Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays

Lee, Dohwan; Shin, Yong Moon; Chung, Seok Won; Hwang, Kyo Seon; Yoon, Dae Sung

doi:10.1021/acs.analchem.6b03460

Cited by 78 publications

(80 citation statements)

References 36 publications

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“…The LAMP assay can be performed using either a one-step or two-step protocol directly from sample matrices, including serum, semen, urine, saliva, and mosquitoes homogenate. The use of Bst 2.0 polymerase, Bst 3.0 polymerase or OmniAmp polymerase enables the test to be performed in a one-step protocol [38,[40][41][42]53,65] (Figure 2). These enzymes have both reverse transcriptase (RT) and DNA polymerase activities at a fixed temperature (50-72 °C), and so can be run isothermally for Abbreviations: aM, attomolar; PFU, plaque-forming unit; FFU, focus forming units; TCID 50 , 50% tissue culture infective dose.…”

Section: Step 1: Template Preparationmentioning

confidence: 99%

“…These enzymes have both reverse transcriptase (RT) and DNA polymerase activities at a fixed temperature (50-72 • C), and so can be run isothermally for the direct detection of RNA or DNA targets. It is the robust nature of these enzymes, which are capable of maintaining their activities even in the presence of reaction inhibitors, that enables the assay to be performed in the field without the need to extract the genetic material from the samples [38,40,65]. Based on this advantage, several one-step LAMP assays have been developed for the detection of ZIKV [41,42,53].…”

Section: Step 1: Template Preparationmentioning

confidence: 99%

“…Similar to the Bst DNA polymerase 2.0, Bst DNA polymerase 3.0 is an in vitro derivative of Bacillus stearothermophilus Bst DNA polymerase and features further improved amplification reaction properties compared to Bst 2.0. The enzyme has been successfully used for rapid ZIKV detection in both human and mosquito samples [40,53]. Bst DNA polymerase 3.0 exhibits DNA polymerase activity 5 -3 with either DNA or RNA targets, strong strand displacement activity, improved RT activity compared to Bst DNA polymerase 2.0 and lacks both 5 -3 and 3 -5 exonuclease activity.…”

Section: Step 1: Template Preparationmentioning

confidence: 99%

“…Bst DNA polymerase 3.0 exhibits DNA polymerase activity 5 -3 with either DNA or RNA targets, strong strand displacement activity, improved RT activity compared to Bst DNA polymerase 2.0 and lacks both 5 -3 and 3 -5 exonuclease activity. Importantly, Bst DNA polymerase 3.0 displays robust performance even in high concentrations of reaction inhibitors, which has enabled LAMP applications for the ZIKV even without sample pre-treatment or RNA extraction [40,53]. These advantages make Bst DNA polymerase 3.0 an excellent candidate for POC platforms and the rapid detection of ZIKV and other pathogens.…”

Section: Step 1: Template Preparationmentioning

confidence: 99%

“…Importantly, the simple, single-temperature incubation allows LAMP reactions to be performed without expensive equipment, directly in the field [36]. Since the 2015 emergence of the ZIKV in Brazil, many LAMP assays have been developed for diagnosis by research groups across the world [27,[37][38][39][40][41][42][43][44][45]. These diagnostic platforms based on LAMP have proven to be specific, sensitive and inexpensive POC tools that can be applied even in resource-limited regions of the world.…”

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Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Section: Step 1: Template Preparationmentioning

confidence: 99%

Section: Step 1: Template Preparationmentioning

confidence: 99%

Section: Step 1: Template Preparationmentioning

confidence: 99%

Section: Step 1: Template Preparationmentioning

confidence: 99%

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confidence: 99%

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Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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Recent progresses and remaining challenges for the detection of Zika virus

Zhang

Chen

et al. 2021

Medicinal Research Reviews

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Zika virus (ZIKV) has emerged as a particularly notorious mosquito‐borne flavivirus, which can lead to a devastating congenital syndrome in the fetuses of pregnant mothers (e.g., microcephaly, spasticity, craniofacial disproportion, miscarriage, and ocular abnormalities) and cause the autoimmune disorder Guillain‐Barre' syndrome of adults. Due to its severity and rapid dispersal over several continents, ZIKV has been acknowledged to be a global health concern by the World Health Organization. Unfortunately, the ZIKV has recently resurged in India with the potential for devastating effects. Researchers from all around the world have worked tirelessly to develop effective detection strategies and vaccines for the prevention and control of ZIKV infection. In this review, we comprehensively summarize the most recent research into ZIKV, including the structural biology and evolution, historical overview, pathogenesis, symptoms, and transmission. We then focus on the detection strategies for ZIKV, including viral isolation, serological assays, molecular assays, sensing methods, reverse transcription loop mediated isothermal amplification, transcription‐mediated amplification technology, reverse transcription strand invasion based amplification, bioplasmonic paper‐based device, and reverse transcription isothermal recombinase polymerase amplification. To conclude, we examine the limitations of currently available strategies for the detection of ZIKV, and outline future opportunities and research challenges.

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Paper-Based Biosensors with Lateral/Vertical Flow Assay

Lee

2020

Bioanalysis

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Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays

Cited by 78 publications

References 36 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Recent progresses and remaining challenges for the detection of Zika virus

Paper-Based Biosensors with Lateral/Vertical Flow Assay

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