Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid

Meyers, Jade; Sanchez, Daniel R.; Elwell, Lynn P.; Falkow, Stanley

doi:10.1128/jb.127.3.1529-1537.1976

Cited by 1,063 publications

(322 citation statements)

References 14 publications

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“…Plasmid DNA samples were electrophoresed through 0·7% agarose in TBE buffer (Meyers et al 1976). The gels were stained with ethidium bromide solution, discoloured in water, and photographed.…”

Section: Plasmid Analysismentioning

confidence: 99%

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Martins

Bauab

Leite

et al. 2007

Mem. Inst. Oswaldo Cruz

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Section: Plasmid Analysismentioning

confidence: 99%

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Martins

Bauab

Leite

et al. 2007

Mem. Inst. Oswaldo Cruz

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“…Arguably the best understood mobile genetic elements are plasmids, which have been widely studied in S. aureus and MRSA since the 1970s [7][8][9][10]. Currently, it is suggested that more than 90% of MRSA strains carry plasmids while numerous studies have supported the important role plasmids play in staphylococcal multidrug-resistance [7,[11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of methicillin-resistantStaphylococcus aureusreveals an absence of plasmid DNA in multidrug-resistant isolates

Caddick

Hilton

Rollason

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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The number, diversity and restriction enzyme fragmentation patterns of plasmids harboured by 44 multidrug-resistant hospital-acquired methicillin-resistant Staphylococcus aureus (MR-HA-MRSA) isolates, two multidrug-resistant community-acquired MRSA (MR-CA-MRSA), 50 hospital-acquired MRSA (HA-MRSA) isolates (from the University Hospital Birmingham, NHS Trust, UK) and 34 community-acquired MRSA (CA-MRSA) isolates (from general practitioners in Birmingham, UK) were compared. In addition, pulsed-field gel electrophoresis (PFGE) type following SmaI chromosomal digest and SCCmec element type assignment were ascertained for each isolate. All MR-HA-MRSA and MR-CA-MRSA isolates possessed the type II SCCmec, harboured no plasmid DNA and belonged to one of five PFGE types. Forty-three out of 50 HA-MRSA isolates and all 34 CA-MRSA isolates possessed the type IV SCCmec and all but 10 of the type IV HA-MRSA isolates and nine CA-MRSA isolates carried one or two plasmids. The 19 non-multidrug-resistant isolates (NMR) that did not harbour plasmids were only resistant to methicillin whereas all the NMR isolates harbouring at least one plasmid were resistant to at least one additional antibiotic. We conclude that although plasmid carriage plays an important role in antibiotic resistance, especially in NMR-HA-MRSA and CA-MRSA, the multidrug resistance phenotype from HA-MRSA is not associated with increased plasmid carriage and indeed is characterised by an absence of plasmid DNA.

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“…1). In contrast to the results of previous were identified by their sub-division into two workers (Meyers et al 1976 Molecular weights were calculated using the plasmids RIGQ-I, RPI, R388, pSC134, pHV14 and pBR322 as molecular weight standards. oc DNA bands were identified by a modification of the method of Hintermann et al (1981).…”

Section: Form (Ccc) and Open Circular (Oc) Form Thusmentioning

confidence: 99%

The R‐factors of multiple antibiotic resistant faecal coliforms isolated from a domestic dog

Minton¹,

Marsh²,

Atkinson³

1983

Journal of Applied Bacteriology

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The antibiotic resistant faecal flora of a domestic dog suffering from an acute enteric infection was examined. The flora exhibited overall resistance to a wide variety of antibiotics. However, following restoration of the animal to normal health, overall resistance to ampicillin (Ap), tetracycline (Tc), chloramphenicol (Cm) and streptomycin Sm was lost, although low numbers of bacteria resistant to these four antimicrobial agents could still be isolated up to one year later. A total of 11 strains were purified for further study. All 11 were positively identified as Escherichia coli and shown to be resistant to various combinations of the above antibiotics, and additionally to kanamycin (Km). Each strain harboured from one to five plasmids, although only four proved capable of transferring antibiotic resistance to Escherichia coli K-12. One of the strains was found to harbour two conjugal plasmids pNJ101 (60 Md) and pNJ102 (133 Md) which coded for resistance to Cm, Tc, Ap and Cm, Tc, Km respectively. A third plasmid pNJ103 (29 Md) remains cryptic. The possession of the two plasmids pNJ101 and pNJ102 appears to be an unstable situation as variants arose harbouring one or other of the plasmids.

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Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid

Cited by 1,063 publications

References 14 publications

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Molecular analysis of methicillin-resistantStaphylococcus aureusreveals an absence of plasmid DNA in multidrug-resistant isolates

The R‐factors of multiple antibiotic resistant faecal coliforms isolated from a domestic dog

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