Simple and Inexpensive Water Extraction Method for Assaying Potassium Concentration in Tobacco Plant Tissue

Reddy, D. Damodar; Veeranki, Krishnamurthy

doi:10.1080/00103624.2012.747603

Cited by 5 publications

(7 citation statements)

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“…The sample measured range was calculated as 0.76–3.68% K based on the sample weight, dilution ratio of the sample and recoveries and the limit of quantification was 0.76%. These measured K values covered the major range of tobacco leaf tissue K contents (0.50–3.88%) reported in the literature . If few samples are below or above the range, the extract should be concentrated or diluted.…”

Section: Resultsmentioning

confidence: 77%

“…The results showed that the highest K extraction recovery was 98.9%, and the optimum conditions were a liquid-to-material ratio of 40.0, an extraction temperature of 100 °C, and an extraction time of 10.75 min. The extraction recovery depends on plant tissue resistance in the form of a physical barrier to K release . Heating extraction with high temperature can soften tobacco matrices and increase the solubility of K salt to improve extraction recovery .…”

Section: Resultsmentioning

confidence: 99%

“…The minerals and trace elements in plant tissue are usually determined following wet digestion or high temperature (450 °C) ashing techniques involving digestion with single acid, triacid, or diacid mixtures (HNO 3 , H 2 SO 4 , and HClO 4 ) or heating dissolution with HCl, but these methods are often costly, relatively dangerous, or time consuming due to the use of strong acid, large amounts of dry sample. , K in plant tissue is not bound to organic compounds and occurs in soluble forms, thus indicating the ease of its extractability . Recently, a nondigestion extraction method was proposed for the estimation of plant K content using solution extraction with water, acetic acid, CaCl 2 , salt EDTA, or ammonium/magnesium acetate .…”

Section: Resultsmentioning

confidence: 99%

“… 26 , 27 K in plant tissue is not bound to organic compounds and occurs in soluble forms, thus indicating the ease of its extractability. 27 Recently, a nondigestion extraction method was proposed for the estimation of plant K content using solution extraction with water, acetic acid, CaCl 2 , salt EDTA, or ammonium/magnesium acetate. 28 Compared with digestion and ashing techniques, more sample pretreatment can be handled in the same amount of time.…”

Section: Resultsmentioning

confidence: 99%

“…The extraction recovery depends on plant tissue resistance in the form of a physical barrier to K release. 27 Heating extraction with high temperature can soften tobacco matrices and increase the solubility of K salt to improve extraction recovery. 30 The optimum conditions were round off for simplicity of operation, and the optimum extraction conditions were 40, 100 °C, and 11 min.…”

Section: Resultsmentioning

confidence: 99%

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Development and Application of Portable Reflectometric Spectroscopy Combined with Solid-Phase Extraction for Determination of Potassium in Flue-Cured Tobacco Leaves

Cai

Gao

et al. 2023

ACS Omega

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Potassium (K) plays important roles in the energy and substance conversion of tobacco metabolism and is also regarded as one of the important indicators of tobacco quality evaluation. However, the K quantitative analytical method shows poor performance in terms of being easy-to-use, cost-effective, and portable. Here, we developed a rapid and simple method for the determination of K content in flue-cured tobacco leaves, including water extraction with 100 °C heating, purification with solid-phase extraction (SPE), and analysis with portable reflectometric spectroscopy based on K test strips. The method development consisted of optimization of the extraction and test strip reaction conditions, screening of SPE sorbent materials, and evaluation of the matrix effect. Under the optimum conditions, good linearity was observed in 0.20–0.90 mg/mL with a correlation coefficient >0.999. The extraction recoveries were found to be in the range of 98.0–99.5% with a repeatability and reproducibility of 1.15–1.98% and 2.04–3.26%, respectively. The sample measured range was calculated to be 0.76–3.68% K. Excellent agreement was found in accuracy between the developed reflectometric spectroscopy method and the standard method. The developed method was applied to analyze the K content in different cultivars, and the content varied greatly among the samples with lowest and highest contents for Y28 and Guiyan 5 cultivars, respectively. This study can provide a reliable approach for K analysis, which may become available on-site in a quick on-farm test.

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Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%