Simple, one-step dye-based kit for bacterial contamination detection in a range of water sources

Kumar, Sweta Binod; Shinde, Ambika Hemant; Mehta, Romil; Bhattacharya, Amit; Haldar, Soumya

doi:10.1016/j.snb.2018.08.077

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…Regular detection of bacteria in various water sources such as municipal water, water supply for businesses or homes, recreational waters, and so on is needed to identify and prevent potential health/environmental risks to ensure public health globally [14].…”

Section: Relationship Between the Use Of Spring Water And The Inciden...mentioning

confidence: 99%

Relationship between water sources and diarrhea incidence in North Buton Regency

Jumakil,

Asnia Zainuddin

2024

World J. Adv. Res. Rev.

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Background: The condition of water sources in the unprotected category is classified as unsafe sanitation facilities. This is very risky for endangering the people who use it as a source of clean water for their daily needs. In North Buton Regency, based on monitoring, unprotected water sources are still found, both dug wells and springs which are used by the community to meet their clean water needs. Method: quantitative analytical, with a cross sectional study design. This research was carried out using a household survey method using a questionnaire, as well as observationally using an observation sheet. The sample in this study was 3,640 households, this number was obtained from 91 villages, where each village was represented by 40 households selected using the simple random sampling method. Result: The results of this study show that there is a significant relationship between the use of dug well water sources and the incidence of diarrhea based on statistical tests using the chi square test, obtaining a p value of 0.000 < 0.05. There is a relationship between the use of spring water and the incidence of diarrhea in North Buton Regency with a p value of 0.000 < 0.05. Conclusion: There is a significant relationship between dug wells and spring water sources and the incidence of diarrhea in North Buton Regency.

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Section: Relationship Between the Use Of Spring Water And The Inciden...mentioning

confidence: 99%

Relationship between water sources and diarrhea incidence in North Buton Regency

Jumakil,

Asnia Zainuddin

2024

World J. Adv. Res. Rev.

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“…The U-AST kit for the detection of urinary tract infection along with the antibiotic-sensitivity test was prepared as described in our previous work. 27,28 For the 7% PVDF membrane, PVDF (polyvinylidene uoride), DMF (dimethylformamide), graphene oxide, tetrazolium dye, nutrient broth, nutrient agar, MacConkey agar, and glucose were purchased from HiMedia, India. All the commercial antibiotics, namely amikacin (1st class aminoglycoside), amoxicillin (penicillin), ampicillin (aminopenicillin), ciprooxacin (uoroquinolone), and co-trimoxazole (sulfonamide) were purchased from HiMedia, India.…”

Section: Materials Usedmentioning

confidence: 99%

Market-ready U-AST kit: simple, fast, cost-effective solution for concurrently detecting urinary tract infection and antibiotic resistance

Behere,

Haldar

2024

Anal. Methods

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“…Most of the existing portable fluorometers [30][31][32][33][34] have a very higher limit of detection (7 to 10 5 CFU/mL) requiring complicated procedures i.e., preparation of magnetic nanoparticles and detection time is usually higher (around 30 minutes). On the other hand, our multichannel fluorometer has a LOD of <1 CFU/mL with incubation and filtration and detection can be done within as low as 20 seconds.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the existing portable fluorometers − have a very higher LOD (7–10 5 cfu/mL) requiring complicated procedures, that is, preparation of magnetic nanoparticles, and detection time is usually higher (around 30 min). On the other hand, our multichannel fluorometer has an LOD of <1 cfu/mL with incubation and filtration and detection being done within as low as 20 s. This multichannel device has the ability for parallel testing of samples, which presents a significant improvement over our earlier prototype. , It can feature an inherent negative control and has the potential to detect anaerobes.…”

Section: Introductionmentioning

confidence: 99%

Rapid Ultrasensitive and High-Throughput Bioburden Detection: Microfluidics and Instrumentation

et al. 2022

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Contamination detection often requires lengthy culturing steps to detect low-level bioburden. To increase the rate of detection and decrease the limit of detection (LOD), a system featuring microfluidics and a multichannel fluorometer has been developed. The 8-channel fluorometer enables parallel testing of multiple samples with LOD as low as < 1 CFU/mL. This low-cost system utilizes the slope of fluorescence intensity that serves as the criterion for bioburden detection. The redox indicator dye resazurin is used to monitor the presence of viable cells, in this study and is reduced to resorufin with a high quantum yield at 585 nm. The sample under investigation is spiked with resazurin, loaded in a special-design microfluidic cassette and the rate of change is observed via the fluorometer. The method was validated using primary E. coli culture in comparison with a spectrophotometer which served as the gold standard. An optimized assay based on LB media was developed. The impact on the assay sensitivity based on incubation and filtration steps was also explored. The assay is shown to pick up inadvertent contamination from test tubes and pipette tips showing its applicability in real-world settings. The data analysis demonstrated a comparable performance of the multichannel fluorometer vis-a-vis the conventional plate reader. The multichannel system is shown to detect bioburden presence in as low as 20 seconds for bacterial concentrations ≥ 5 CFU/mL after 6 hours of incubation. Considering its portability, low cost, simplicity of operation and relevant assay sensitivity, the system is well-positioned to detect low-level bioburden in the laboratory, pharmaceutical and field settings.

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Simple, one-step dye-based kit for bacterial contamination detection in a range of water sources

Cited by 10 publications

References 22 publications

Relationship between water sources and diarrhea incidence in North Buton Regency

Relationship between water sources and diarrhea incidence in North Buton Regency

Market-ready U-AST kit: simple, fast, cost-effective solution for concurrently detecting urinary tract infection and antibiotic resistance

Rapid Ultrasensitive and High-Throughput Bioburden Detection: Microfluidics and Instrumentation

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