Simple, Rapid and Selective Chronopotentiometric Method for the Determination of Riboflavin in Pharmaceutical Preparations Using a Glassy Carbon Electrode

Abstract

A novel, simple, sensitive and reliable electrochemical method for the riboflavin determination using chronopotentiometry with glassy carbon electrode was developed. The most important instrumental parameters of chronopotentiometry including type and concentration of supporting electrolyte, initial potential and current range were examined and optimised in respect to riboflavin analytical signal. Riboflavin provided well defined reduction signal at -0.12 V vs. Ag/AgCl (3.5 mol/L KCl) electrode in 0.025 mol/L HCl. Under optimal conditions, linear response of riboflavin was observed in the concentration range of 0.2-70 mg/L with achieved limit of detection of 0.076 mg/L and limit of quantitation of 0.23 mg/L of riboflavin. Common vitamins and excipients did not interfere with the determination. The proposed method was successfully applied for determination of riboflavin in commercially available pharmaceutical preparations. The obtained results were in statistical agreement to the contents declared by manufacturer and to those obtained by HPLC used as a comparative method.

Synthesis of highly fluorescent carbon dots from lemon and onion juices for determination of riboflavin in multivitamin/mineral supplements

“…The selectivity of the proposed method using CDs for the determination of riboflavin in multivitamin/mineral supplements was also investigated. For this purpose, potential interfering substances [18], including folic, citric, L-ascorbic and pantothenic acid, biotin, cyanocobalamin, niacin, thiamin, sucrose, d -glucose, fructose, Cu 2+ , Zn 2+ , Fe 2+ , Fe 3+ , Mg 2+ , Ca 2+ , Na + , and K + in a ratio interferents/riboflavin 100:1.0 μg/mL were analyzed. A compound was considered “non-interfering” if the change of fluorescence intensity was ±5% as compared to that obtained in its absence [18].…”

“…Nine commercially-available samples from three different brands of multivitamin/mineral supplements were purchased from local retail suppliers in João Pessoa, Paraíba, Brazil. These samples were prepared for analysis according to the following procedure described elsewhere [18,23]: five tablets of each sample were weighed and powdered in a grinding mortar, and were dissolved in 50 mL of deionized water and sonicated for 15 min. After, the sample solution was filtered to remove undissolved residue, and diluted with deionized water to the required concentration of riboflavin.…”

“…Riboflavin, commonly known as vitamin B 2 , is one of the water-soluble vitamins that play a crucial role in functioning healthy humans. It is also required for a wide variety of cellular processes, such as metabolism of fats, ketone bodies, carbohydrates, proteins, nucleic acid repair process, cell apoptosis and electron transfer processes in the respiratory chain [14,18]. Although riboflavin is found in many foods, such as milk and dairy products, meat, eggs, livers, cereals and fresh leafy vegetables [18], diets lacking this vitamin cause lesions of the muco-cutaneous surfaces, intense photophobia, fatigue, slowed growth, digestive problems, angular cheilitis and anemia [14,18].…”

“…Conventional methods used for the riboflavin determination in drugs, foods, and supplements, such as electrochemistry [18], spectrophotometry [14,20], immunoassay [21], capillary electrophoresis [22] and high-performance liquid chromatography [23,24], require costly solvents, high-cost equipment, and complicated or time-consuming sample preparations [20]. In contrast, the fluorescent method is favored by many, given the method's advantages including on-site testing, faster response, greater simplicity, lower cost and higher sensitivity [14,20].…”

Luminescent metal–organic frameworks for ultrasensitive detection of pesticide and biomarker: In situ imaging and real field application

“…The water‐soluble coenzymes flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) can be produced by RBF 8 . Additionally, RBF has a impart a significant role in various biological processes, including apoptosis, the repair of nucleic acids, and the transport of electrons in the respiratory chain 9,10 . RBF cannot produce in the human body, and thus, we have to take it orally either through food or medicine 11 .…”