Simple assay of 0.1–1.0 pmol of ATP, ADP, and AMP in single somatic cells using purified luciferin luciferase

Spielmann, Horst; Jacob-Müller, Ursula; Schulz, Petra

doi:10.1016/0003-2697(81)90061-0

Cited by 115 publications

(39 citation statements)

References 11 publications

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“…The concentrations of adenine nucleotides (ATP, ADP and AMP) were determined using the luciferin\luciferase system to quantify ATP (Speilmann et al, 1982). Samples (20 ml) from the fermenter were rapidly quenched ( 0n5 s) by collection in sterile Universal tubes containing 4 ml conc.…”

Section: Introductionmentioning

confidence: 99%

Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina

Wynn¹,

Hamid²,

Li³

et al. 2001

Microbiology

173

110

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The biochemical events associated with the onset of lipid accumulation in Mucor circinelloides and Mortierella alpina, under conditions of nitrogenlimited growth, have been elucidated ; they differ in key aspects from those described in oleaginous yeasts. The NAD M :isocitrate dehydrogenases of Mc. circinelloides and Mort. alpina were not absolutely dependent on AMP for activity. Furthermore, changes in the cellular adenine nucleotide pools and energy charge were different from those reported for oleaginous yeasts. In Mc. circinelloides ATP, ADP and AMP concentrations all decreased by 50 % after nitrogen limitation, leading to a constant energy charge at the expense of the size of the total adenylate pool. Pyruvate carboxylase in Mc. circinelloides was cytosolic, having implications for the organization of lipid synthesis in filamentous fungi. As a result of the data obtained, a revised and more concerted mechanism for the initiation of storage lipid accumulation is put forward for filamentous fungi.

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Section: Introductionmentioning

confidence: 99%

Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina

Wynn¹,

Hamid²,

Li³

et al. 2001

Microbiology

173

110

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“…There was, however, some evidence of greater responses for example in viability Follicles were prepared as from Table 4; n 5 3 sheep and 4 to 6 replicates of each containing 4 follicles. ATP was analysed by the method of Spielmann et al (1981). Data analysed by one-way ANOVA values in the same row with dissimilar subscripts are different (P , 0.01) (Adapted from Tahmasbi et al, 2007a).…”

Section: [U-mentioning

confidence: 99%

In vitro methodology, hormonal and nutritional effects and fibre production in isolated ovine and caprine anagen hair follicles

Galbraith

2010

Animal

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Mammalian hair follicles are complex multicellular structures in the skin, which produce hair fibre under the influence of locally produced and systemic signalling systems. Investigation to determine mechanisms of regulation, follicular responses and the importance of nutritional supply have utilised a number of in vivo and in vitro approaches. Included in these are studies on isolated intact anagen secondary follicles singly or in groups with incubation in culture medium. These utilise techniques developed for investigation of follicles from human skin. Results from selected studies reviewed here demonstrate differences in capacity for hair growth and protein synthesis between secondary follicles from Angora and cashmere-bearing goats. Mohair follicles were shown to exhibit faster hair shaft elongation both in vivo and in vitro, to have greater DNA content per follicle and to deposit significantly more protein per follicle and per unit of DNA. Incubation of anagen mohair and cashmere follicles in the presence of melatonin or prolactin showed positive responses in hair shaft growth and protein synthesis to both signalling molecules. This result indicated directly acting effects on the follicle in addition to any indirect effects arising at a whole animal level in response to, for example, variation in photoperiod. Similarly, epidermal growth factor was shown to alter elongation and protein synthesis in mohair follicles and to produce, at higher concentration, club hair structures similar to effects observed in other species. The vitamin biotin was shown to be important in maintaining viability of isolated sheep secondary hair follicles where supplementation increased the proportion continuing to grow. Effects on growth and apparent protein synthesis suggested comparatively lesser effects on follicles, which remained viable. Histology on follicles indicated effects of biotin deficiency in reducing proliferation of basal keratinocytes. The final study, included in this review, demonstrated that supply of the essential sulphur-containing amino acid L-methionine was necessary to maintain the viability and growth of mohair follicles. L-cysteine was not required in the presence of L-methionine, although there was evidence of an optimisation when both amino acids were present in adequate concentrations. Consideration is given to the importance of transport mechanisms and capacity to utilise absorbed nutrients when considering optimising nutritional supply to individual follicles. These may then provide targets for attainment in applied nutrition of animals in vivo.Keywords: hair follicle, in vitro culture, cashmere, mohair, sheep wool ImplicationsHair fibres are products with value depending on quality and quantity produced. We require information to optimise breeding and husbandry of fibre-producing animals. This includes better understanding of the hair follicles in skin, which produce fibre. This report reviews results from in vitro studies on follicles removed from skin of sheep and goats. It describes how: (i) var...

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“…In the cyclic method, several sets of experiments were performed in which the concentration of adenylate kinase was varied in the range 0.096-1.92 units, while the pyruvate kinase concentration was kept constant, and vice versa (pyruvate kinase being varied in the range 0.098-1.96 units while adenylate kinase was kept constant), in the presence of ATP or ADP (Figure 3). The values of λ thus obtained for each set of experiments were fitted by non-linear regression to eqn (11), after a preliminary check of linearity between the activity expressed and the amount of enzyme for the two kinases involved in the cycle (as explained in the Experimental section), taking for…”

Section: Determination Of K 1 and Kmentioning

confidence: 99%

“…Another possibility is the replacement of hexokinase by adenylate kinase (EC 2.7.4.3) in the presence of AMP [11,12]. The kinetics of the system in this case is complex because two molecules of ADP are generated at each turn of the cycle from one of ATP and one of AMP.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of a self-amplifying substrate cycle: ADP‒ATP cycling assay

Valero¹,

Varón

García-Carmona

2000

Biochem. J.

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A kinetic study of an ATP-ADP amplification cyclic system involving the enzymes adenylate kinase, pyruvate kinase and -lactate dehydrogenase has been made. The stoichiometry of the cycle is 2 : 1, because two molecules of ADP are synthesized from one each of ATP and AMP, and one molecule of ADP is converted back into one of ATP at each turn of the cycle. This results in a continuous exponential increase in the concentrations of ATP and ADP in the reaction medium, according to the equations obtained. This is therefore a substrate cycle that

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Simple assay of 0.1–1.0 pmol of ATP, ADP, and AMP in single somatic cells using purified luciferin luciferase

Cited by 115 publications

References 11 publications

Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina

Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina

In vitro methodology, hormonal and nutritional effects and fibre production in isolated ovine and caprine anagen hair follicles

Kinetics of a self-amplifying substrate cycle: ADP‒ATP cycling assay

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