Simple HPLC-UV method for the quantification of metformin in human plasma with one step protein precipitation

Chhetri, Himal Paudel; Thapa, Panna; Schepdael, Ann Van

doi:10.1016/j.jsps.2013.12.011

Cited by 81 publications

(40 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metformin, a biguanide-derivative antidiabetic drug, is the most widely used oral agent in humans (Brinkmann, Brixius, 2015;Chhetri, Thapa, Van Schepdael, 2014). It is the first-line drug for the treatment of type 2 diabetes, particularly, in overweight and obese patients with normal kidney function (American Diabetes, 2009;George, Joseph, 2014;Hung et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of metformin on semen quality

Banihani

2016

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

Various studies have linked metformin, a universally antidiabetic drug, with semen quality; however, such a direct link has not been established. This review systematically addresses and summarizes the effect of metformin on semen quality, particularly sperm function. We searched the MEDLINE electronic database for English articles and abstracts containing the key words 'metformin' and 'sperm', and relevant articles were reviewed. In summary, metformin appears to have improved and provided positive impact on sperm quality. This effect may be due to the ability of metformin to reduce oxidative stress and lipid peroxidation, enhance 5'-AMP activated protein kinase activity, and restore the normal levels of pituitary-gonadal hormones. However, further clinical research is still necessary to confirm such effect.Uniterms: Metformin/effects. Metformin/review. Metformin/semen quality. Sperm/study. Testosterone. Oxidative stress. 5'-AMP activated protein kinase. INTRODUCTIONMetformin, a biguanide-derivative antidiabetic drug, is the most widely used oral agent in humans (Brinkmann, Brixius, 2015;Chhetri, Thapa, Van Schepdael, 2014). It is the first-line drug for the treatment of type 2 diabetes, particularly, in overweight and obese patients with normal kidney function (American Diabetes, 2009;George, Joseph, 2014;Hung et al., 2015). In addition, recent research has proposed that metformin alone or in combination with insulin may be safe to treat gestational diabetes and does not increase the risk of metabolic acidosis (Huang, Castelino, Peterson, 2015; Kitwitee et al., 2015). Metformin's mechanism of action involves suppression of hepatic gluconeogenesis (Ferrannini, 2014; Foretz et al., 2010;Todd, Florez, 2014). One concern that has been raised relevant to metformin usage is that the consensus panel of diabetes felt that metformin should be considered for diabetes prevention even in non-diabetic individuals (i.e., pre-diabetic or obese individuals). This concern increases the use of this drug by these groups (American Diabetes, 2009;Hostalek, Gwilt, Hildemann, 2015;Manu et al., 2015;McGavock, Dart, Wicklow, 2015).Over the last decade, various experimental and clinical studies have linked metformin with sperm function, and thus with male factor infertility Ferreira et al., 2015); however, such direct link has not been established. In this review, we provide fresh and comprehensive understanding of the effect of metformin, as a universally used oral agent, on semen quality (i.e., sperm quantity and quality). To do this, we searched the MEDLINE electronic database for English-language articles and abstracts reported using the key words 'metformin' and 'sperm'. The references from selected articles were reviewed and used if relevant. Effect of metformin on sperm parameters Positive effectsTo date, the majority of studies linking metformin to sperm parameters are nonclinical. In his study on streptozotocin-induced diabetic rats, Attia, Helal, Alhaider (2009) made the seminal observation that metformin decreases the diabe...

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of metformin on semen quality

Banihani

2016

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…It improves glycemic control by decreasing hepatic glucose production, decreasing glucose absorption and increasing insulin-mediated glucose uptake [7] Various analytical methods have been reported for the estimation of Metformin Hydrochloride as alone as well as in combination with other drugs. They include Titrimetric method [8] , spectrophotometric methods [9][10][11] , HPLC [12][13][14] However an extensive literature search didn't reveal any estimation method for Empagliflozin and Metfromin hydrochloride drugs in their combined dosage form. Therefore, attempt was made to stability indicating method development and Validation of simple, precise, and accurate, RP-HPLC method for simultaneous determination of Empagliflozin and Metfromin hydrochloride drugs in their combined dosage form.…”

Section: Empagliflozin Is Chemically Designated As (2s3r4r5s6r)-mentioning

confidence: 99%

STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF EMPAGLIFLOZIN AND METFORMIN HCl

Patel¹

2017

WJPPS

View full text Add to dashboard Cite

One simple, specific, accurate, precise and reproducible and robust respectively. The % recoveries of Empagliflozin and Metformin HCl were found to be 99.60% -100.48% and 100.15% -100.23% respectively. Method was statistically validated for accuracy, precision, specificity, LOD, LOQ and robustness according to ICH guidelines.Developed method was found to be new, accurate, precise and rapid for simultaneous estimation of for Empagliflozin and Metformin HCl.

show abstract

“…In general, the use of a photodiode array (PDA) detector provides advantages for sensitive and selective HPLC systems during the analysis of drugs and their metabolites in biological matrices [15,16]. Using these principles, various analytical methods for determining plasma metformin levels have been widely developed and accepted [5,[17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Anticoagulants in Determining Metformin Hydrochloride Levels in Human Plasma Using High-Performance Liquid Chromatography

Harahap¹,

Marsudi

Sunarsih

2018

Int J App Pharm

View full text Add to dashboard Cite

Objective: This study aimed to develop and validate an analytical method and to determine the effect of different anticoagulants for analyzing metformin HCl level in human plasma. Methods:Analysis was performed with high-performance liquid chromatography method using a photodiode array detector set at 234 nm, a C 18 SunFire ™ column (5 µm, 250 mm×4.6 mm), a temperature of 40°C, a mobile phase comprising 10 mM sodium dodecyl sulfate and 10 mM phosphate buffer in water-acetonitrile (60:40 v/v) at pH 7.0, with a flow rate of 1.0 mL/min, and atorvastatin calcium as the internal standard. Plasma extraction was performed through protein precipitation using human plasma (300 µL) and acetonitrile (600 µL; 1:2 v/v). The method was linear at a concentration range of 20.0-5000.0 ng/mL, with r>0.9998. Results:The stability and recovery of metformin HCl in human plasma were not different between citrate with ethylenediaminetetraacetic acid (EDTA) and heparin with EDTA as anticoagulants. However, a significant difference in the peak area response ratio (p<0.05) was observed between the three anticoagulants. Conclusion:The validation results for the three types of plasma anticoagulants met validation requirements based on the European Medicines Agency bioanalytical guidelines of 2011 for accuracy and precision, selectivity, calibration curve linearity, dilution integrity, carry-over, and stability.

show abstract

Simple HPLC-UV method for the quantification of metformin in human plasma with one step protein precipitation

Cited by 81 publications

References 11 publications

Effect of metformin on semen quality

Effect of metformin on semen quality

STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF EMPAGLIFLOZIN AND METFORMIN HCl

Effect of Different Anticoagulants in Determining Metformin Hydrochloride Levels in Human Plasma Using High-Performance Liquid Chromatography

Contact Info

Product

Resources

About