Similar polypeptide composition of fast‐transported proteins in rat motor and sensory axons

Ma, Bisby

doi:10.1002/neu.480080403

Cited by 24 publications

(29 citation statements)

References 26 publications

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“…The finding of qualitative similarities in FTP synthesized by either retinal ganglion or dorsal root ganglion cells found in this study is in close agreement with previous work showing striking similarities in the FTP found in peripheral motor and sensory axons (Barker, Neale, and Gainer, 1976;Bisby, 1977;White and White, 1977) even when highly sensitive two dimensional gel electrophoretic techniques were used (Stone and Wilson, 1979). This suggests that the major FTP play a common role in the normal maintenance of axons and are not specific to the particular physiological function of that neuron (i.e.…”

Section: Discussionsupporting

confidence: 93%

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Redshaw¹,

Bisby²

1984

J. Neurobiol.

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After axotomy, changes in the composition of fast axonally transported proteins ( FTP ) within the peripheral nervous system (PNS) axons have been reported. The most significant and reproducible changes involved polypeptides found within the molecular weight range of 31.0 to 14.5 kilodaltons ( Bisby , 1980). We wished to determine whether similar changes following axotomy occur in axons of the central nervous system (CNS). Intracranial axotomy of the left optic tract was performed stereotaxically in rats. Six days post axotomy 50 muCi 35[S]-methionine was injected into the vitreous body of both eyes. FTP were isolated within the optic nerves 2 h after isotope injection. The nerve segments were processed for SDS-PAGE, fluorography, and compared to similarly prepared fluorographs of normal and eight day post-axotomy sciatic nerve segments. The labelling of 5 major polypeptide bands (S1, MW congruent to 28,000; S2a , MW congruent to 25,000; S2b , MW congruent to 23,000; T1, MW congruent to 20,200; and T2, MW congruent to 17,000) was studied by laser densitometry. Band S2b showed a highly significant (p less than 0.001) increase in concentration, while bands S1 and T1 demonstrated highly significant decreases in concentration following axotomy of the sciatic nerve. In contrast, after axotomy of the retinal ganglion cell axons the only significant change was a decrease (p less than 0.05) in T1. We suggest that failure of CNS axons to respond similarly to PNS axons following axotomy may be related to the failure of CNS axons to regenerate.

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Section: Discussionsupporting

confidence: 93%

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Redshaw¹,

Bisby²

1984

J. Neurobiol.

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“…In the present studies, neuronally derived axonal membrane proteins and lipids also do not remain together. Whereas the bulk of rapidly transported proteins travel in a characteristic wave over long distances (Ochs, 1972;Griffin, 1976;Bisby, 1977;Grafstein and Forman, 1980), only a small proportion of transported phospholipids travel in this fashion (Grafstein et al, 1975;Currie et al, 1978;Toews et al, 1979). This quantitative difference, interpreted as being a result of a prolonged release of phospholipid from the cell body (Grafstein et al, 1975;Currie et al 1978;Toews et al, 1979), suggests differences in mechanisms which take newly formed lipids and proteins from their site of synthesis to the sites where transport is initiated (Holtzmann and Mercurio, 1980).…”

Section: Discussionmentioning

confidence: 99%

Axonal Transport of Choline Lipids in Normal and Regenerating Rat Sciatic Nerve

Gould

Spivack

Sinatra

et al. 1982

Journal of Neurochemistry

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Biochemical methods were used to study the time course of transport of choline phospholipids (labeled by the injection of [3H]choline into the ventral horn of the lumbar spinal cord) in rat sciatic nerve. Autoradiographic methods were used to localize the transported lipid within motor axons. Transported phospholipid, primarily phosphatidylcholine, present in the nerve at 6 h, continued to accumulate over the following 12 days. No discrete waves of transported lipid were observed (a small wave of radioactive phospholipid moving at the high rate would have been missed); the amounts of radioactive lipid increased uniformly along the entire sciatic nerve. In light‐microscope autoradiographs, a class of large‐caliber axons, presumably motor axons, retained the labeled lipid. Some lipid, even at 6 h, was seen within the myelin sheaths. Later, the labeling of the myelin relative to axon increased. The continued accumulation of choline phospholipids in the axons probably signifies their prolonged release from cell bodies and their retention in various axonal membranes, including the axolemma. The build‐up of these phospholipids in myelin probably represents their transfer from the axons to the myelin sheaths surrounding them. When nerves are crushed and allowed to regenerate for 6 or 12 days, choline phospholipids transported during these times enter the regenerating nerve. In light and electron microscope autoradiographs, transported lipid was seen to be localized primarily in the regenerating axons. However, grains overlay the adjacent Schwann cell cytoplasm, indicating transported lipids were transferred from the regenerating axons to the associated Schwann cells. In addition, some cells not associated with growing axons were labeled, suggesting that phosphatidylcholine and possibly acetylcholine, carried to the regenerating axons by axonal transport, were actively metabolized in the terminal, with released choline label being used by other cells. These results demonstrate that axonal transport supplies mature and growing axons and their glial cells with choline phospholipids.

