Simple Nanoparticle-Based Luminometric Method for Molecular Weight Determination of Polymeric Compounds

Pihlasalo, Sari; Virtamo, Maria; Legrand, Nicolas; Hänninen, Pekka; Härmä, Harri

doi:10.1021/ac401797m

Cited by 3 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymeric and silica nanoparticles doped with lanthanide complexes are emerging and prospective nanomaterials for sensing purposes [13,[33][34][35]. These data along with our previous report [15] on polyelectrolyte-deposited colloids indicate that the ternary complex formation of the analytes with lanthanide-based colloids can induce their luminescent response.…”

Section: Tb(iii)-centered Response Of the Colloids On The Chelating Ssupporting

confidence: 59%

Interfacial interactions of hard polyelectrolyte-stabilized luminescent colloids with substrates

Shamsutdinova

Zairov

Mustafina

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

Section: Tb(iii)-centered Response Of the Colloids On The Chelating Ssupporting

confidence: 59%

Interfacial interactions of hard polyelectrolyte-stabilized luminescent colloids with substrates

Shamsutdinova

Zairov

Mustafina

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

“…29,30 Recently, we demonstrated the novel application of the nanoparticlebased method utilizing TR-LRET for the determination of MW for polyethylenimines and polyamino acids. 31 The method utilized the size-dependent competitive adsorption between differently sized polymers and labeled protein to nanoparticles. These molecules have a total positive charge at the assay pH and polyamino acids contain hydrophobic sites giving optimal attractive electrostatic and hydrophobic interactions for adsorption to carboxylate-modified polystyrene particles.…”

mentioning

confidence: 99%

“…The assessment of PEG MW was enabled without prior knowledge of the PEG concentration, which was not achieved in our previous work. 31 ■ EXPERIMENTAL SECTION Materials. γ-Globulins from bovine blood (γG), albumin from bovine serum (BSA), and polyethylene glycols (PEG 200, PEG 400, PEG 1500, PEG 3000, PEG 6000, and PEG 35000) were from Sigma-Aldrich Co. (St. Louis, MO).…”

mentioning

confidence: 99%

“…Previously, we have developed the nanoparticle-based methods utilizing time-resolved luminescence resonance energy transfer (TR-LRET) and quenching for the quantification of proteins, − eukaryotic cells, and detergents. , Recently, we demonstrated the novel application of the nanoparticle-based method utilizing TR-LRET for the determination of MW for polyethylenimines and polyamino acids . The method utilized the size-dependent competitive adsorption between differently sized polymers and labeled protein to nanoparticles.…”

mentioning

confidence: 99%

“…The method was tested for PEG polymers with MW from 62 (monomer) to 35 000 g/mol. The assessment of PEG MW was enabled without prior knowledge of the PEG concentration, which was not achieved in our previous work …”

mentioning

confidence: 99%

See 2 more Smart Citations

Method for Determination of Polyethylene Glycol Molecular Weight

2015

Self Cite

View full text Add to dashboard Cite

A method utilizing competitive adsorption between polyethylene glycols (PEGs) and labeled protein to nanoparticles was developed for the determination of PEG molecular weight (MW) in a microtiter plate format. Two mix-and-measure systems, time-resolved luminescence resonance energy transfer (TR-LRET) with donor europium(III) polystyrene nanoparticles and acceptor-labeled protein and quenching with quencher gold nanoparticles and fluorescently labeled protein were compared for their performance. MW is estimated from the PEG MW dependent changes in the competitive adsorption properties, which are presented as the luminescence signal vs PEG mass concentration. The curves obtained with the TR-LRET system overlapped for PEGs larger than 400 g/mol providing no information on MW. Distinctly different curves were obtained with the quenching system enabling the assessment of PEG MW within a broad dynamic range. The data was processed with and without prior knowledge of the PEG concentration to measure PEGs over a MW range from 62 to 35,000 g/mol. The demonstration of the measurement independent of the PEG concentration suggests that the estimation of MW is possible with quenching nanoparticle system for neutrally charged and relatively hydrophilic polymeric molecules widening the applicability of the simple and cost-effective nanoparticle-based methods.

show abstract

Nonspecific luminometric assay for monitoring protein adsorption efficiency and coverage on nanoparticles

2017

View full text Add to dashboard Cite

Nonspecific assays utilizing time-resolved luminescence resonance energy transfer (TR-LRET) are developed for two applications: to monitor protein adsorption efficiency and to assess the degree of surface coverage on the solid phase. We successfully measure the adsorption efficiency of non-sedimenting nanoparticles since this has been notoriously difficult to determine. Monitoring of the protein adsorption on nanoparticles does not require the nanoparticles with the adsorbed protein to be washed and it is based on the competitive adsorption between the non-adsorbed analyte protein and the acceptor-labeled protein to donor europium(iii) polystyrene nanoparticles. The application for assessing the degree of surface coverage is performed with the final coated and washed analyte nanoparticles and it requires no fluorescent labeling of the studied protein. This application utilizes the competitive adsorption of the acceptor-labeled protein on analyte nanoparticles partly covered with protein and donor europium(iii) polystyrene nanoparticles. The developed methods detect either non-adsorbed protein or uncovered nanoparticle surface, not the proteins adsorbed on the nanoparticles. This is not achievable with the traditional total protein quantification assays applied for monitoring protein adsorption since both non-adsorbed and adsorbed protein are detected and their separation is required. Thus, the developed application for monitoring protein adsorption is user-friendly, requires no centrifugal instrumentation, and is applicable also for small nanoparticles requiring ultracentrifugation. No special expertise or investment in costly instruments is required compared to the existing methods, such as spectroscopic techniques, isothermal titration calorimetry, and surface plasmon resonance. The application for assessing the degree of surface coverage is compared to a reference literature method that comprised the analysis of adsorbed fluorescently labeled protein. The saturation reached at similar protein concentrations showing the reliability of the assay. Our results suggest that the developed applications could be exploited as rapid tools for protein adsorption studies on nanoparticles and for quality control and characterization of the coating processes.

show abstract

Simple Nanoparticle-Based Luminometric Method for Molecular Weight Determination of Polymeric Compounds

Cited by 3 publications

References 30 publications

Interfacial interactions of hard polyelectrolyte-stabilized luminescent colloids with substrates

Interfacial interactions of hard polyelectrolyte-stabilized luminescent colloids with substrates

Method for Determination of Polyethylene Glycol Molecular Weight

Nonspecific luminometric assay for monitoring protein adsorption efficiency and coverage on nanoparticles

Contact Info

Product

Resources

About