Simple aminophenol-based electrochemical probes for non-enzymatic, dual amperometric detection of NADH and hydrogen peroxide

Thiruppathi, Murugan; Lin, Pei‐Ying; Chou, Yi-Te; Ho, Hsin‐Yu; Wu, Li-Chen; Ho, Ja‐an Annie

doi:10.1016/j.talanta.2019.03.083

Cited by 52 publications

(26 citation statements)

References 43 publications

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“…CV measurements were done in 0.1 M, pH 7.4 PB solution under the potential sweeping from 0 to −1.2 V at a scan rate of 100 mV s −1 . During the cathodic segment, H2O2 reduction peak [48] was noticed at ~−0.7 V for SPCE and for SPCE/NPCT the same reduction peak was noticed with lower over potential (~−0.4 V). Among the various electrodes, detection response are clearer and more explicit at SPCE/NPC600.…”

Section: Detection Of H2o2 At Spce/npctmentioning

confidence: 54%

Carbonization and Preparation of Nitrogen-Doped Porous Carbon Materials from Zn-MOF and Its Applications

et al. 2020

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Nitrogen-doped porous carbon (NPC) materials were successfully synthesized via a Zn-containing metal-organic framework (Zn-MOF). The resulting NPC materials are characterized using various physicochemical techniques which indicated that the NPC materials obtained at different carbonization temperatures exhibited different properties. Pristine MOF morphology and pore size are retained after carbonization at particular temperatures (600 °C-NPC600 and 800 °C-NPC800). NPC800 material shows an excellent surface area 1192 m2/g, total pore volume 0.92 cm3/g and displays a higher CO2 uptake 4.71 mmol/g at 273 k and 1 bar. Furthermore, NPC600 material displays good electrochemical sensing towards H2O2. Under optimized conditions, our sensor exhibited a wide linearity range between 100 µM and 10 mM with a detection limit of 27.5 µM.

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Section: Detection Of H2o2 At Spce/npctmentioning

confidence: 54%

Carbonization and Preparation of Nitrogen-Doped Porous Carbon Materials from Zn-MOF and Its Applications

et al. 2020

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“…Nevertheless, hydrogen peroxide is known for its strong inhibition effects. [6] Therefore, we decided to check its influence on enzyme activity. As the reaction mediated by Novozym 435 proceeded with the lowest speed, we conducted a series of experiments in time, for Novozym 435 and increased the amount of hydrogen peroxide.…”

Section: Enzymementioning

confidence: 99%

“…[5] However, the presence of hydrogen peroxide influences the activity of certain enzymes. [6] Therefore, despite its essential role in controlling various processes, the unregulated production or mismanagement of these oxygen-containing molecules [2] leads to the destruction of tissue and organs. [7] The excess of hydrogen peroxide can damage nucleic acids or proteins.…”

Section: Introductionmentioning

confidence: 99%

Efficient Assay for the Detection of Hydrogen Peroxide by Estimating Enzyme Promiscuous Activity in the Perhydrolysis Reaction

Wilk

Ostaszewski

2021

ChemBioChem

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Hydrogen peroxide is an ideal oxidant in view of its availability, atom economy, or green aspects. Furthermore, it is produced by the cell mitochondria and plays a meaningful role in controlling physiological processes, but its unregulated production leads to the destruction of organs. Due to its diverse roles, a fast and selective method for hydrogen peroxide detection is the major limitation to preventing the negative effects caused by its excess. Therefore, we aimed to develop an efficient assay for the detection of H 2 O 2 . For this purpose, we combined the enzymatic method for the detection of hydrogen peroxide with the estimation of the promiscuity of various enzymes. We estimated the activity of an enzyme in the reaction of pnitrophenyl esters with hydrogen peroxide resulting in the formation of peracid. To our knowledge, there is no example of a simple, multi-sensor demonstrating the promiscuous activity of an enzyme and detecting hydrogen peroxide in aqueous media.

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“…Nicotine amide adenine dinucleotide (NADH) is an important co-enzyme in living cells which has essential roles in biological systems [1][2][3]. As determination of NADH is helpful in diagnostic of many sicknesses such as cancer and diabetes, therefore a lot of scientists in all around the word are trying to develop accurate, precise and cost-effective methods for NADH monitoring [4].…”

Section: Introductionmentioning

confidence: 99%

Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples

Mohammad‐Rezaei

Golmohammadpour

2019

Electroanalysis

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In this study, a sensitive nicotinamide adenine dinucleotide (NADH) biosensor based on Au‐Copper oxide nanocomposite modified carbon ceramic electrode (Au−CuO/CCE) was introduced. The developed NADH biosensor was prepared by controlled electrodeposition of copper and Au nanoparticles on the surface of a renewable CCE and was turned to Au−CuO/CCE by cycling the potential in alkaline media. The prepared electrode was carefully characterized with scanning electron microscopy, X‐ray diffraction, atomic force microscopy and cyclic voltammetry techniques. According to scan rate study, surface coverage (Γ) of the fabricated Au−CuO/CCE was calculated to be 1.54×10−8 mol cm−2 which was 3 time more than CuO/CCE. The fabricated electrode is well stable which could be reliably utilized for the determination of NADH with amperometry technique over the concentration range of 1–29 μM with sensitivity and detection limit (S/N=3) of 0.1025 μA μM−1 and 0.09 μM respectively. The prepared biosensor was used for NADH determination in serum samples with fast response time and satisfactory analytical results.

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Simple aminophenol-based electrochemical probes for non-enzymatic, dual amperometric detection of NADH and hydrogen peroxide

Cited by 52 publications

References 43 publications

Carbonization and Preparation of Nitrogen-Doped Porous Carbon Materials from Zn-MOF and Its Applications

Carbonization and Preparation of Nitrogen-Doped Porous Carbon Materials from Zn-MOF and Its Applications

Efficient Assay for the Detection of Hydrogen Peroxide by Estimating Enzyme Promiscuous Activity in the Perhydrolysis Reaction

Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples

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