Simple Bone Cyst

Fibrous Tissue of Subchondral Cystic Lesions in Horses Produce Local Mediators and Neutral Metalloproteinases and Cause Bone Resorption in Vitro

“…21,24 Of the variables measured, PGE2 concentrations of SCL were high compared with normal joints and severe OA joints. Our findings are consistent with observations of elevated PGE2 concentrations in UCB cyst fluid, 12,13,34 in vitro studies of tissue originating from human dental cysts 16,35 and in experimentally induced periapical cysts in rats. 36 Culture supernatants from dental cysts 15 and UCB 34 stimulated bone resorption in vitro.…”

“…Our findings are consistent with observations of elevated PGE2 concentrations in UCB cyst fluid, 12,13,34 in vitro studies of tissue originating from human dental cysts 16,35 and in experimentally induced periapical cysts in rats 36 . Culture supernatants from dental cysts 15 and UCB 34 stimulated bone resorption in vitro. PGE2 production by fibroblasts, collagenase synthesis in monolayer cultures of chondrocytes and fibroblasts, and fibroblast and osteoblast proliferation, were increased after exposure to conditioned media of dental cysts when compared with control tissue.…”

“…Decreased intraosseous pressure was believed to be responsible for this beneficial effect, but surgical drainage may have decreased the local concentrations of PGE2 and other substances responsible for bone resorption. Bone resorption in cultured 45 Ca‐labeled mouse ulnae was increased 2‐fold after incubation with UCB fluid collected during surgical drainage 34 . These results were very similar to those we obtained with the resorption pit assay in which a 2−fold increase of TRAP+ cells and an increase in the formation of resorption pits with the 1:2 dilution of conditioned media samples from cyst C was observed.…”

“…Percutaneous drilling and drainage has been reported to be an effective treatment of UCB 34,37 . Decreased intraosseous pressure was believed to be responsible for this beneficial effect, but surgical drainage may have decreased the local concentrations of PGE2 and other substances responsible for bone resorption.…”

“…Monocyte/macrophage cell infiltrate, stromal fibroblasts, and epithelial cells lining dental cysts have been reported to be the cellular origin of PGE2, MMPs, and cytokines 15,16,38,40 . Gelatinolytic activity was reported for UCB cyst fluid, but the cellular origin of the gelatinase was not identified 34 . Similar to SCL, UCB lesions are mainly composed of fibroblasts and small blood vessels 4 .…”