2016
DOI: 10.1063/1.4941776
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Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Abstract: The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differe… Show more

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Cited by 14 publications
(17 citation statements)
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“…R1_ MHC-neor/pGK-hygro ES cells (clone HG8) carrying the Myh6 promotor regulating the expression of neomycin phosphotransferase were prepared by the transfection of R1 cells by MHC-neor/pGK-hygro plasmid (kindly donated by dr. Loren J. Field, Krannert Institute of Cardiology, Indianapolis, US [29])[30]. Both R1 subclones undergo cardiomyogenesis in the same manner as the maternal cell line, which was verified in our preliminary experiments (not shown).…”
Section: Methodssupporting
confidence: 59%
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“…R1_ MHC-neor/pGK-hygro ES cells (clone HG8) carrying the Myh6 promotor regulating the expression of neomycin phosphotransferase were prepared by the transfection of R1 cells by MHC-neor/pGK-hygro plasmid (kindly donated by dr. Loren J. Field, Krannert Institute of Cardiology, Indianapolis, US [29])[30]. Both R1 subclones undergo cardiomyogenesis in the same manner as the maternal cell line, which was verified in our preliminary experiments (not shown).…”
Section: Methodssupporting
confidence: 59%
“…Cardiomyocytes form compact clusters similar to the functional syncitium of myocard; quantitative isolation is thus complicated by the potential loss of cells or whole clusters. To verify our results, the indirect quantification of cardiomyocytes selected on the basis of their gene expression was performed[30]. The increased level of cardiomyocyte markers in mixed differentiation populations is extrapolated from the number of other generated cell types within prolonged EBs differentiation.…”
Section: Discussionmentioning
confidence: 91%
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