Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Abstract

This study reported simple surface modification for the immobilization of biomolecules such as proteins and cells onto desired area at micron-scale level. First, thin film composed of glycidyl methacrylate (GMA) was prepared by UV-photopolymerization. Then, the polyethylene glycol (PEG) microstructures which played a role in the prevention of nonspecific binding of biomolecules were fabricated by using micromolding in capillaries (MIMIC). Thus, we could easily obtain an orthogonal surface having biomolecular attraction and repulsion areas. In addition, we could control of the height of prepared PEG microstructures with spin coating or not. For the investigation of feasibility of biomolecule patterning onto the functionalized surface, FITC-BSA and HEK 293 were examined as representative biomolecule models. A functionalized surface with GMA promotes the strong adhesion of biomolecules, and PEG microstructures located on the background prevent nonspecific binding of biomolecules at micron-scale level. The orthogonal difference in surface functionality showed strong possibility of simple patterning of biomolecules. In addition, the proposed method could easily control the size, shape, and height of patterns. It will be useful platform technology for the construction of a biomolecule array.

Solvent Engineering Substantially Enhances the Chemoenzymatic Production of Surfactin

“…Cellular behavior is strongly influenced by the properties of the substrate 43–45. As indicated in literature PEG‐DMA structures are prepared on glass surfaces in most cases by whatever specialized surface functionalization like silanization or layer‐by‐layer deposition of charged polymers is required 33, 34, 37, 38, 42. Here, we demonstrate a preparation using cell culture dishes as substrate.…”

“…By curing PEG‐DMA against a polydimethylsiloxane (PDMS) mold, microwells for stem cell culture33, 34 or even structures on the nanolevel can be produced 35. Bottom‐free microstructures are achieved by applying the MIMIC (micromolding in capillaries) method 36–38. However, since PEG‐DMA layers do not stably attach to the commonly used glass surfaces, a preceding functionalization such as silanization is necessary.…”

Specificity of odorant-binding proteins: a factor influencing the sensitivity of olfactory receptor-based biosensors

“…Sensitivity is a highly important issue in the development of an effective biosensor [12][13][14]; thus, enormous efforts have been made to maximize sensitivity in various detection systems [15][16][17][18][19]. OBPs can be used to increase the sensitivity of olfactory receptor-based biosensors [20].…”