Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection

Hu, Wei; Zhang, Yun; Yang, Hang; Yu, Junping; Wei, Hongping

doi:10.1016/j.mimet.2015.04.018

Cited by 7 publications

(8 citation statements)

References 17 publications

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“…coli O157:H7 detection, they require expensive equipment and are, therefore, not widely applicable for on-site detection. Given these limitations, naked-eye biosensors that rely on the use of a homogeneous colorimetric assay and lateral flow immunoassay , have been explored for their potential suitability for the low-cost and convenient detection of E. coli O157:H7.…”

Section: Introductionmentioning

confidence: 99%

“…22 Although these methods are capable of highly sensitive and selective E. coli O157:H7 detection, they require expensive equipment and are, therefore, not widely applicable for on-site detection. Given these limitations, naked-eye biosensors that rely on the use of a homogeneous colorimetric assay 23 and lateral flow immunoassay 24,25 have been explored for their potential suitability for the low-cost and convenient detection of E. coli O157:H7. However, the relatively low sensitivity of these methods currently limits their use for on-site testing.…”

Section: ■ Introductionmentioning

confidence: 99%

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Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification

Kim

Mun

et al. 2018

J. Agric. Food Chem.

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Here, we report an enhanced colorimetric method using enzymatic amplification with nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) precipitation for the ultrasensitive detection of Escherichia coli O157:H7 through immunomagnetic separation-selective filtration. Biotinylated anti- E. coli O157:H7 antibody and streptavidin-alkaline phosphatase were conjugated to the surface of magnetic nanoparticles, and E. coli O157:H7-conjugates complexes remained on the membrane filter surface. The resultant light brown spots on the membrane filter were amplified with NBT/BCIP solution to yield enzyme-catalyzed precipitation, which increased with an increasing E. coli O157:H7 concentration. E. coli O157:H7 was detected in pure samples with limits of detection of 10 and 6.998 colony-forming units (CFU)/mL through visual observation and measurement of optical density, respectively. The proposed method was applied to a lettuce sample inoculated with selective E. coli O157:H7, which was detected within 55 min without cross-reactivity to non-target bacteria. This enhanced colorimetric method has potential for on-site detection of food contaminants and environmental pollutants.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification

Kim

Mun

et al. 2018

J. Agric. Food Chem.

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“…Toward this end, we first prepared a novel monoclonal antibody (mAb) of GCA. Because the Fc fragment of this anti-GCA antibody could naturally bind to protein A on a previously reported red-colored bacteria S. aureus, 30 functionalized bacterial bioprobe (Scheme 1). We found that the addition of the bioprobes into a drop of GCA solution on a glass slide could lead to the aggregation of the bioprobes into a red spot on the slide, allowing us to rapidly and visually detect the GCA with high specificity and sensitivity.…”

Section: ■ Introductionmentioning

confidence: 99%

Rapid Naked-Eye Detection of a Liver Disease Biomarker by Discovering Its Monoclonal Antibody to Functionalize Engineered Red-Colored Bacteria Probes

Shen

Zhao

et al. 2021

ACS Omega

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Glycocholic acid (GCA) is a biomarker for liver diseases, but few facile naked-eye detection methods have been reported to detect it till now. To tackle this challenge, we first prepared a novel monoclonal mouse antibody (mAb) of GCA by a hybridoma technique. The anti-GCA mAb exhibited high specificity, making its cross-reactivity with seven structurally and functionally related GCA analogs negligible. Using this anti-GCA mAb and an engineered red-colored bacterial strain (Staphylococcus aureus, S. aureus), we developed a simple naked-eye visualized method for GCA detection. Toward this goal, S. aureus bacteria were turned red by 5-cyano-2,3-ditolyl tetrazolium chloride treatment and heat treated to an unculturable state, rendering the bacteria as an optical detection probe powerful in in vitro diagnostics. Through the natural binding ability of protein A on the surface of S. aureus and the Fc fragment of a mouse antibody, the anti-GCA antibody was simply conjugated onto S. aureus. Then, the engineered S. aureus served as a red-colored bioprobe for detecting GCA through a coagglutination test. In the presence of GCA, the bioprobes aggregated into dense red-colored eye-visible clusters, enabling the sensitive detection of GCA with a concentration of 0.05−0.10 μg/mL. This naked-eye visualization method only takes a few minutes to detect GCA and avoids the use of expensive equipment. It represents a rapid, convenient, and simple method for detecting GCA to diagnose liver diseases.

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“…Primarily, Staphylococcal protein A (SPA) was used as an ideal detection marker of S. aureus, since SPA is only expressed in the cell wall of 99% S. aureus isolates and the number of SPA molecules is estimated to approximately 80 000 in each bacterium. [16][17][18][19] Dog IgG was utilized as the capture antibody that binds to S. aureus based on the strong affinity between the Fc region of dog IgG and SPA. Since dog IgG possesses strong binding capacity towards protein A and low binding capacity towards protein G, 20,21 it can eliminate the interference from protein G producing Streptococcus to a certain extent.…”

Section: Introductionmentioning

confidence: 99%

A novel colorimetric sensing platform for the detection ofS. aureuswith high sensitivity and specificity

et al. 2019

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Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection

Cited by 7 publications

References 17 publications

Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification

Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification

Rapid Naked-Eye Detection of a Liver Disease Biomarker by Discovering Its Monoclonal Antibody to Functionalize Engineered Red-Colored Bacteria Probes

A novel colorimetric sensing platform for the detection ofS. aureuswith high sensitivity and specificity

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