Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and Their Exosomes for Damaged SVOG Ovarian Granulosa Cells

Abstract

Background. This study is aimed at investigating the repairing effect of mesenchymal stem cells and their exosomes from different sources on ovarian granulosa cells damaged by chemotherapy drugs—phosphoramide mustard (PM). Methods. In this study, we choose bone marrow mesenchymal stem cells (BMSCs) and human placental mesenchymal stem cells (HPMSCs) for research. Then, they were cocultured with human ovarian granulosa cells (SVOG) injured by phosphoramide mustard (PM), respectively. β-Galactosidase staining, flow cytometry, and Western blot were used to detect the changes in the senescence and apoptosis of SVOG cells before and after their coculture with the above two types of MSCs. Subsequently, exosomes from these two types of MSCs were extracted and added to the culture medium of SVOG cells after PM injury to test whether these two types of exosomes played a role similar to that of MSCs in repairing damaged SVOG cells. Results. PM treatment-induced apoptotic SVOG cells were significantly decreased after HPMSCs and BMSCs as compared with control group. After coculturing with these two types of MSCs, PM-treated SVOG cells showed significantly reduced senescence and apoptosis proportions as well as cleaved-Caspase 3 expression, and HPMSCs played a slightly stronger role than BMSCs in repairing SVOG cells in terms of the above three indicators. In addition, the ratios of senescent and apoptotic SVOG cells were also significantly reduced by the two types of exosomes, which played a role similar to that of MSCs in repairing cell damages. Conclusions. The results indicated that BMSCs, HPMSCs, and their exosomes all exerted a certain repair effect on SVOG cells damaged by PM, and consistent repair effect was observed between exosomes and MSCs. The repair effect of exosomes secreted from BMSCs and HPMSCs on the SVOG cells was studied for the first time, and the results fully demonstrated that exosomes are the key carriers for MSCs to play their role.

Expression of Long Noncoding RNAs in the Ovarian Granulosa Cells of Women with Diminished Ovarian Reserve Using High-Throughput Sequencing

“…Follicular reserve and ovarian function are measures of reproductive endocrine functionality and fertility potential (14). OGCs play important roles in the regulation of follicle activation, growth, development, and atresia (15). Recently, several studies targeting speci c genes and regulating various signaling pathways have shown that lncRNAs have vital roles in the formation and development of ovarian follicles (16-18).…”

Comparison between Intra-Articular Injection of Infrapatellar Fat Pad (IPFP) Cell Concentrates and IPFP-Mesenchymal Stem Cells (MSCs) for Cartilage Defect Repair of the Knee Joint in Rabbits

“…All data were expressed as the mean ± standard deviation (SD). Sample size was set based on the results of other previous studies [ 43 – 45 ]. Comparisons were performed with the Student t -test or one-way ANOVA for experiments with more than two subgroups.…”

“…All data were expressed as the mean ± standard deviation (SD). Sample size was set based on the results of other previous studies [43][44][45].…”

Potential of Mesenchymal Stem Cell-Derived Exosomes as a Novel Treatment for Female Infertility Caused by Bacterial Infections

“…Due to the unique life cycles of N. gonorrhoeae and C. trachomatis, the major PID caused by these infections are chronic, so antibiotic therapy is less effective, which often leads to persistence of the infection and reinfection (Chen et al, 2020). Various studies listed in Table 1 have reported the positive effects of MSC-Exos in the treatment of gynecological diseases (Sun et al, 2019;Zhang et al, 2019;Xin et al, 2020;Zhao et al, 2020;Lee et al, 2021;Liao et al, 2021).…”