Simple and rapid HPLC method for simultaneous determination of atenolol and chlorthalidone in spiked human plasma

Abstract

A simple, sensitive and rapid chromatographic method was developed and validated for the simultaneous quantification of atenolol and chlorthalidone in human plasma using hydrochlorothiazide as internal standard (IS). The method utilized proteins precipitation with acetonitril as the only sample preparation involved prior to reverse phase-HPLC. The analytes were chromatographed on Shim-pack cyanopropyl column with isocratic elution with 10 mM KH2PO4 (pH 6.0) - methanol (70:30, v/v) at ambient temperature with flow rate of 1 mL min(-1) and UV detection at 225 nm. The chromatographic run time was less than 10 min for the mixture. The calibration curves were linear over the range of 0.1-10 μg mL(-1). The method was validated in terms of accuracy, precision, absolute recovery, freeze-thaw stability, bench-top stability and re-injection reproducibility. The within- and between-day accuracy and precision were found to be within acceptable limits <15%. The analytes were stable after three freeze-thaw cycles (deviation <15%). The proposed method was specific for the simultaneous determination of atenolol and chlorthalidone in human plasma where there was no interference from endogenous biological substances.

Development and Validation of Stability Indicating Gradient Rp-HPLC Method for Simultaneous Estimation of Telmisartan and Chlorthalidone in Bulk Api and Fixed Dose Combination

“…[3][4][5] Literature review reveals that HPLC. [21][22][23][24] UV [25] spectrophotometric methods have been reported for quantification of CHLO in pharmaceutical dosage form. Literature review reveals that HPTLC [26] HPLC [27][28][29][30] methods have been reported for simultaneous estimation of TEL and CHLO in dosage forms however, so far, no method was reported qualitative estimation of any degradation product with the simultaneous determination of Telmisartan (TEL) and Chlorthalidone (CHLO) in bulk API and FDC.…”

Chemometric resolution of coeluting peaks of eleven antihypertensives from multiple classes in high performance liquid chromatography: A comprehensive research in human serum, health product and Chinese patent medicine samples

“…Nowadays, health products are widely adopted as supplementary antihypertensive strategy, while the adulteration product has been an emerging issue of concern to the public health. To a patient undergoing therapeutic treatment, misuse of adulterated health product may result in unexpected changes in the blood pressure and might cause harmful effects to patients [2,11]. Consequently, monitoring the qualities of health product in the market is a work of great importance.…”

“…In principle, the joint of complementary medicines can not only help to meet the target blood pressure, but also decrease the morbidity and mortality of complications. In practice, many doctors as well as the World Health Organization have been beginning to detector (UV) [10][11][12][13], fluorescence detector (FD) [14,15], amperometric detector (AD) [16], diode array detector (DAD) [17][18][19] and mass spectrometry (MS) [20][21][22]. However, most works have been focused on quantifying a small amount or one kind of antihypertensives in biological matrix or Chinese herbal matrix.…”

Direct Spectrophotometric Determination of Atenolol and Timolol Anti-Hypertensive Drugs

“…Several analytical methods have been reported for the determination of atenolol in human plasma, urine, or pharmaceutical preparations, such as high-performance liquid chromatography [5][6][7][8][9], gas chromatography [10], liquid chromatography [11,12]. Other techniques include voltammetric [13][14][15], electrophoresis [16,17], chemiluminescence [18][19][20], spectrofluorimetric [21], ultraviolet and visible spectrophotometry [22][23][24][25][26][27].…”