Simple Determination of Herbicides in Rice Paddy Water by Immunoassay

Míyake, Shiro; Ishíi, Yasuo; Yamaguchi, Yuki; Ohde, Katsuya; Motoki, Minoru; Kawata, Mitsuyasu; Ito, Shigekazu; Yuasa, Yojiro; Ohkawa, Hideo

doi:10.1021/bk-2003-0853.ch007

Cited by 6 publications

(4 citation statements)

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“…Water samples were mixed with 900 µL of distilled water and stored at −18°C until analysis of the BSM concentrations. The BSM analysis was carried out using a competitive enzyme‐linked immunosorbent assay ( elisa ) (Ishii 2003; Miyake et al . 2003).…”

Section: Methodsmentioning

confidence: 99%

“…Water samples were mixed with 900 mL of distilled water and stored at -18∞C until analysis of the BSM concentrations. The BSM analysis was carried out using a competitive enzyme-linked immunosorbent assay (ELISA) (Ishii 2003;Miyake et al 2003). Water samples or BSM standard solutions were mixed with equal volumes of BSM hapten-enzyme conjugated solution, and 100 mL of each mixture was incubated for 1.0 h at 25∞C in microtiter plates with 96 wells coated with anti-BSM monoclonal antibody.…”

Section: Bensulfuron Methyl Analysismentioning

confidence: 99%

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Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl

Aida

Itoh

Ikeda

et al. 2004

Weed Biology and Management

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Paddy herbicides have the potential to cause adverse effects on non-target plants. Susceptibilities of some aquatic ferns ( Azolla japonica Franch. et Savat., Isoetes japonica A. Braun, Marsilea quadrifolia L. and Salvinia natans All.) and duckweeds ( Lemna minor L. and Spirodela polyrhiza Schleid.) to paddy herbicide bensulfuron methyl (BSM) were evaluated with a 20 day exposure experiment using 200 cm 2 pots. The BSM concentrations in the surface water of monitoring pots with no plants dissipated exponentially with half lives of 3.5 and 3.9 days at application rates of 15 and 150 g ha -1 , respectively. The BSM concentrations in the surface water 1 day after application in the culture pots were comparable among plant species, and were lower than those in the monitoring pots. Bensulfuron methyl reduced the plant growth in all species. I . japonica showed the lowest intrinsic relative growth rate (RGR) and the lowest susceptibility with an effective dose resulting in 50% growth inhibition (ED 50 ) of 21 g ha -1 . Except for I . japonica , the RGR of the duckweeds was similar to the ferns, and ED 50 for the duckweeds was higher than the ferns. ED 50 for Sa . natans , A . japonica and M . quadrifolia were 1.1, 1.8 and 1.2 g ha -1 , respectively, which were smaller than 1/20 of the recommended field dose (51-75 g ha -1 ) and ranged from 1/2 to 1/6 of ED 50 for L . minor and Sp . polyrhiza (6.5 and 3.2 g ha -1 , respectively). These results suggest that BSM application in paddy fields and its runoff in some localities is expected to have adverse effects on the growth of Sa . natans , A . japonica and M . quadrifolia .

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Section: Methodsmentioning

confidence: 99%

Section: Bensulfuron Methyl Analysismentioning

confidence: 99%

Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl

Aida

Itoh

Ikeda

et al. 2004

Weed Biology and Management

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“…Solid-phase extraction, supercritical-fluid extraction, liquid-liquid extraction and QuEChERS are generally adopted in paddy sample preparation, and QuEChERS is the most frequently used method [26]. Analytical methods for pyrazosulfuron-ethyl and bensulfuron-methyl have been mainly focused on high performance liquid chromatography (HPLC) [27], capillary electrophoresis (CE) [28], gas chromatography-tandem mass spectrometry (GC-MS) [29], immunoassay [30], and liquid chromatography-tandem mass spectrometry (LC-MS) [31]. Acetochlor and butachlor analysis have been primarily performed on GC [32], HPLC [21], and GC-MS [33].…”

Section: Introductionmentioning

confidence: 99%

Dissipation Dynamics and Residue of Four Herbicides in Paddy Fields Using HPLC-MS/MS and GC-MS

Qian

Zhang

et al. 2019

IJERPH

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The dissipation dynamics and residue of pyrazosulfuron-ethyl, bensulfuron-methyl, acetochlor, and butachlor in paddy fields at Good Agricultural Practices (GAP) condition were carefully investigated in this study. The four herbicides’ residues were determined based on a quick, easy, cheap, rugged, safe (QuEChERS) method coupled with HPLC-MS/MS and GC-MS. The limit of detection (LOD) for pyrazosulfuron-ethyl, bensulfuron-methyl, acetochlor, and butachlor in all matrices ranged from 0.04–1.0 ng. The limit of quantification (LOQ) of the four herbicides ranged from 0.01–0.1 mg/kg. Moreover, the average recoveries of the four herbicides ranged from 78.9–108% with relative standard deviations (RSDs) less than 15% at three different fortified levels for different matrices. The dissipation results indicated that the average half-lives (t1/2) of the four herbicides in soil were in the range of 3.5–17.8 days, and more than 95% of the four herbicides dissipated within 5 days in water. Furthermore, the final residues of the four herbicides were all below the LOQ at harvest time. Such results highlight the dissipation dynamics and residue of the four herbicides in a rice cropping system and contribute to risk assessment as well as scientific guidance on the proper and safe application of herbicides in paddy fields.

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“…dc-ELISA. A dc-ELISA for residue analysis of emamectin benzoate was constructed as described previously (23). Rabbit anti-mouse IgG antibody (100 µL, 10 µg/mL) in PBS was added to each well of a 96-well microtiter plate, and the plate was incubated overnight at 4 °C.…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products

Kondo

Yamashita

Uchigashima

et al. 2008

J. Agric. Food Chem.

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A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3-3.0 ng/mL, and the 50% inhibition concentration (IC(50)) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 microg/g). Emamectin residues contain the following metabolites: the 4''-epi-amino analogue, the 4''-epi-(N-formyl)amino analogue, the 4''-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC(50) value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15-300 ng/g, and the recoveries were 91-117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.

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Simple Determination of Herbicides in Rice Paddy Water by Immunoassay

Cited by 6 publications

References 0 publications

Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl

Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl

Dissipation Dynamics and Residue of Four Herbicides in Paddy Fields Using HPLC-MS/MS and GC-MS

Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products

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