Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography

Rojas, Alfonso Mazuelos; Sandron, Sara; Wilson, Richard; Davies, Noel W.; Haddad, Paul R.; Shellie, Robert A.; Nesterenko, Pavel N.; Paull, Brett

doi:10.1016/j.aca.2016.01.003

Cited by 7 publications

(10 citation statements)

References 30 publications

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“…5,12−14 In the absence of a reference standard, as in the analysis of natural complex mixtures, quantitative information can be achieved only by employing a universal detector. 14 A technique able to unambiguously detect all nonvolatile material in a sample and achieve a near uniform response is charged aerosol detection (CAD). The detector response is independent from the physiochemical properties of the material and its signal is proportional only to the amount of material transferred to the analyzer.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Among the techniques sensitive enough to detect low concentrations, none are able to detect all the species constituting DOM; therefore, a combination of multiple techniques is advisable in order to gain more insights into the composition of the natural material. The combination of qualitative and quantitative techniques is promising because it allows contextualization of physicochemical properties of the material according to its abundance. ,− In the absence of a reference standard, as in the analysis of natural complex mixtures, quantitative information can be achieved only by employing a universal detector . A technique able to unambiguously detect all nonvolatile material in a sample and achieve a near uniform response is charged aerosol detection (CAD).…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Ionization of Dissolved Organic Matter by Electrospray

et al. 2020

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Electrospray ionization (ESI) operating in the negative mode coupled to high-resolution mass spectrometry is the most popular technique for the characterization of dissolved organic matter (DOM). The vast molecular heterogeneity and the functional group diversity of this complex mixture prevents the efficient ionization of the organic material by a single ionization source, so the presence of uncharacterized material is unavoidable. The extent of this poorly ionizable pool of carbon is unknown, is presumably variable between samples, and can only be assessed by the combination of analysis with a uniform detection method. Charged aerosol detection (CAD), whose response is proportional to the amount of nonvolatile material and is independent from the physicochemical properties of the analytes, is a suitable candidate. In this study, a fulvic acid mixture was fractionated and analyzed by high-pressure liquid chromatography–mass spectrometry in order to investigate the polarity and size distributions of highly and poorly ionizable material in the sample. Additionally, DOM samples of terrestrial and marine origins were analyzed to evaluate the variability of these pools across the land–sea aquatic continuum. The relative response factor values indicated that highly ionizable components of aquatic DOM mixtures are more hydrophilic and have lower molecular weight than poorly ionizable components. Additionally, a discrepancy between the samples of terrestrial and marine origins was found, indicating that marine samples are better represented by ESI than terrestrial samples, which have an abundant portion of hydrophobic poorly ionizable material.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating the Ionization of Dissolved Organic Matter by Electrospray

et al. 2020

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“…This technique has a high capacity as well as excellent sample recovery and a wider selection range of solvent systems. Furthermore, it permits the introduction of a crude sample directly into the hollow column .…”

Section: Introductionmentioning

confidence: 99%

Bioactivity screening, extraction, and separation of lactate dehydrogenase inhibitors fromPolygala tenuifoliaWilld. based on a hyphenated strategy

Liu

et al. 2017

J. Sep. Sci.

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Stroke is the second leading cause of death worldwide. Lactate dehydrogenase inhibitors are currently widely used in the treatment of ischemic stroke, and natural products are considered promising sources of lactate dehydrogenase inhibitors. In this study, ultrafiltration liquid chromatography coupled with mass spectrometry was used for the screening and identification of lactate dehydrogenase inhibitors from Polygala tenuifolia. Furthermore, five lactate dehydrogenase inhibitors, sibiricose A5, 3,6'-di-O-sinapoyl-sucrose, glomeratose A, tenuifoliside B, and tenuifoliside C, were selected as target lactate dehydrogenase inhibitors. In addition, the five target compounds with purities of 96.45, 97.65, 96.38, 94.34, and 93.29% were extracted and isolated using a new hyphenated strategy of microwave-assisted extraction coupled with countercurrent chromatography with a two-phase solvent system of n-hexane/n-butanol/ethanol/water (5.321:1.00:1.664:6.647). The bioactivities of the isolated compounds were analyzed using PC12 cells and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The results also demonstrated that microwave-assisted extraction coupled with countercurrent chromatography is an efficient method of isolating chemical constituents from medicinal herbs. Moreover, the research route consisting of activity screening, extraction, separation, and activity verification of the compounds has the advantages of being efficient, orientated, and objective.

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“…Antiplasmodial activity related to alkaloids has been confirmed; flavonols were also isolated from the extract and purified. Rojas et al [7] have quantified the dissolved organic matter in waters after solid-phase extraction by a CCC method with a sequentially connected UV detector and an ELSD.…”

Section: Introductionmentioning

confidence: 99%

“…ELSDs are known to exhibit a non-linear response, although some authors have reported linear calibration curves [7,12]. The calibration curve can be linearized by using single-point calibration [13], which has been adapted and applied successfully to the mutarotation monitoring of monosaccharides [14].…”

Section: Introductionmentioning

confidence: 99%

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

Pazourek

Šmejkal

2016

Molecules

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Abstract:The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-D-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.

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Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography

Cited by 7 publications

References 30 publications

Investigating the Ionization of Dissolved Organic Matter by Electrospray

Investigating the Ionization of Dissolved Organic Matter by Electrospray

Bioactivity screening, extraction, and separation of lactate dehydrogenase inhibitors fromPolygala tenuifoliaWilld. based on a hyphenated strategy

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

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