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“…The absence of major differences corresponding to tract-specific transported proteins has also been reported for the bifurcating neurons of the dorsal root ganglion (Barker et al, 1976;1977;Bisby, 1977;White and White, 1977;Stone et al, 1978) and for retinal ganglion cells which bifurcate to the lateral geniculate and superior colliculus (Siege1 and McClure, 1976;Wagner et al, 1979). However, in each bifurcated neuron, the transmitter of the nerve endings is probably the same.…”

Section: Comparison Of 35s-labeied Proteinsmentioning

confidence: 75%

“…Axonal transport of proteins has been studied extensively in the long, well-defined nerves of the peripheral nervous system (PNS) (Barker et al, 1976;1977;Bisby, 1977;White and White, 1977;Theiler and McClure, 1977;1978;Stone et al, 1978). In the central nervous system (CNS), the optic pathway is also well suited anatomically for such investigations, and the protein classes trans-ported to the optic relay nuclei after injection of radioactive precursor into the vitreous chamber of the eye have been defined in terms of molecular weight distributions and transport rates (Karlsson and Sjostrand, 1971;Marko et al, 1971;Willard et al, 1974;Siege1 and McClure, 1975;Willard and Hulebak, 1977;Wagner et al, 1979).…”

mentioning

confidence: 99%

“…In the central nervous system (CNS), the optic pathway is also well suited anatomically for such investigations, and the protein classes trans-ported to the optic relay nuclei after injection of radioactive precursor into the vitreous chamber of the eye have been defined in terms of molecular weight distributions and transport rates (Karlsson and Sjostrand, 1971;Marko et al, 1971;Willard et al, 1974;Siege1 and McClure, 1975;Willard and Hulebak, 1977;Wagner et al, 1979). Characterization of proteins transported in other CNS tracts in-cludes studies of olfactory tract (Weiss et al, 1978) and the central projection of the dorsal root ganglion (Barker et al, 1976;1977;White and White, 1977;Stone and Wilson, 1979).…”

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confidence: 99%

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Axonal Transport of [³⁵S]Methionine‐Labeled Proteins in Two Intra‐Brain Tracts of the Rat

Padilla

Morell

1980

Journal of Neurochemistry

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[35S]Methionine was stereotaxically injected into the dorsalateral geniculate body (DLGB) of adult male rats, and 1 h to 10 days post‐injection the DLGB and projection site (striate cortex) were dissected out and solubilized in 1% sodium dodecyl sulfate. Samples were analyzed for acid‐precipitable radioactivity, and radioactivity in different molecular weight classes was determined following discontinuous gel electrophoresis on both tube and slab gels. Acid‐precipitable radioactivity in the DLGB peaked by 4 h and then declined over the time period studied. The molecular weight distribution pattern was complex and did not change appreciably with time. Radioactivity in the striate cortex arrived in at least three waves: rapidly transported proteins arrived between 2 and 4 h; a second wave of transport began to arrive at about 7 h post‐injection and there was a slight rise in specific activity for 2 days; finally, at 3 days post‐injection, there was a steep increase with the arrival of the bulk of the transported material. The electrophoretic distribution pattern of proteins arriving in the first wave included 40–50 identifiable bands ranging in molecular weight from 13,000 to 200,000. Of particular interest was a radioactive band of apparent molecular weight of 110,000, which was prominent at 4 h, but by 12 h showed very little labeling. The second wave of radioactivity contained primarily proteins of molecular weight classes already present, although there were quantitative differences. Several proteins in the molecular weight range of 43,000 to 78,000 were identifiable as characteristic of the third wave of transported material. Results from a study following injection of a hippocampus were similar: the electrophoretic distribution pattern of radioactive proteins extracted from the injected hippocampus resembled that of the DLGB, and also did not vary appreciably with time, while radioactive proteins in the contralateral hippocampus had an electrophoretic distribution pattern similar to that of the striate cortex and changed with time in a similar manner.

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Similar polypeptide composition of fast‐transported proteins in rat motor and sensory axons

Cited by 24 publications

References 26 publications

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Axonal Transport of Choline Lipids in Normal and Regenerating Rat Sciatic Nerve

Axonal Transport of [³⁵S]Methionine‐Labeled Proteins in Two Intra‐Brain Tracts of the Rat

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Similar polypeptide composition of fast‐transported proteins in rat motor and sensory axons

Cited by 24 publications

References 26 publications

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Fast axonal transport in central nervous system and peripheral nervous system axons following axotomy

Axonal Transport of Choline Lipids in Normal and Regenerating Rat Sciatic Nerve

Axonal Transport of [35S]Methionine‐Labeled Proteins in Two Intra‐Brain Tracts of the Rat

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Axonal Transport of [³⁵S]Methionine‐Labeled Proteins in Two Intra‐Brain Tracts of the Rat