Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

Ota, Noriyasu; Yalikun, Yaxiaer; Tanaka, Nobuyuki; Shen, Yingjia; Aishan, Yusufu; Nagahama, Yuki; Oikawa, Minoru; Tanaka, Yo

doi:10.2116/analsci.18p568

Cited by 8 publications

(11 citation statements)

References 37 publications

(39 reference statements)

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“…The liquid pre‐polymer base and the cross‐linking agent were mixed at a ratio of 10:1 by weight. Then, mixed PDMS was poured over the master to create a layer of 2–10 mm thickness and the coated master was heated at 80°C in an oven for 1 h. The cured PDMS was then carefully peeled off the master and trimmed to the suitable size . This PDMS layer and a glass slide (70 × 30 mm, 0.7‐mm thickness) were then exposed to oxygen plasma (10 W, 10 s) in a plasma generator (FA‐1; SAMCO, Tokyo, Japan) to form the PDMS microfluidic device by bonding the plasma‐treated layers.…”

Section: Methodsmentioning

confidence: 99%

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

et al. 2019

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Imaging flow cytometry is a powerful tool by virtue of its capability for high‐throughput cell analysis. The advent of high‐speed optical imaging methods on a microfluidic platform has significantly improved cell throughput and brought many degrees of freedom to instrumentation and applications over the last decade, but it also poses a predicament on microfluidic chips. Specifically, as the throughput increases, the flow speed also increases (currently reaching 10 m/s): consequently, the increased hydrodynamic pressure on the microfluidic chip deforms the wall of the microchannel and produces detrimental effects lead to defocused and blur image. Here, we present a comprehensive study of the effects of flow‐induced microfluidic chip wall deformation on imaging flow cytometry. We fabricated three types of microfluidic chips with the same geometry and different degrees of stiffness made of polydimethylsiloxane (PDMS) and glass to investigate material influence on image quality. First, we found the maximum deformation of a PDMS microchannel was >60 μm at a pressure of 0.6 MPa, while no appreciable deformation was identified in a glass microchannel at the same pressure. Second, we found the deviation of lag time that indicating velocity difference of migrating microbeads due to the deformation of the microchannel was 29.3 ms in a PDMS microchannel and 14.9 ms in a glass microchannel. Third, the glass microchannel focused cells into a slightly narrower stream in the X‐Y plane and a significantly narrower stream in the Z‐axis direction (focusing percentages were increased 30%, 32%, and 5.7% in the glass channel at flow velocities of 0.5, 1.5, and 3 m/s, respectively), and the glass microchannel showed stabler equilibrium positions of focused cells regardless of flow velocity. Finally, we achieved the world's fastest imaging flow cytometry by combining a glass microfluidic device with an optofluidic time‐stretch microscopy imaging technique at a flow velocity of 25 m/s. © 2019 International Society for Advancement of Cytometry

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Section: Methodsmentioning

confidence: 99%

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

et al. 2019

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“…In general, the materials for the fabrication of a microfluidic device should meet the following general criteria: 1) the device materials should have favorable chemical and biological compatibility with the working medium; 2) the surface of the device material should be easily modified; 3) the cost of device materials should be low . These microfluidic chip materials mainly include inorganic materials, such as silicon wafers, quartz, glass, or organic polymer materials, including polymethyl methacrylate (PMMA), polydimethylsiloxane (PDMS), and polycarbonate (PC). Among inorganic materials have been widely used to fabricate microfluidic devices with desirable patterns and channel shapes because of their good chemical inertia, thermal stability and ease of machinability.…”

Section: The Review Scope Of Microfluidic Devicesmentioning

confidence: 99%

Recent Progress of Microfluidic Devices for Hemodialysis

Luo

Fan

Wang

2019

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Microfluidic hemodialysis techniques have recently attracted great attention in the treatment of kidney disease due to their advantages of portability and wearability as well as their great potential for replacing clinical hospital‐centered blood purification with continuous in‐home hemodialysis. This Review summarizes the recent progress in microfluidic devices for hemodialysis. First, the history of kidney‐inspired hemodialysis is introduced. Then, recent achievements in the preparation of microfluidic devices and hemodialysis nanoporous membrane materials are presented and categorized. Subsequently, attention is drawn to the recent progress of nanoporous membrane‐based microfluidic devices for hemodialysis. Finally, the challenges and opportunities of hemodialysis microfluidic devices in the future are also discussed. This Review is expected to provide a comprehensive guide for the design of hemodialysis microfluidic devices that are closely related to clinical applications.

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“…Moreover, cells have recently been used in drug discovery, drug development, biomedical research, and biological technology and medical applications. Recently, various types of cell separation methods have been developed 5,6 . Especially, a cell separation method with maintaining cell activity is strongly required for cell transplantation.…”

Section: Introductionmentioning

confidence: 99%

Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

et al. 2021

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In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperatureresponsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.

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Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

Cited by 8 publications

References 37 publications

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

Recent Progress of Microfluidic Devices for Hemodialysis

Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

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