Simple approach for ultrasensitive electrochemical immunoassay of Clostridium difficile toxin B detection

Fang, Yishan; Chen, Shu‐Yi; Huang, Xinjian; Wang, Lishi; Wang, Haiying; Wang, Jufang

doi:10.1016/j.bios.2013.09.063

Cited by 44 publications

(17 citation statements)

References 45 publications

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“…A simple method for the determination of TcdB was developed by means of a sandwich-type electrochemical immunosensor. Greatly enhanced sensitivity was achieved (LOD of 0.7 pg·mL −1 ) by fabricating the immunosensor using a layer-by-layer coated MWCNTs, PB, Chit and glutaraldehyde composite on the working electrode as well as HRP-graphene oxide as a nanocarrier in a multienzyme amplification strategy [68]. Moreover, this sensitive immunosensor provided satisfactory results in the analysis of real human stool samples.…”

Section: Graphene Used As Carrier For Signal Elements In Electrochemimentioning

confidence: 96%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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Section: Graphene Used As Carrier For Signal Elements In Electrochemimentioning

confidence: 96%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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“…In the last two years, some immunoassays have been developed [116][117][118][119] that can overcome the stagnation of C. difficile toxin detection. These methods, are in many cases simples, fast and ultrasensitive, and have a large potential for clinical applications in the future.…”

Section: Difficile Toxinsmentioning

confidence: 99%

Rapid Tests for Detection of Main Clostridial Toxins

Pérez-Etcheverry

Carmen

2017

IRA-JAS

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“…Alternative immunoassay approaches to ultrasensitive toxin detection are currently in early development, including two based on sandwich-type electrochemical immunosensor methodology (43,44). Preliminarily, both methods appear to be able to detect toxins in buffer with analytical LODs of Յ1 pg/ml; definition of LOD for toxin in stool samples and, ultimately, assay validation using well-characterized clinical samples will provide important detail as to the potential clinical utility of these approaches.…”

Section: Novel Approaches To Ultrasensitive Toxin Detectionmentioning

confidence: 99%

Ultrasensitive Detection and Quantification of Toxins for Optimized Diagnosis of Clostridium difficile Infection

Pollock

2016

J Clin Microbiol

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Recently developed ultrasensitive and quantitative methods for detection ofClostridium difficiletoxins provide new tools for diagnosis and, potentially, for management ofC. difficileinfection (CDI). Compared to methods that detect toxigenic organism, ultrasensitive toxin detection may allow diagnosis of CDI with increased clinical specificity, without sacrificing clinical sensitivity; measurement of toxin levels may also provide information relevant to disease prognosis. This minireview provides an overview of these new toxin detection technologies and considers what these new tools might add to the field.

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Simple approach for ultrasensitive electrochemical immunoassay of Clostridium difficile toxin B detection

Cited by 44 publications

References 45 publications

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Rapid Tests for Detection of Main Clostridial Toxins

Ultrasensitive Detection and Quantification of Toxins for Optimized Diagnosis of Clostridium difficile Infection

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