Simple Method for Measuring the Peroxide Value in a Colored Lipid

Abstract

We developed a simple method for quantification of the peroxide value (PV) in colored lipids on the basis of the reaction between triphenylphosphine (TPP) and oxidized oil to afford triphenylphosphineoxide (TPPO). Diphenylphosphineoxide (DPPO) was employed as internal standard. The formed TPPO was analyzed by high-pressure liquid chromatography-UV spectroscopy with absorption at 260 nm. The conditions that gave the highest correlative calibration curve between the peak area on the chromatogram and peroxide value were identified: the optimum TPP-oxidized oil mix ratio, reaction temperature, and reaction time were found to be 2:1, 40°C, and 30 min, respectively. Linear calibration curves, passing through the origin, were obtained for PV versus TPPO and TPPO versus DPPO. The quantification limit for this method was 2.01 pmol hydroperoxyl group, which corresponds to a PV value of 0.2 meq/kg in a 10-mg oil sample. This method was used to measure the PV in colored fats and oils or lipids extracted from dark meat and processed food containing a coloring agent. Though the official method could not measure the PVs in the colored lipids, the method proposed here, which uses an inexpensive chemical reagent and machine, could. The developed method could play an important role for food quality control.

Survey of Oxidative Characteristics of Used Frying Oils in Falafel Shops in Arak City

“…1 , Tajik, R. 2 , Navabi, A. 3 , MoradZadeh, R. 4 , Abdollahi, A. 5 , Asafari, M. 6 * Total oxidation value, Frying Oil.…”

Investigating the Changes in Physicochemical and Oxidative Properties of Different Brands of Sunflower Oil Sold in Market Before and After Frying

“…These can compromise the texture, taste, flavor, and the overall perception of the fried product. Additionally, potential risk to human health and nutrition may arise [11].…”

Measuring Primary Lipid Oxidation in Food Products Enriched with Colored Microalgae

“…The addition of triphenylphosphine (TPP) to the FOX method can help to eliminate the interference. TPP forms a complex with the hydroperoxides present in the sample to form TPPO (Gotoh et al, 2011;Wang et al, 2016). By adding an excess of TPP, none of the hydroperoxides can oxidize Fe 2+ to Fe 3+ , and thus lead to an increase in the complex formation of xylenol.…”