Similarities of biophysical properties of several human enteroviruses as shown by density gradient ultra centrifugation of mixtures of the viruses

Schaffer, Frederick L.; Frommhagen, Laurence H.

doi:10.1016/0042-6822(65)90095-4

Cited by 31 publications

(8 citation statements)

References 9 publications

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“…Centrifugation of the picornaviruses for 270 min at 39,000 rev/min in the ultracentrifuge resulted in the sedimentation of these viruses to an equilibrium position. The buorant density determined for poliovirus 1 by the methods used in this study is in agreement with the values determined for the picornaviruses by Schaffer and Frommhagan (17). These workers mixed their virus suspensions with CsCl solutions to achieve a final density of 1.34 g/cc, and centrifuged this mixture for 18 to 24 hr at 30,000 rev/min.…”

Section: Discussionsupporting

confidence: 88%

Comparative Study of Seven Picornaviruses of Man

Jamison

Mayor

1966

J Bacteriol

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The buoyant densities in CsCl and potassium tartrate of seven acid-resistant picornaviruses of man were determined. The viruses studied were found to be a relatively homogeneous group. Buoyant densities ranged from 1.32 to 1.35 g/cc in CsCl and 1.21 to 1.23 g/cc in potassium tartrate. The buoyant density of each virus did not vary by more than 0.01 g/cc. The host cell of origin and passage level did not influence the buoyant density of the virions. However, measurements of the diameters of purified virions by a standard method have shown that there are considerable size variations among these picornaviruses. The diameters ranged from 18 to 25 m,u. Echovirus 19 and poliovirus 1 were significantly larger (mean diameter, 23.5 m,u) than echoviruses 4, 6, 16, and 23, and coxsackievirus B2 (mean diameter, 21.0 m,u).

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Section: Discussionsupporting

confidence: 88%

Comparative Study of Seven Picornaviruses of Man

Jamison

Mayor

1966

J Bacteriol

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“…Our findings of substantial average proportions of HAV banding at buoyant densities of 1.36-1.37, 1.40-1.42, and 1.45-1.48 g/cm3 in CsCl are not consistent with the notion that HAV is an enterovirus. Whereas enteroviruses characteristically band in CsCl at a buoyant density of 1.34 g/cm3, parvoviruses appear to possess multiple buoyant densities, normally banding in CsCl gradients at higher densities, including 1.35-1.37, 1.40-1.42, and 1.45-1.47 g/cm3 (14,15,26,(28)(29)(30). In this respect, the banding characteristics of HAV described here are more like those of parvoviruses.…”

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confidence: 63%

Multiple buoyant densities of hepatitis A virus in cesium chloride gradients

Bradley

McCaustland

Schreeder

et al. 1977

Journal of Medical Virology

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Hepatitis A virus (HAV) recovered from stools of human cases of hepatitis A and from stools of chimpanzees experimentally infected with HAV was shown to possess multiple buoyant densities in CsCl gradients. The greatest proportion of HAV was most frequently found at a buoyant density of 1.32-1.34 g/cm3, however, large proportions of HAV were also frequently found at higher densities, including 1.36-1.37, 1.40-1.42, and 1.45-1.48 g/cm3. These findings are consistent with the notion that HAV may be a parvovirus.

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“…The binding of water and cesium ions by vimses is known to alter their densities (31). In this regard, it is of interest that the density of poliovirus in cesium chloride (1.34) is very close to its calculated hydrated density (1.32), indicating that there is no appreciable binding of cesium ions (23). Experiments involving isopycnic banding in other density gradient materials and direct chemical analysis of purified rhiiioviruses are required before the basis for the high density of these viruses can be understood.…”

mentioning

confidence: 99%