Simple colorimetric method to determine the in vitro antioxidant activity of different monoterpenes

Noacco, Nehuen; Rodenak-Kladniew, Boris; Bravo, Margarita María García de; Castro, Guillermo R.; Islan, Germán Abel

doi:10.1016/j.ab.2018.06.007

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…In general, detox enzymes (i.e., superoxide dismutase, catalase) are triggered to prevent oxidative reactions and several nonenzymatic antioxidants play a key role in the maintenance of homeostasis. The chemical structure of monoterpenes provides double bonds and reduced functional groups that are susceptible to oxidation and have indicated antioxidant features [14].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Oxidative Stress-Protective Effects of Five Monoterpenes from Softwood

et al. 2022

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Volatile organic compounds (VOC) affect the quality of indoor air. Terpenes and especially monoterpenes are the main molecules emitted from softwood material (coniferous species), which is widely used in construction. The corneal epithelium is one of the first human membranes to encounter VOCs in the air. Moreover, the industrial use of pleasant-scented monoterpenes in cosmetics, food, and detergents exposes people to monoterpenes in their daily lives. In the present study, the health effective properties of five monoterpenes from softwood were tested; cytotoxicity and oxidative stress-protective effects of α- and β-pinenes, R- and S-limonene, and 3-carene were tested in a human corneal epithelial (HCE) cell model system and with two additional in vitro antioxidant tests: oxygen radical absorbance capacity (ORAC) and hydrogen peroxide (H2O2) scavenging. Antibacterial efficacies were tested with two bioluminescent bacterial biosensor strains (Escherichia coli K12+pcGLS11 and Staphylococcus aureus RN4220+pAT19) and with minimum inhibitory concentration (MIC) test against Escherichia coli. Only very high concentrations of monoterpenes (0.3–0.5 mg/mL) demonstrated cytotoxicity against HCE cells. Contrary to the original hypothesis, monoterpenes did not exhibit strong antioxidant properties in tested concentrations. However, biosensors and MIC tests indicated clear antibacterial activities for all tested monoterpenes.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Oxidative Stress-Protective Effects of Five Monoterpenes from Softwood

et al. 2022

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“…Such result was supported by other authors (Rabbani et al, 2005). and antioxidant capacity were reported for geraniol, β-myrcene and linalool (Jayaprakasha et al, 2012;Bentayeb et al, 2014;Noacco et al, 2018). Also, linalool possesses strong antitumorigenic potential in 180 solid tumor sarcoma model in vivo which is accompanied by modulation of oxidative stress (Jana et al, 2014).…”

Section: Discussionmentioning

confidence: 95%

Cymbopogon citratus essential oil has hepato/renal protection and anti-genotoxicity against carbon tetrachloride

et al. 2020

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Nowadays, there is an increasing trend to use aromatherapy for treatment of various diseases. Cymbopogon citratus (Lemongrass) is one of many plants which have been reported to employ successfully in aromatherapy. Herein we decided to explore the protective role of Cymbopogon citratus essential oil (CCEO) against hepato/renal damage and genotoxicity induced by carbon tetrachloride (CCl4) and the relation of this bioactivity with its chemical constituents. Six main groups of mice (five/each) were examined: I- represents negative control group, II and III- mice received oral treatment with CCl4 (1mL/kg, positive control) and CCEO (0.3 mL/kg, control plant) respectively for five consecutive days and IV-VI- represent groups of mice treated with CCEO at the three concentrations 0.1, 0.2, 0.3 mL/kg plus CCl4 (five consecutive days treatment). Remarkable adverse effects of CCl4 in all the tested parameters were recorded. These effects were distinguished as an increment in the level of all liver marker enzymes (ALT, AST, ALP, γ-GT), blood urea, and creatinine. Also the oxidative stress biomarkers: malondialdehyde (MDA) and glutathione-transferase GST were affected after CCl4 treatment. Regarding to the genotoxic effect of CCl4, the percentage of chromosomal aberrations in bone marrow and spermatocyte cells was elevated (p< 0.05) compared with the negative control. Notable antioxidant, hepato/renal protection and anti-mutagenic potency of CCEO against CCl4 were demonstrated with a dose-related relationship. GC/MS analysis demonstrated the presence of 12 phytochemical constituents which in combination play a critical role in its antioxidant/antigenotoxic efficacy. The major components exist were E. Citral (35.13%) and Geraniol (32.83%).

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“…The two parameters are expressed using the following equations:LOD = 3 × σ / S LOQ = 10 × σ / S where, σ and S represent the standard deviation of the response and the slope of the curve, respectively. 25…”

Section: Methodsmentioning

confidence: 99%

“…Three analyte concentrations were evaluated: low concentration (close to the LOQ), medium concentration (about 2–3 fold higher than the LOQ), and high concentration (about 6- to 9-fold higher as compared to the LOQ). 25 RE (%) was calculated as:RE (%) = (measured value − real value)/real value × 100…”

Section: Methodsmentioning

confidence: 99%

Quantitative determination of voriconazole by thionine reduction and its potential application in a pharmaceutical and clinical setting

et al. 2023

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Simple colorimetric method to determine the in vitro antioxidant activity of different monoterpenes

Cited by 14 publications

References 31 publications

Antibacterial and Oxidative Stress-Protective Effects of Five Monoterpenes from Softwood

Antibacterial and Oxidative Stress-Protective Effects of Five Monoterpenes from Softwood

Cymbopogon citratus essential oil has hepato/renal protection and anti-genotoxicity against carbon tetrachloride

Quantitative determination of voriconazole by thionine reduction and its potential application in a pharmaceutical and clinical setting

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