Simple preparative two-step purification of interleukin-2 from culture medium of lectin stimulated normal human lymphocytes

Interleukin-2: Evaluation of Routes of Administration and Current Delivery Systems in Cancer Therapy

“…Natural human IL‐2 (hIL‐2) is a hydrophobic 15.5 kDa globular glycosylated protein with a various degree of glycosylation, as estimated from gel filtration. It is glycosylated at position 3 by the amino acid thrieonine 47. Its primary structure is represented by 133 amino acids, which shows no significant homologies in the primary structure with other cytokines 48, 49.…”

Correlation between the Phenotype and the Functional Capacity of Activated T Cells in Patients with Active Systemic Lupus Erythematosus

“…The IL-2-dependent proliferation was determined by incubation of MNC in the presence of 10 U/ml leetin-free IL-2 with or without addition of 1 ;Mg/ml eoncanavalin A (Con A) (Sifin) for 72 h. One microcurie of [-'H]thymidine was added to each well 12-18 h before harvesting. Lectin-free IL-2 was prepared as described in detail elsewhere [5]. In brief, MNC were stimulated with Con A and phorbol myristate acetate (PMA) in serum-free Iscove's medium for 6 h. After washing, the cells were cultured for a further 2 days without Con A.…”

“…The cell-free supernatant was tested for IL-2 activity on a mouse CTL line. One unit of IL-2 per ml was defined as the IL-2 concentration which gives half the maximum proliferation in the standard assay [5].…”