Similar inductive effects of enamel and dentin matrix derivatives on osteoblast-like cell response over SLA titanium surface

Ramenzoni, Liza L.; Hirsiger, Constanze; Weber, Franz E.; Attin, Thomas; Schmidlin, P. R.

doi:10.1016/j.archoralbio.2019.104552

Cited by 6 publications

(8 citation statements)

References 29 publications

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“…One limitation of the study might be a relatively low number of independent experiments. We repeated cell viability/proliferation experiments three times, which agrees with previous studies using a similar design (e.g., [24,46] who performed three repetitions). PCR and ELISA experiments were repeated four and five times, respectively, because these experiments showed more considerable variability.…”

Section: Discussionsupporting

confidence: 77%

Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response

Wehner

Laky

Shokoohi-Tabrizi

et al. 2021

J Mater Sci: Mater Med

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The aim of this in vitro study was to evaluate the effects of erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation on titanium surface topography and the proliferation and differentiation of osteoblasts using standard clinical treatment settings. Er:YAG laser irradiation at two levels ((1): 160 mJ, pulse at 20 Hz; (2): 80 mJ, pulse at 20 Hz) was applied to moderately rough and smooth titanium disks before MG-63 osteoblast-like cells were cultured on these surfaces. Titanium surface and cell morphology were observed by scanning electron microscopy. Cell proliferation/viability was measured by CCK-8 test. Gene expression of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL), and collagen type 1 was measured by qPCR, and OPG and OC protein production was determined by enzyme-linked immunosorbent assay. Treatment with Er:YAG laser at 160 mJ/20 Hz markedly caused heat-induced fusion of titanium and cell condensation on moderately rough surfaces, but not in smooth surfaces. MG-63 proliferation/viability decreased after 5 days in moderately rough surfaces. The expression of ALP, OC, OPG, and collagen type 1 was unaffected by laser treatment at 160 mJ/20. Laser irradiation at 80 mJ/20 Hz enhanced RANKL gene expression after 5 days in moderately rough surfaces. Study results suggest that Er:YAG laser irradiation at clinically relevant setting has no essential effect on osteogenic gene and protein expression of osteoblasts. However, surface structure, cell attachment, and proliferation are influenced by both treatment protocols, which implies that caution should be taken in the clinical treatment of peri-implant diseases when Er:YAG laser is used.

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Section: Discussionsupporting

confidence: 77%

Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response

Wehner

Laky

Shokoohi-Tabrizi

et al. 2021

J Mater Sci: Mater Med

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“…Seven in vitro studies observed the behavior of enamel matrix derivatives on titanium surface, evaluating the ability of inducing and promoting osteoprogenitor cells [ 13 , 14 , 15 , 16 , 17 ], primary gingival fibroblasts [ 18 ] or endothelial cells [ 19 ] ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…More recently, the study by Ramenzoni et al published in 2020, aimed at evaluating the response of MC3T3 osteoblast-like cells cultivated on SLA titanium disks with EMD or dentin matrix derivative (DMD) or left uncoated [ 17 ]. Interestingly, EMD and DMD acted similarly in the experimental conditions, both increasing the osteoblasts proliferation and activity.…”

Section: Resultsmentioning

confidence: 99%

“…Seven in vitro studies observed the behavior of enamel matrix derivatives on titanium surface, evaluating the ability of inducing and promoting osteoprogenitor cells [13][14][15][16][17], primary gingival fibroblasts [18] or endothelial cells [19] (Table 1). The study by Schwarz et al published in 2004 investigated the effects of EMD on human osteoblasts-like cells attachment, viability, and proliferation on titanium implants with sand-blasted acid-etched surfaces (SLA), evaluating the samples for 6 days [13].…”

Section: In Vitro Studiesmentioning

confidence: 99%

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The Applications of Enamel Matrix Derivative in Implant Dentistry: A Narrative Review

et al. 2021

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Enamel matrix derivative (EMD) has been successfully used for periodontal regeneration in intrabony defects. Recently, its use for peri-implant bone regeneration has also been hypothesized. The aim of this paper is to review preclinical and clinical studies investigating the use of EMD in correspondence with titanium implants, alone or as an adjunct to other biomaterials. Clinical trials and case series with more than five cases were included. Seven in vitro studies evaluated the effect of EMD, placed on titanium surfaces: An increase in proliferation and viability of osteoblasts was observed in all but two studies. An increase in TGF-β1 and osteocalcin production, alkaline phosphatase activity, and angiogenesis was also reported. Nine animal studies investigated the use of EMD at implant placement or for bone regeneration of peri-implant bone defects, and some of them reported a significant increase in bone formation or bone-to-implant contact. In four of eleven clinical trials on humans, EMD was successfully used at implant placement. The other seven evaluated the use of EMD in protocols for the treatment of peri-implantitis. In conclusion, the results of EMD seem promising, but further randomized clinical trials are needed to evaluate its efficacy.

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“…Such biological elements are signaling factors that regulate cell development and growth, and they modulate extracellular matrix formation and also cell proliferation and migration [ 15 ]. The incorporation of growth factors in ARP modalities may therefore conceptually provide the opportunity to accelerate new bone formation and, consequently, ridge preservation [ 16 ]. A high quality systematic review and meta-analysis compared the overall effect of nine different ARP treatment modalities with tooth extraction alone.…”

Section: Introductionmentioning

confidence: 99%

Enamel Matrix Derivatives as an Adjunct to Alveolar Ridge Preservation—A Systematic Review

2023

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Purpose: To systematically assess the current evidence regarding the adjunctive application of enamel matrix derivatives (EMDs) during alveolar ridge preservation (ARP) following tooth extraction. Methods: A comprehensive literature search was conducted in MEDLINE, Cochrane Library, PsycINFO, Web of Science, Google Scholar, and Scopus to identify relevant randomized controlled clinical trials (RCTs). The primary outcome parameters of this systematic review were histomorphometric and radiographic data; secondary outcomes were the feasibility of implant placement after ARP as well as patient-related outcomes such as postoperative discomfort. Results: The search identified 436 eligible articles published from 2011 to 2022, but only five were ultimately included for data extraction (146 patients). Given the substantial heterogeneity among the included studies, no meta-analysis could be performed. The authors’ qualitative analysis showed marginally improved outcomes regarding an increased percentage of new bone formation after tooth extraction and a reduction in postoperative discomfort. Conclusions: Given the potential value of EMDs in other fields of regenerative dentistry, more consideration should be given to EMDs as an adjunctive treatment option in ARP. However, more well-controlled randomized clinical trials are necessary to evaluate the exact potential and impacts of EMDs.

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Similar inductive effects of enamel and dentin matrix derivatives on osteoblast-like cell response over SLA titanium surface

Cited by 6 publications

References 29 publications

Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response

Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response

The Applications of Enamel Matrix Derivative in Implant Dentistry: A Narrative Review

Enamel Matrix Derivatives as an Adjunct to Alveolar Ridge Preservation—A Systematic Review

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