Simple and efficient approach for improved cytocompatibility and faster degradation of electrospun polycaprolactone fibers

Bhaskaran, Aswathy; Prasad, Tilak; Kumary, T. V.; Kumar, P.R. Anil

doi:10.1007/s00289-018-2442-7

Cited by 13 publications

(6 citation statements)

References 24 publications

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“…These results are in line with what is seen in the literature from PCL 33 and PDLLA 34 -based scaffolds. Acrylated materials are commonly used in biomedical applications with good cytocompatibility, 35 and similar tests have been conducted on other AUP polymers with similar results 20,36 so there is a precedent for the use of such materials in a biomedical application.…”

Section: Cell Viability Studysupporting

confidence: 93%

Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

Darroch

Asaro

Gréant

et al. 2023

J Biomedical Materials Res

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The use of polymeric biomaterials to create tissue scaffolds using additive manufacturing techniques is a well‐established practice, owing to the incredible rapidity and complexity in design that modern 3D printing methods can provide. One frontier approach is melt electrowriting (MEW), a technique that takes advantage of electrohydrodynamic phenomena to produce fibers on the scale of 10's of microns with designs capable of high resolution and accuracy. Poly(ε‐caprolactone) (PCL) is a material that is commonly used in MEW due to its favorable thermal properties, high stability, and biocompatibility. However, one of the drawbacks of this material is that it lacks the necessary chemical groups which allow covalent crosslinking of additional elements onto its structure. Attempts to functionalise PCL structures therefore often rely on the functional units to be applied externally via coatings or integrally mixed elements. Both can be extremely useful depending on their applications, but can add extra difficulties into the use of the resulting structures. Coatings require careful design and application to prevent rapid degradation, while elements mixed into the polymer melt must deal with the possibilities of phase separation and changes to MEW properties of the unadulterated polymer. With this in mind, this study sought to imbibe functionality to MEW‐printed scaffolds using the approach of adding functional units directly, via covalent bonding of functional groups to the polymer itself. To this end, this study employs a recently developed class of polymers called acrylate‐endcapped urethane‐based polymers (AUPs). The polymer backbone of the specific AUP used consists of a poly(D,L‐lactic acid) (PDLLA)/PCL copolymer chain, which is functionalized with 6 acrylate groups, 3 at either end. Through blending of the AUP with PCL, various concentrations of this mixture were used with MEW to produce scaffolds that possessed acrylate groups on their surface. Using UV crosslinking, these groups were tagged with Fluorescein‐o‐Acrylate to verify that PDLLA/PCL AUP/PCL blends facilitate the direct covalent bonding of external agents directly onto the MEW material. Blending of the AUP with PCL increases the scaffold's stiffness and ultimate strength. Finally, blends were proven to be highly biocompatible, with cells attaching and proliferating readily at day 3 and 7 post seeding. Through this work, PDLLA/PCL AUP/PCL blends clearly demonstrated as a biocompatible material that can be processed using MEW to create functionalised tissue scaffolds.

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Section: Cell Viability Studysupporting

confidence: 93%

Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

Darroch

Asaro

Gréant

et al. 2023

J Biomedical Materials Res

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“…PCL is a hydrophobic semi-crystalline polymer with a slow degradation rate (2–4 years) and thus provides good stability. , A recent hydrolytic degradation study confirmed there was no significant effect on the morphology of PCL electrospun fibers after 90 days of degradation . However, studies have shown PCL fibers degrade faster in enzymatic media and in vivo conditions as well as blending with other biomaterials such as PLA, PLLA, chitosan, gelatin, and cellulose, and faster degradation can also be achieved by surface modification. − Our results show that fiber topography was maintained after a 14 day incubation in culture media with slight reductions in weight, mechanical stiffness, and UTS. This demonstrates the stability of our system over short-term culture periods in hydrolytic conditions.…”

Section: Discussionmentioning

confidence: 53%

A Unification of Nanotopography and Extracellular Matrix in Electrospun Scaffolds for Bioengineered Hepatic Models

Gao

Bate

Callanan

2023

ACS Appl. Bio Mater.

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Donor liver shortage is a crucial global public health problem as whole-organ transplantation is the only definitive cure for liver disease. Liver tissue engineering aims to reproduce or restore function through in vitro tissue constructs, which may lead to alternative treatments for active and chronic liver disease. The formulation of a multifunctional scaffold that has the potential to mimic the complex extracellular matrix (ECM) and their influence on cellular behavior, are essential for culturing cells on a construct. The separate employment of topographic or biological cues on a scaffold has both shown influences on hepatocyte survival and growth. In this study, we investigate both of these synergistic effects and developed a new procedure to directly blend whole-organ vascular perfusion-decellularized rat liver ECM (dECM) into electrospun fibers with tailored surface nanotopography. Water contact angle, tensile test, and degradation studies were conducted to analyze scaffold hydrophilicity, mechanical properties, and stability. The results show that our novel hybrid scaffolds have enhanced hydrophilicity, and the nanotopography retained its original form after hydrolytic degradation for 14 days. Human hepatocytes (HepG2) were seeded to analyze the scaffold biocompatibility. Cell viability and DNA quantification imply steady cell proliferation over the culture period, with the highest albumin secretion observed on the hybrid scaffold. Scanning electron microscopy shows that cell morphology was distinctly different on hybrid scaffolds compared to control groups, where HepG2 began to form a monolayer toward the end of the culture period; meanwhile, typical hepatic markers and ECM genes were also influenced, such as an increasing trend of albumin appearing on the hybrid scaffolds. Taken together, our findings provide a reproducible approach and utilization of animal tissue-derived ECM and emphasize the synergism of topographical stimuli and biochemical cues on electrospun scaffolds in liver tissue engineering.

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“…Many previous studies have proven that NaOH corrosion is an effective strategy because it can improve the PCL surface roughness and cytocompatibility [ 38 , 39 ]. In a comparative study, Zamani et al compared the effect of NaOH to RGD-induced modifications.…”

Section: Resultsmentioning

confidence: 99%

3D-printed PCL scaffolds with anatomy-inspired bionic stratified structures for the treatment of growth plate injuries

Wang,

Li,

Liu

et al. 2023

Materials Today Bio

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Simple and efficient approach for improved cytocompatibility and faster degradation of electrospun polycaprolactone fibers

Cited by 13 publications

References 24 publications

Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

A Unification of Nanotopography and Extracellular Matrix in Electrospun Scaffolds for Bioengineered Hepatic Models

3D-printed PCL scaffolds with anatomy-inspired bionic stratified structures for the treatment of growth plate injuries

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