Simple and direct detection of Staphylococcus aureus in milk by a tube coagulase test

Yazdankhah, Siamak Pour; Olsen, Egil

doi:10.1046/j.1472-765x.1998.00395.x

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Hence, a total of 32 bacterial isolates were obtained on pure culture. The higher proportion of culture negative samples might be due to the inhibitory action of the endogenous antibacterial substances present in milk (Yazdankhah and Olsen, 1998). It could also be attributed to the non-bacterial etiology that could not be isolated by streaking on to a bacteriological medium (Wellenberg et al, 2002).…”

Section: Isolation and Identification Of Bacteria From Subclinical Ma...mentioning

confidence: 99%

Identification of the etiology of subclinical mastitis in an organised dairy herd

Jose¹,

Vijayakumar²,

K.³

et al. 2022

JIVA

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The study describes the identification of the etiological agents of subclinical mastitis (SCM) among lactating dairy cows under different phases of lactation in an organised dairy farm of Thrissur district, Kerala. The preliminary diagnosis of subclinical mastitis (SCM) was made using California Mastitis test (CMT), a cow side field test. The somatic cell count (SCC) was estimated and the milk samples of affected quarters were subjected to bacterial isolation and identification. The predominant causative agents isolated from milk of cows with SCM were coagulase negative staphylococci (56.25 per cent) followed by S. aureus (25 per cent) and Micrococci spp. (18.75 per cent). Further species identification of the coagulase negative staphylococci revealed that the majority were S. xylosus (28.23 per cent) followed by S. scuiri (15.63 per cent), S. hominis (9.38 per cent) and S. schleiferi (3.13 per cent).

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Section: Isolation and Identification Of Bacteria From Subclinical Ma...mentioning

confidence: 99%

Identification of the etiology of subclinical mastitis in an organised dairy herd

Jose¹,

Vijayakumar²,

K.³

et al. 2022

JIVA

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“…Although detection methodology continues to improve, microbiological culture is still the recommended method (2) for determining the bacterial content of a milk sample, despite this procedure being both time-consuming and expensive. Consequently, more rapid methods of identification and/or enumeration of bacteria in milk are being developed (3,5). Many of these techniques, e.g., PCR-based methods, can be completed in a matter of hours, but they lack the specificity to be able to determine the bacterial species present or are unable to enumerate the bacteria present.…”

Section: Letter To the Editor Savinase Is A Bactericidal Enzymementioning

confidence: 99%

Savinase Is a Bactericidal Enzyme

Smith

Green

Mason

2003

Appl Environ Microbiol

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Frequência de Staphylococcus aureus em casos de mastite bovina subclínica, na região Sul do Rio Grande do Sul

et al. 2013

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RESUMOA mastite bovina é uma doença importante na exploração leiteira, não apenas pelas perdas econômicas diretas que promove, mas também pelas perdas indiretas e o potencial risco à saúde pública. Dentre as principais causas de infecções intramamárias, destacam-se as bactérias do gênero Staphylococcus spp., sendo que Staphylococcus aureus é o agente etiológico predominante em mastite subclínica. O objetivo desse trabalho foi verificar a frequência de mastite subclínica em oito rebanhos localizados na região Sul do Rio Grande do Sul (Brasil) e a relação da enfermidade com a presença de S. aureus. Adicionalmente, pesquisou-se a presença de S. intermedius e S. hyicus nas amostras de leite obtidas. Para identificação da doença, utilizou-se o California Mastitis Test (CMT). A identificação da espécie de Staphylococcus spp. foi feita em meio de cultura ágar BairdParker, com posterior confirmação das colônias suspeitas em coloração de gram, prova de catalase, pesquisa de coagulase livre e pesquisa de termonuclease. A mastite subclínica foi constatada em 53,6% dos animais testados. A presença de Staphylococcus coagulase positiva foi identificada em 12,6% dos animais com mastite subclínica. Nesses mesmos animais, a bactéria identificada como S. aureus foi o agente etiológico presente em 17,6% dos casos. Adicionalmente, pode-se perceber que, dentre o grupo identificado como coagulase positiva, 85,7% corresponderam a S. aureus, enquanto 8,5% mostraram características bioquímicas compatíveis com S. intermedius e 5,8% foram consideradas S. hyicus. PALAVRAS-CHAVE:Coagulase, leite, intermedius, hyicus. ABSTRACT FREQUENCY OF STAPHYLOCOCCUS AUREUS FROM BOVINE SUBCLINICAL MASTITIS CASES, IN SOUTHERN RIO GRANDE DO SUL, BRAZIL.Bovine mastitis is an important disease in dairy farming, not only by promoting direct economic losses, but also for indirect losses and the potential risk to public health. The main causes of intramammary infections include the bacteria of the genus Staphylococcus spp., Staphylococcus aureus being the predominant etiologic agent in subclinical mastitis. The aim of this study was to determine the frequency of subclinical mastitis in eight herds from southern Rio Grande do Sul (Brazil) and the relationship of the disease with the presence of S. aureus. In addition, we checked for the presence of S. intermedius and S. hyicus in the milk samples obtained. For identification of the disease, we used the California Mastitis Test (CMT). Identification of Staphylococcus spp. species was made in BaridParker agar culture medium, with subsequent confirmation of suspected colonies by way of Gram stain and catalase test along with free-coagulase and thermonuclease research. Subclinical mastitis was identified in 53.6% of animals tested. The presence of coagulase-positive Staphylococcus was identified in 12.6% of animals with subclinical mastitis. In these same animals, bacteria identified as S. aureus were the etiologic agent present in 17.6% of cases. Additionally, it was revealed that among the group identified as coag...

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Simple and direct detection of Staphylococcus aureus in milk by a tube coagulase test

Cited by 3 publications

References 12 publications

Identification of the etiology of subclinical mastitis in an organised dairy herd

Identification of the etiology of subclinical mastitis in an organised dairy herd

Savinase Is a Bactericidal Enzyme

Frequência de Staphylococcus aureus em casos de mastite bovina subclínica, na região Sul do Rio Grande do Sul

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