Similarities and differences in supporting and chromaffin cells in the mammalian adrenal medullae: An immunohistochemical study

Suzuki, Takao; Kachi, Takashi

doi:10.1002/(sici)1097-0185(199603)244:3<358::aid-ar7>3.3.co;2-v

Cited by 13 publications

(22 citation statements)

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“…a), there are signals of comparable intensity in the cortex region and/or off tissue on the MALDI plate. These signals are likely due to chemical background or MALDI matrix interferences as these images are not consistent with predominant medulla localization obtained by other methods . We also investigated the more traditional method of post coating the tissues with CHCA matrix, as well as pre‐ and post coating using a different matrix ( trans ‐FA); however, these methods did not improve the images.…”

Section: Resultsmentioning

confidence: 95%

“…For preliminary tissue analysis, we focused on pig adrenal gland because the localizations of NTs/hormones, such as dopamine (DA), epinephrine (E) and norepinephrine (NE), have already been studied using immunohistochemical methods, radioisotopic techniques and DESI . Epinephrine (a.k.a.…”

Section: Resultsmentioning

confidence: 99%

“…adrenaline) and norepinephrine (a.k.a. noradrenaline) each localize to different chromaffin cell types, E cells and NE cells, respectively, which exhibit differential spatial localization within the central medulla region . Peripheral areas in the medulla of the pig adrenal gland are occupied with E cells and central regions with NE cells .…”

Section: Resultsmentioning

confidence: 99%

“…The derivatization protocol resulted in images where the analytes showed predominant signal localization to the medulla region, which is consistent with the biological function of each . In addition, norepinephrine and epinephrine localize to different regions in the medulla consistent with previously published data …”

Section: Resultsmentioning

confidence: 99%

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A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

et al. 2014

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Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity. Here we present approaches for the improvement in detection of endogenous amine metabolites such as amino acids and neurotransmitters in tissues through chemical derivatization and matrix-assisted laser desorption/ionization (MALDI) IMS. Chemical derivatization with 4-hydroxy-3-methoxycinnamaldehyde (CA) was used to improve sensitivity and specificity. CA was applied to the tissue via MALDI sample targets precoated with a mixture of derivatization reagent and ferulic acid (FA) as a MALDI matrix. Spatial distributions of chemically derivatized endogenous metabolites in tissue were determined by high-mass resolution and MSn imaging mass spectrometry. We highlight an analytical strategy for metabolite validation whereby tissue extracts are analyzed by high-performance liquid chromatography (HPLC)-MS/MS to unambiguously identify metabolites and distinguish them from isobaric compounds.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

et al. 2014

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“…Examples for normal chromaffin cells include varied cell populations and tissue organization in vivo , varied responsiveness to neurotrophic or mitogenic factors in vitro , and utilization of varied signal transducers in physiological processes. Normal rodent and bovine adrenals are characterized by separate E‐ and NE‐cells,7 while human adrenal medulla does not show such separation 8. Adult rat chromaffin cells show a predominantly proliferative response to NGF in cell culture,9 while their counterparts from adult humans undergo neuronal differentiation without proliferation,10 and those from adult mice show essentially no response 11.…”

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confidence: 99%

Chromaffin Cells as Models of Endocrine Cells and Neurons

Tischler

2002

Annals of the New York Academy of Sciences

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Chromaffin cells have many functional similarities to amine- and peptide-producing endocrine cells throughout the body and to both peripheral and central neurons. The hypothesis of a shared, neural origin for chromaffin cells and most other endocrine cells is not tenable. However, chromaffin cells and their neoplastic counterparts, known as pheochromocytomas, are valuable models for studies of endocrine and neural properties. In this session, PC12 rat pheochromocytoma cells are used in two novel applications: to identify profiles of gene expression that may mediate cell death in neurodegenerative disorders and to study mechanisms for transduction of hypoxic signals. Recently described pheochromocytoma cell lines from neurofibromatosis knockout mice are shown to be novel models for signaling by the receptor tyrosine kinase ret, and purified enterochromaffin-like (ECL) cells are shown to offer new opportunities to study the shared and distinctive aspects of neuroendocrine function using a normal cell type.

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Metastasis‐associated S100A4 (Mts1) protein is expressed in subpopulations of sensory and autonomic neurons and in Schwann cells of the adult rat

Sandelin

Zabihi

Liu

et al. 2004

J of Comparative Neurology

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S100A4 (Mts1) is a member of a family of calcium-binding proteins of the EF-hand type, which are widely expressed in the nervous system, where they appear to be involved in the regulation of neuron survival, plasticity, and response to injury or disease. S100A4 has previously been demonstrated in astrocytes of the white matter and rostral migratory stream of the adult rat. After injury, S100A4 is markedly up-regulated in affected central nervous white matter areas as well as in the periventricular area and rostral migratory stream. Here, we show that S100A4 is expressed in a subpopulation of dorsal root, trigeminal, geniculate, and nodose ganglion cells; in a subpopulation of postganglionic sympathetic and parasympathetic neurons; in chromaffin cells of the adrenal medulla; and in satellite and Schwann cells. In dorsal root ganglia, S100A4-positive cells appear to constitute a subpopulation of small ganglion neurons, a few of which coexpressed calcitonin gene-related peptide (CGRP) and Griffonia simplicifolia agglutinin (GSA) isolectin B4 (B4). S100A4 protein appears to be transported from dorsal root ganglia to the spinal cord, where it is deposited in the tract of Lissauer. After peripheral nerve or dorsal root injury, a few S100A4-positive cells coexpress CGRP, GSA, or galanin. Peripheral nerve or dorsal root injury induces a marked up-regulation of S100A4 expression in satellite cells in the ganglion and in Schwann cells at the injury site and in the distal stump. This pattern of distribution partially overlaps that of the previously studied S100B and S100A6 proteins, indicating a possible functional cooperation between these proteins. The presence of S100A4 in sensory neurons, including their processes in the central nervous system, suggests that S100A4 is involved in propagation of sensory impulses in specific fiber types.

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Similarities and differences in supporting and chromaffin cells in the mammalian adrenal medullae: An immunohistochemical study

Cited by 13 publications

References 0 publications

A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

Chromaffin Cells as Models of Endocrine Cells and Neurons

Metastasis‐associated S100A4 (Mts1) protein is expressed in subpopulations of sensory and autonomic neurons and in Schwann cells of the adult rat

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