Simple HPLC method for simultaneous quantification of nicotine and cotinine levels in rat plasma after exposure to two different tobacco products

Abstract

Purpose Development and validation of a selective analytical method to accurately and precisely quantify nicotine and cotinine levels in rat's plasma after exposure to tobacco cigarettes and tobacco water-pipe. Methods An easy HPLC-Photodiode-Array Detection (PDA) method was developed and validated for simultaneous determination of nicotine and cotinine levels in plasma of 15 rats (10 rats after tobacco products exposure and 5 control rats). Nicotine and cotinine were extracted in one step from plasma using acetonitrile and concentrated to lowest volume using nitrogen stream. Results The developed method offered a rapid analysis time of 14 min with single step of analytes extraction from rat's plasma with recovery percentage range between 93 and 95% and excellent linearity with correlation factor more than 0.994 with analytical range between 50 and 1000 ng mL−1 and LOD of 25 ng mL−1 and 23 ng mL−1 for nicotine and cotinine, respectively. The analysis of rat's plasma after 28 days of exposure to tobacco cigarettes and tobacco water-pipe revealed that the average concentrations of 376 ng mL−1 for cotinine and 223 ng mL−1 for nicotine were obtained after tobacco cigarettes exposure, and 220 ng mL−1 for cotinine and 192 ng mL−1 for nicotine after tobacco water-pipe exposure. Conclusion Higher nicotine and cotinine levels were found in plasma after tobacco cigarettes exposure than water-pipe exposure which may have potential undesirable effects on passive smokers in both cases.

Acetylsalicylic acid reduces cigarette smoke withdrawal-induced anxiety in rats via modulating the expression of NFĸB, GLT-1, and xCT

“…Accordingly, if CO levels dropped under 500 ppm more cigarettes were used, and if CO levels rose above 899 ppm the pump was stopped for 1–2 min to bring CO levels back to the required range. Cotinine and nicotine levels were measured in our previous experiment using identical methods, the plasma concentration of nicotine was 222.98 ng/ml, and the plasma cotinine concentration was 376.12 ng/ml ( Alhusban et al, 2022 ).…”

Rapid and sensitive HPLC–MS/MS method for the quantification of dopamine, GABA, serotonin, glutamine and glutamate in rat brain regions after exposure to tobacco cigarettes

“…Whole‐body cigarette exposure for rats was performed using a well‐designed box made from acrylic with dimensions of 40 cm length, width and height, as described in our previous work (Alhusban, Hammad, & Alzaghari, 2022). Briefly, the exposure box had an upper door with three slots for ventilation and was connected to one pump.…”