Simple and inexpensive long-term preservation methods for Phytophthora infestans

Cui, Haichen; Ren, Xingbo; Yun, Lisha; Hou, Qian; Feng, Fan; Liu, Huirong

doi:10.1016/j.mimet.2018.07.020

Cited by 8 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. infestans is soilborne and seedborne and infects all parts of the plant, including stems, leaves, and tubers, resulting in significant economic losses in the field and storage estimated as over 6 billion USD per year worldwide. 3 The infection of potato tubers in the field occurs by the spread of the pathogen's spores through underground stems, stolons, or roots during the growing season. Indirect infection also may come during or after harvest via handling of tubers in storage or contact between infected tubers and healthy tubers.…”

Section: ■ Introductionmentioning

confidence: 99%

Trichoderma Volatile Organic Compounds as a Biofumigation Tool against Late Blight Pathogen Phytophthora infestans in Postharvest Potato Tubers

Elsherbiny

Amin

Aleem

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

We tested the ability of 14 strains of Trichoderma to emit volatile compounds that decreased or stopped the growth of Phytophthora infestans. Volatile organic compounds (VOCs) emitted from Trichoderma strains designated T41 and T45 inhibited the mycelial growth of P. infestans grown on a laboratory medium by 80 and 81.4%, respectively, and on potato tubers by 93.1 and 94.1%, respectively. Using the DNA sequence analysis of the translation elongation factor region, both Trichoderma strains were identified as Trichoderma atroviride. VOCs emitted by the strains were analyzed, and 39 compounds were identified. The most abundant compounds were 3-methyl-1-butanol, 6-pentyl-2-pyrone, 2-methyl-1-propanol, and acetoin. Electron microscopy of the hyphae treated with T. atroviride VOCs revealed serious morphological and ultrastructural damages, including cell deformation, collapse, and degradation of cytoplasmic organelles. To our knowledge, this is the first report describing the ability of Trichoderma VOCs to suppress the growth of the late blight potato pathogen.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Trichoderma Volatile Organic Compounds as a Biofumigation Tool against Late Blight Pathogen Phytophthora infestans in Postharvest Potato Tubers

Elsherbiny

Amin

Aleem

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In this study however cultures of Phytophthora species, P palmivora and Phytophthora megakarya, were preserved successfully in SDW for 60 months (5 years). Sterile water medium has been used widely to store fungal cultures either at room temperature or at lower temperatures between 4°C and 5°C (Marx & Daniel, 1976;Hartung De Capriles et al, 1989;Borba Cde et al, 1992;Burdsall Jr and Dorworth, 1994;Ko, 2003;Borman et al, 2006;Elliot 2005;Sutton et al 2007, Roy et al, 2014Cui et al, 2018). Most of the studies have confirmed that keeping the cultures at room temperature is more appropriate for long term storage than lower temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Sterile water medium is an easy and cheaper way of preserving Phytophthora and other oomycete cultures for a longer period (Sutton et al 2007, Roy et al, 2014Cui et al, 2018). In this study, we modified the sterile water by replacing it with sterile soil suspension (SSS).…”

Section: Discussionmentioning

confidence: 99%

A simple method for the extension of shelf life of cultures of Phytophthora species causing black pod disease of cacao (Theobroma cacao L.)

Amoako-Attah

Kumi-Asare

Bukari

2021

gjs

View full text Add to dashboard Cite

Black pod disease of cacao caused by Phytophthora palmivora and Phytophthora megakarya in Ghana take heavy toll of cacao production in the field. Intensive research has been carried out worldwide on these pathogens. However, viability of the cultures during prolonged storage has remained a major challenge in the research. This paper reports findings of assessment of six storage media viz sterilised distilled water (SDW), sterilised and unsterilized soil suspension (SSS and USS), vegetable 8 juice broth (V8JB), Oat Meal Agar slant under mineral oil (at 4°C) and empty tube. Viability of the cultures was assessed on V8JA and in tetrazolium chloride test. Ability of zoospores of the cultures to infect cacao leaf discs was used to assess growth vigour and pathogenicity. Phytophthora cultures stored in SDW (26 ± 2°C; alternating day light and night) were preserved for 60 days (5 years). Both P. palmivora and P. megakarya performed better on SDW and SSS than on USS due to removal of staling substances in the soil medium by the sterilisation. Vigour of growth and pathogenicity of the stored cultures required re-inoculation of host tissue (cacao pod) in order to maintain potency to continually infect host.

show abstract

“…The long-term preservation protocols for pathogenic Peronosporomycetes mainly include cryopreservation, storage under mineral or paraffin oil, or storage in sterile water at room temperature [16]. However, the viability and/or genetic stability of these organisms are generally difficult to maintain for long periods of time [99]. Indeed, a number of marine Halophytophthora strains cannot be maintained in conditions of active growth for a long time, nor can they be preserved using standard long-term cryopreservation protocols.…”

Section: Case-study 6: Preservation Of Halophytophtora Spp (Peronospo...mentioning

confidence: 99%

Selected Case Studies on Fastidious Eukaryotic Microorganisms: Issues and Investigation Strategies

et al. 2023

View full text Add to dashboard Cite

The concept of fastidious microorganisms currently found in scientific literature is mainly related to the difficulty of isolating/culturing/preserving bacteria. Eukaryotes are investigated much less in this respect, although they represent a fundamental part of the microbial world. Furthermore, not only isolation, but also identification and culturing (in the perspective of long-term preservation) should be considered key aspects often impacting on the study of fastidious microorganisms, especially in terms of preservation in culture collections and biotechnological exploitation. The present review aimed to investigate the current state of the art on fastidious eukaryotes, with special emphasis on the efforts to improve their isolation, identification, culturing and long-term preservation in culture collections practices. A few case studies focused on some fastidious eukaryotic microorganisms (including possible customized solutions to overcome specific issues) are also presented: isolation and preservation of slow-growing fungi, culturing of Haematococcus lacustris, isolation of unialgal strains of Cyanidiophytina (Rhodophyta), identification of Metschnikowia pulcherrima clade yeasts, isolation and preservation of Pyricularia species, preservation of Halophytophtora spp.

show abstract

Simple and inexpensive long-term preservation methods for Phytophthora infestans

Cited by 8 publications

References 20 publications

Trichoderma Volatile Organic Compounds as a Biofumigation Tool against Late Blight Pathogen Phytophthora infestans in Postharvest Potato Tubers

Trichoderma Volatile Organic Compounds as a Biofumigation Tool against Late Blight Pathogen Phytophthora infestans in Postharvest Potato Tubers

A simple method for the extension of shelf life of cultures of Phytophthora species causing black pod disease of cacao (Theobroma cacao L.)

Selected Case Studies on Fastidious Eukaryotic Microorganisms: Issues and Investigation Strategies

Contact Info

Product

Resources

About