Simple Method of Paroxetine Determination Usinga Single Channe lFIA with No In-line Reaction Process

Altıokka, Göksel; Kircali, Kevser

doi:10.2116/analsci.19.629

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…The results were in accordance with those given by a reference method 15) as revealed by statistical analysis adopting Student's t-test and F-test where no significant difference was noticed between the two methods; ( Table 1). The validity of the procedure was further assured by the recovery of standard addition, ( Table 2).…”

supporting

confidence: 87%

“…Other reported methods are one voltammetric 14) and one FIA. 15) Reviewing the literature revealed that, up to the present time, nothing has been reported concerning the spectrofluorimetric determination of paroxetine. This encourages the use of the native fluorescence of paroxetine to develop a simple and sensitive fluorimetric method for its determination in formulations and human plasma.…”

Section: -3)mentioning

confidence: 95%

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Spectrofluorimetric Determination of Paroxetine Hydrochloride in Its Formulations and Human Plasma

Alarfaj¹,

Razeq²,

Sultan

2006

Chem. Pharm. Bull.

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Paroxetine-HCl is a new selective serotonin reuptake inhibitor used as antidepressant. [1][2][3] Most of the reported methods for the analysis of paroxetine in biological fluids and breast milk rely on the use of chromatographic techniques including TLC, 4,5) HPLC [6][7][8][9] and GC 10,11) in addition to electrophores. 12,13) Although chromatographic methods are highly selective however, their requirements of cleaning up samples and sophisticated instrumentation preclude their use in routine analysis. Other reported methods are one voltammetric 14) and one FIA. 15)Reviewing the literature revealed that, up to the present time, nothing has been reported concerning the spectrofluorimetric determination of paroxetine. This encourages the use of the native fluorescence of paroxetine to develop a simple and sensitive fluorimetric method for its determination in formulations and human plasma. ExperimentalApparatus Fluorimetric measurements were performed using a spectrofluorimeter (Jasco model FP6200, Japan) equipped with Xenon discharge lamp and 1 cm quartz cell at medium sensitivity.Reagents and Materials A reference standard of paroxetine-HCl was kindly supplied by Saudi Pharmaceutical Industries and Medical Appliances corporation (Buraydah, Saudi Arabia). Zeroxat C.R. tablets each labeled to contain 20 mg paroxetine as paroxetine HCl (manufactured by Smith Kline Beecham Pharmaceuticals, England) were purchased from local market. Plasma was obtained from king Khalid University Hospital, Riyadh KSA and kept frozen until used after gentle thawing.A stock solution of paroxetine-HCl (0.5 mg ml Ϫ1) in methanol (Merck, Germany) was prepared, which was found to be stable for up to 30 d if kept in a refrigerator protected from light. Working standard solution (5 mg ml Ϫ1 ) was prepared by dilution with methanol as appropriate.Procedure. Calibration Curve Accurate volumes (0.25-2 ml) of the working standard solution of paroxetine-HCl (5 mg ml Ϫ1 in methanol) were transferred into a series of 25 ml volumetric flasks and completed to the mark with methanol. The fluorescence intensity was measured at 340 nm after excitation at 290 nm. To get the calibration curve, the fluorescence intensity was plotted versus the final concentration of the drug and the corresponding regression equation was derived.Analysis of Tablets Ten zeroxat CR tablets were weighed and pulverized. An accurate weight of the fine powder equivalent to 25 mg of paroxetine-HCl were transferred to a small beaker and extracted with 40 ml methanol by sonnication for 20 min, then filtered into a 50-ml volumetric flask. The flasks were completed to volume after washing the residue with methanol.The above procedure under "Calibration curve" was then followed and the nominal contents of tablets were calculated using the regression equation.Analysis of Spiked Human Plasma Different aliquots (0.2-0.8) ml of the working drug solution 5 mg ml Ϫ1 in methanol were added to 1.0 ml plasma in five standard centrifuge tubes and the volume was completed to 10 ml with methanol. ...

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supporting

confidence: 87%

Section: -3)mentioning

confidence: 95%

Spectrofluorimetric Determination of Paroxetine Hydrochloride in Its Formulations and Human Plasma

Alarfaj¹,

Razeq²,

Sultan

2006

Chem. Pharm. Bull.

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“…During the past 6 years the majority of these methods were based on chromatography; high-performance liquid chromatography coupled with ultraviolet/diode array [1][2][3][4][5][6][7][8][9][10][11], mass [12][13][14][15][16][17][18], fluorimetric [19,20], and coulometric [21] detectors; gas chromatography with mass [22][23][24], flame-ionisation [25], nitrogen-phosphorus [26] and electron-capture [27] detection; micellar electrokinetic capillary chromatography [28]; and thin layer chromatography [9,29]. Capillary electrophoresis [30][31][32], flowinjection analysis with ultraviolet detection [33], and visible spectrophotometry [34,35] have also been applied in the analysis of PRX.…”

Section: Introductionmentioning

confidence: 99%

Electroanalytical determination of paroxetine in pharmaceuticals

Nouws

Delerue–Matos

Barros

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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Electroanalytical methods based on square-wave adsorptive-stripping voltammetry (SWAdSV) and flow-injection analysis with SWAdSV detection (FIA-SWAdSV) were developed for the determination of paroxetine (PRX). The methods were based on the reduction of PRX at a mercury drop electrode at −1.55 V versus Ag/AgCl, in a borate buffer of pH 8.8, and the possibility of accumulating the compound at the electrode surface. Because the presence of dissolved oxygen did not interfere significantly with the analysis, it was also possible to determine PRX using FIASWAdSV. This method enables analysis of up to 120 samples per hour at reduced costs. Both methods developed were validated and successfully applied to the quantification of PRX in pharmaceutical products.

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Review of recent applications of flow injection spectrophotometry to pharmaceutical analysis

Tzanavaras

Themelis

2007

Analytica Chimica Acta

102

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Simple Method of Paroxetine Determination Usinga Single Channe lFIA with No In-line Reaction Process

Cited by 7 publications

References 19 publications

Spectrofluorimetric Determination of Paroxetine Hydrochloride in Its Formulations and Human Plasma

Spectrofluorimetric Determination of Paroxetine Hydrochloride in Its Formulations and Human Plasma

Electroanalytical determination of paroxetine in pharmaceuticals

Review of recent applications of flow injection spectrophotometry to pharmaceutical analysis

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