Simple Automated Determination of Serum or Plasma Glucose by a Hexokinase/Glucose-6-Phosphate Dehydrogenase Method

Neeley, William E.

doi:10.1093/clinchem/18.6.509

Cited by 122 publications

(42 citation statements)

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“…A diagnostic work up for all patients was followed that included; complete blood picture, (18) measurement of liver enzymes including serum levels of alanine and aspartate aminotransferases (ALT and AST respectively), (19) assessment of renal function tests: urea and creatinine serum levels, (20) assessment of fasting blood glucose level, (21) and erythrocyte sedimentation rate (ESR) after first and second hours. For Mantoux tuberculin skin test, 0.1 ml purified protein derivative (PPD) containing 5 tuberculin units was injected intradermally at the mid volar aspect of the left forearm.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis

Eldeeb

Malwany

Khalil

et al. 2014

Journal of High Institute of Public Health

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Background: Tuberculosis is one of the deadliest diseases over the world. Diagnosis of pulmonary TB depends on combination various parameters. IGRA measure T cell release of IFN-γ in response to M.TB antigen. WHO policy statement as on IGRA use in low and middle income countries is not established yet. Objective: The present study aimed to compare between tuberculin skin test (TST) and interferon gamma release assay (IGRA) in the diagnosis of active tuberculosis infection and study the effect of 3 months of first line anti-TB therapy on the positivity of the IGRA test. Methods: 40 Egyptian patients were included in the study, and assigned as two groups; Group I comprised 20 patients with negative sputum for AFB by Ziehl-Neelsen stains with positive sputum culture for M.TB and Group II included 20 patients with positive sputum and Ziehl-Neelsen for AFB before and after 3 months of first line of anti-TB therapy. All patients were subjected to full history taking, clinical examination, X-ray chest, lab investigations, ESR measurements, microbiological tests and ELISA measurement of Quantiferon-TB Gold. Results: Lower significant values were found in group II after treatment than before treatment regarding clinical parameters and 1 st and 2 nd hours ESR. IGRA test and TST showed sensitivity (91.18%, 76.4%), specificity (83.33%, 66.67%), positive predictive value (96.88%, 92.86%), negative predictive value (62.5%, 33.3%) and accuracy of (90%, 75%) respectively. IGRA results had no statistical significant differences between the studied groups with poor agreement with TST ((κ) = 0.025). Conclusion: IGRAS test had high sensitivity and specificity in diagnosis of active TB. More studies are needed to evaluate the effect of anti-TB therapy on IGRA level.

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Section: Laboratory Investigationsmentioning

confidence: 99%

Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis

Eldeeb

Malwany

Khalil

et al. 2014

Journal of High Institute of Public Health

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“…HbA1c was measured using a 765 Glycomat analyzer employing low-pressure cation exchange chromatography in conjunction with gradient elution to separate hemoglobin subtypes and was associated with a coefficient of variation o4%. Total cholesterol and glucose were measured using enzymatic methods, 16,17 LDL cholesterol was calculated, 18 and HDLcholesterol was measured after precipitation of vLDL and LDL fractions. 19 Statistical methods All analyses were performed using SPSS Version 11.1 and Microsoft Excel Version 9.0.…”

Section: Collection and Storage Of Blood Samplesmentioning

confidence: 99%

Ethnic differences in the relationships between obesity and glucose-metabolic abnormalities: a cross-sectional population-based study

et al. 2005

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“…Serum insulin levels were measured by immunoradiometric assay (IRMA; Biosource Technologies, Inc., Fleurus, Belgium), glucose levels by the hexokinase method (Gluco-quant, Roche Molecular Biochemicals, Mannheim, Germany) (30), and free IGF-I levels by a two-site IRMA (Active, Diagnostic Systems Laboratories, Inc., Webster, TX) direct assay. Serum was incubated in tubes and decanted, and the tubes were washed according to the manufacturer's instructions.…”

Section: Hormone Assaysmentioning

confidence: 99%

Free Androgen Index and Leptin Are the Most Prominent Endocrine Predictors of Ovarian Response during Clomiphene Citrate Induction of Ovulation in Normogonadotropic Oligoamenorrheic Infertility

Imani¹

2000

Journal of Clinical Endocrinology & Metabolism

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We have previously demonstrated that obese hyperandrogenic amenorrheic women are less likely to ovulate after clomiphene citrate (CC) medication. The present study was designed to identify whether additional endocrine screening characteristics, all potentially involved in ovarian dysfunction in 182 normogonadotropic oligoamenorrheic infertile women, are associated with ovarian response, which may improve overall prediction of CC-resistant anovulation. Standardized endocrine screening took place before initiation of CC medication (50 mg/day; increasing doses up to 150 mg/day if required) from cycle days 3-7. Screening included serum assays for fasting insulin and glucose, insulin-like growth factor I (IGF-I), IGF-binding protein-1 (IGFBP-1), IGFBP-3, free IGF-I, inhibin B, leptin, and vascular endothelial growth factor. Forty-two women (22% of the total group) did not ovulate at the end of follow-up (a total number of 325 cycles were analyzed). Fasting serum insulin, insulin/glucose ratio, IGFBP-1, and leptin were all significantly different in univariate analyses (P < or = 0.02), comparing CC responders vs. nonresponders. Forward stepwise multivariate analyses in combination with factors reported earlier for prediction of patients remaining anovulatory after CC revealed a prediction model including 1) free androgen index (FAI = testosterone/sex hormone-binding globulin ratio), 2) cycle history (oligomenorrhea or amenorrhea), 3) leptin level, and 4) mean ovarian volume. These data suggest that decreased insulin sensitivity, hyperandrogenemia, and obesity, all associated with polycystic ovary syndrome, are prominent factors involved in ovarian dysfunction, preventing these ovaries from responding to stimulation by raised endogenous FSH levels due to CC medication. By using leptin instead of body mass index or waist to hip ratio, the previous model for prediction of patients remaining anovulatory after CC medication could be slightly improved (area under the curve from 0.82-0.85). This may indicate that leptin is more directly involved in ovarian dysfunction in these patients. The capability of insulin and IGFBP-1 to predict patients who remain anovulatory after CC disappears when FAI enters into the model due to a significant correlation between FAI and these endocrine parameters. This suggests that markers for insulin sensitivity (e.g. IGFBP-1 and insulin) are associated with abnormal ovarian function through its correlation with androgens, whereas leptin is directly involved in ovarian dysfunction.

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Simple Automated Determination of Serum or Plasma Glucose by a Hexokinase/Glucose-6-Phosphate Dehydrogenase Method

Cited by 122 publications

References 0 publications

Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis

Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis

Ethnic differences in the relationships between obesity and glucose-metabolic abnormalities: a cross-sectional population-based study

Free Androgen Index and Leptin Are the Most Prominent Endocrine Predictors of Ovarian Response during Clomiphene Citrate Induction of Ovulation in Normogonadotropic Oligoamenorrheic Infertility

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