Simple and rapid formic acid sample treatment for the isolation of HgSe nanoparticles from animal tissues

Hanafi, Khouloud El; Gomez-Gomez, Beatríz; Pedrero, Zoyne; Bustamante, Paco; Cherel, Yves; Amouroux, David; Madrid, Yolanda

doi:10.1016/j.aca.2023.340952

Cited by 5 publications

(3 citation statements)

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“…The resulting extract was filtrated using 50 kDa cutoff PTFE filters (Amicon Ultra) and abundantly washed with ultrapure water until total removal of soluble Hg and Se was achieved. Nanoparticles were then recovered by centrifugation for 3 min at 1000 g …”

Section: Methodsmentioning

confidence: 99%

“…Both Se species-specific fraction isolations were carried out as described elsewhere. ,, Briefly, fresh samples (conserved at −80 °C) [0.1–0.3 g wet weight (ww)] were homogenized and diluted in 3 mL of ultrapure water. Aqueous extraction was performed using ultrasonication (30 s at 21% of 100 W), followed by centrifugation at 14.5 rpm for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…Selenium speciation in wild birds has been rarely reported. Recent studies in apex predator seabirds, specifically giant petrels, revealed the presence of mercury selenide nanoparticles (HgSe NPs) , and selenoneine in several organs . HgSe NPs are considered to be a final product of methylmercury (MeHg) demethylation, and they have been identified in the liver, kidney, muscle, and brain tissue .…”

Section: Introductionmentioning

confidence: 99%

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First-Time Isotopic Characterization of Seleno-Compounds in Biota: A Pilot Study of Selenium Isotopic Composition in Top Predator Seabirds

Marchán-Moreno,

Louvat,

Bueno

et al. 2024

Environ. Sci. Technol.

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This study pioneers the reporting of Se isotopes in marine top predators and represents the most extensive Se isotopic characterization in animals to date. A methodology based on hydride generation�multicollector inductively coupled plasma mass spectrometry�was established for such samples. The study was conducted on various internal organs of giant petrels (Macronectes spp.), encompassing bulk tissues (δ 82/78 Se bulk ), distinct Se-specific fractions such as selenoneine (δ 82/78 Se SEN ), and HgSe nanoparticles (δ 82/78 Se NPs ). The δ 82/78 Se bulk results (2.0−5.6‰) offer preliminary insights into the fate of Se in key internal organs of seabirds, including the liver, the kidneys, the muscle, and the brain. Notably, the liver of all individuals was enriched in heavier Se isotopes compared to other examined tissues. In nanoparticle fraction, δ 82/78 Se varies significantly across individuals (δ 82/78 Se NPs from 0.6 to 5.7‰, n = 8), whereas it exhibits remarkable consistency among tissues and individuals for selenoneine (δ 82/78 Se SEN , 1.7 ± 0.3‰, n = 8). Significantly, there was a positive correlation between the shift from δ 82/78 Se bulk to δ 82/78 Se SEN and the proportion of Se present as selenoneine in the internal organs. This pilot study proves that Se species-specific isotopic composition is a promising tool for a better understanding of Se species fate, sources, and dynamics in animals.

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Section: Methodsmentioning

confidence: 99%