Simple column‐switching HPLC method for determining levels of the antifungal agent micafungin in human plasma and application to patient samples

Abstract

Micafungin is an echinocandin antifungal agent that is important for treating candidiasis in emergency and intensive care medicine; however, current methods for measuring micafungin plasma levels are lengthy and complicated. We report a simple quantitative method using column-switching high-performance liquid chromatography (HPLC) for determining micafungin in human plasma samples. Human plasma was directly injected into a column-switching HPLC system with a MAYI-ODS pre-column to remove the plasma matrix. The calibration curve for micafungin showed good linearity in the range 0.1-10 µg/mL in human plasma. The mean relative standard deviation value of the intra-day and inter-day precision was less than 7.3%. More than 450 successive, accurate measurements were made before the system had to be washed with ammonium acetate solution. The therapeutic micafungin level in patients' plasma was successfully measured using this method. Because the pretreatment is simple and reproducible, our method can be used for routine therapeutic monitoring.

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

“…The pairing of these two characteristics allows SEC selection using the semipermeable methoxycellulose coating along with the pores of the porous silica gel particles to effectively exclude unwanted matrix interferences . This MC‐ODS trap has been heavily used in method development and is the focus of a number of application studies .…”

Untitled

“…Few HPLC methods have been reported in the literature for quantification of Micafungin in plasma [10][11][12][13] . Shengsheng et al reported stability indicating HPLC method for determination of Micafungin and related substances 14 .…”

Quantitative Analysis of Chloramphenicol in Royal Jelly by Column-switching LC-MS/MS Using a Pretreatment Column with a Higher-pressure Capability

“…Among pretreatment columns, the methylcellulose-immobilized reversed-phase (MC-ODS) column showed excellent performance for drug or chemical analysis of biological samples [1][2][3][4][5][6][7][8] and compounds in the environment. 9 While small molecules, such as drugs and environmental contaminants, enter in and are retained on the reversed-phase inner surface of small pores (12 nm), large molecules, such as proteins are excluded.…”