells.

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“…There was no significant difference between these clones, at least in terms of the total number of Chinese hamster chromosomes identified. These three superhybrid clones were also capable of synthesizing SV40 V-antigen and virus after superinfection [15], Knowing that the Chinese hamster kidney cells, whether transformed or not by SV40, were all potential ly permissive for the virus [11] we thought it was im portant to try and see whether or not certain chromo somal pairs were involved in the expression of viral functions. Therefore, we compared each pair of Chinese hamster chromosomes present in Cl 6d with its evolu tion in Cl 6d.6BU.…”

Section: Comparison Of Mouse Chromosomes (Fig 5 Table Vi)mentioning

confidence: 99%

Chromosomes Involved in Production of Infectious SV40 Particles in Mouse/SV40-Transformed Chinese Hamster Cell Hybrids

Berebbi¹,

Simonetti²,

Lepoint³

et al. 1981

Oncology

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The clone 6d hybrid, capable of expressing the virus-specific T-antigen but unable to produce infectious virus particles after superinfection, presented a complete mouse (3T3–4E) chromosome complement and a significant loss of Chinese hamster (CHK/SVLP AG) chromosomes. Similar properties were displayed by a BUdR-resistant derivative of the CI 6d hybrid (CI 6d.6BU). Three independent superhybrid clones (CL 10B, CI 10C, CI 11A) isolated after backcross of the CI 6d.6BU hybrid with a nontransformed Chinese hamster kidney cell line (CHK/AG) were able to produce infectious SV40 virus. In spite of the loss of mouse chromosomes, there was no significant difference in the average number of chromosomes between the CI 6d.6BU and the superhybrid clones. Thus, the Chinese hamster chromosomes seemed to compensate for the loss of the mouse chromosomes. Although the effect of Chinese hamster chromosomes cannot be totally disregarded, our data suggested a positive correlation between the inability to produce infectious SV40 and the presence of certain mouse chromosomes.

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Simian virus 40-Chinese hamster kidney cell interaction II. The semipermissivity of the cell system

Cited by 10 publications

References 16 publications

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Simian Virus 40 -- Chinese Hamster Kidney Cell Interaction. IV. Enhanced Virus Replication in Infected Cells upon Treatment with Mitomycin C

Chromosomes Involved in Production of Infectious SV40 Particles in Mouse/SV40-Transformed Chinese Hamster Cell Hybrids

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