Similar protective effect of ischaemic and ozone oxidative preconditionings in liver ischaemia/reperfusion injury

Ajamieh, Hussam; Merino, Nelsón; Candelario‐Jalil, Eduardo; Menéndez, Silvia; Martı́nez-Sánchez, Gregorio; Re, Lamberto; Giuliani, Attilia; León, Olga Sonia

doi:10.1006/phrs.2002.0952

Cited by 85 publications

(91 citation statements)

References 32 publications

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“…It is generally accepted that the cirrhotic liver is particularly sensitive to ischemia. Although warm ischemia for cirrhotic liver should not exceed 30 min, prolonged HIO might cause dysfunction of the remnant liver, which is a major risk factor associated with postoperative morbidity and mortality 3 . Various methods such as the use of drugs, hypothermia, and ischemic preconditioning (IPC) have been used in order to protect the liver from injury induced by ischemia/reperfusion (I/R).…”

Section: Introductionmentioning

confidence: 99%

Effect of liver ischemic preconditioning in cirrhotic rats submitted to hepatic ischemia/reperfusion injury

Pacheco¹,

Gomes²,

Rodrigues³

et al. 2006

Acta Cir. Bras.

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Purpose:The main aim of this study was to determine the influence of ischemic preconditioning (IPC) on rat liver cirrhosis. Methods: Cirrhosis was induced in Wistar rats by occlusion of the hepatic duct. The animals were divided into four groups of six animals each: non-cirrhotic group (simulated operation only), cirrhotic control group (simulated operation in cirrhotic rats), I/R group (40-minute ischemia without IPC), and IPC group (cirrhotic rats with ischemia, previously submitted to IPC). The IPC procedure consisted of partial hepatic ischemia for five minutes, followed by 10 minutes of reperfusion. In the case of the IPC group, the animals were submitted to liver ischemia for 40 minutes after the preconditioning procedure, followed by 2 hours of reperfusion. Blood samples were collected for measurement of serum aminotransferases (ALT and AST). The respiratory control ratio (RCR), the mitochondrial membrane potential (MMP), and malondialdehyde (MDA) values in the hepatic tissue were analyzed. Nonparametric statistical analysis was used and a value of p<0.05 was considered statistically significant. Results: Ischemia did not induce significant increase in ALT and AST levels. MDA values were significantly higher in cirrhotic animals. MMP did not significantly change in cirrhosis and liver ischemia. Mitochondrial RCR decreased in liver cirrhosis, accentuated upon liver ischemia, and did not significantly change with IPC. Conclusion: Ischemic preconditioning does not protect the liver from hepatic injury induced by the ischemia/ reperfusion process. Key words: Liver Cirrhosis. Hepatic Ischemia. Ischemic Preconditioning. RESUMOObjetivo: O objetivo deste estudo foi determinar a influência do pré-condicionamento isquêmico (IPC) em fígados de ratos cirróticos. Métodos: A cirrose hepática foi induzida em ratos Wistar machos (250 a 300g) por oclusão, durante 30 dias, do ducto hepático comum.A seguir, os animais cirróticos foram divididos em três grupos de seis; Grupo controle cirrótico (operação simulada para isquemia/reperfusão/pré-condicionamento), Grupo I/R, isquemia de 40 minutos sem pré-condicionamento (IPC) e grupo IPC com isquemia precedida por IPC. O IPC consistiu de uma isquemia parcial por cinco minutos, seguida por 10 minutos de reperfusão. No grupo IPC, após o pré-condicionamento, os animais foram submetidos à isquemia hepática de 40 minutos seguida de 2 horas de reperfusão. Foram colhidas amostras de sangue para dosagem sérica de aminotransferases (ALT e AST). Razão de controle respiratório (RCR), potencial de membrana das mitocôndrias (PMM) e dosagem de malondialdeído (MDA) foram analisadas no tecido hepático. Analise estatística não paramétrica foi utilizada com nível de significância de 5% para as comparações entre grupos.Resultados: A isquemia não induziu aumento significativo das aminotransferases. Houve aumento significativo de MDA nos animais cirróticos.O PMM não se alterou significativamente na cirrose e na isquemia hepática. A RCR mitocondrial diminuiu na cirrose hepática e acentuou-se ...

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Section: Introductionmentioning

confidence: 99%

Effect of liver ischemic preconditioning in cirrhotic rats submitted to hepatic ischemia/reperfusion injury

Pacheco¹,

Gomes²,

Rodrigues³

et al. 2006

Acta Cir. Bras.

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“…La inhalación de oxigeno + ozono por 10 o 15 días produce pre-acondicionamiento al estrés oxidativo por ozono y protege al hígado del daño por isquemia y reperfusión, que se ha explicado por aumento de la expresión de enzimas antioxidantes (superóxido dismutasa, catalasa y glutatión peroxidasa), que probablemente gatillen el pre-acondicionamiento oxidativo por ozono [36][37][38] . Por otra parte, en simulaciones de shock endotóxico, el ozono protege a la rata disminuyendo TNF-α sanguíneo, esto se explicaría porque los antioxidantes generados por el ozono, administrado antes de la lesión, disminuyen el NF-kB y las citoquinas circulantes 39,40 .…”

Section: Discussionunclassified

Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

et al. 2017

Rev. méd. Chile

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Effect of copper sulphate on the lung damage induced by chronic intermittent exposure to ozone count, proteins, GGT and AP in BALF (p < 0.05). Foamy and pigmented alveolar macrophages were detected in all lungs of these animals (p < 0.001). Conclusions: Intratracheal instillation of a single dose of CuSO 4 in rats previously subjected to a chronic and intermittent exposure to ozone induces a neutrophil pulmonary inflammatory response and cytoplasmic damage in macrophages. (Rev Med Chile 2017; 145: 9-16)

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“…Ozone, when given in lower doses, exerts beneficial effects acting on the oxidant mechanisms (Ajamieh et al, 2002;Chen et al, 2008). In spite of encouraging results obtained with ozone therapy, its clinical use remains controversial due to the scarce knowledge of the mechanisms underlying its therapeutic action and the efficacy in heterogeneous diseases (Ajamieh et al, 2002;Gornicki & Gutsze, 2000).…”

Section: Ozone: Its General Effects and Use In Medicinementioning

confidence: 99%

“…In spite of encouraging results obtained with ozone therapy, its clinical use remains controversial due to the scarce knowledge of the mechanisms underlying its therapeutic action and the efficacy in heterogeneous diseases (Ajamieh et al, 2002;Gornicki & Gutsze, 2000). Currently, with reappraisal of ozone therapy, ozone has been utilized worldwide in research and clinical field.…”

Section: Ozone: Its General Effects and Use In Medicinementioning

confidence: 99%

“…It has recently been discovered that ozone is able to induce an adaptation to oxidative stress or promote an oxidative preconditioning through the increase and preservation of endogenous antioxidant systems (Ajamieh et al, 2002;. The adaptation develops after multiple ozone exposures (Ajamieh et al, 2002;Bocci et al, 2007).…”

Section: Hemorheological Effects Of Ozone Treatmentmentioning

confidence: 99%

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Similar protective effect of ischaemic and ozone oxidative preconditionings in liver ischaemia/reperfusion injury

Cited by 85 publications

References 32 publications

Effect of liver ischemic preconditioning in cirrhotic rats submitted to hepatic ischemia/reperfusion injury

Effect of liver ischemic preconditioning in cirrhotic rats submitted to hepatic ischemia/reperfusion injury

Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

How Ozone Treatment Affects Erythrocytes

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