Simple Sensitive Spectrophotometric Determination of Vanadium in Biological and Environmental Samples

Abstract

Novel, rapid, highly sensitive and selective spectrophotometric method for the determination of traces of vanadium (V) in environmental and biological samples, pharmaceutical and steel samples was studied. The method is based on oxidation of 2,4- dinitro phenyl hydrazine(2,4-DNPH) by vanadium (V) followed by coupling reaction withN-(1-naphthalene-1-yl)ethane-1,2-diamine-dihydrochloride (NEDA) in acidic medium to give red colored derivative or on oxidation of 4-Amino Pyridine by vanadium (V) followed by coupling reaction with NEDA in basic medium to give pink colored derivative. The red colored derivative having an λmax495 nm which is stable for 8 days and the pink colored derivative with 525 nm is stable for more than 7 days at 350C. Beer's law is obeyed for vanadium (V) in the concentration range of 0.02 - 3.5 μg mL–1(red derivative) and 0.03 – 4.5 μg mL–1(pink derivative) at the wave length of maximum absorption. The optimum reaction conditions and other analytical parameters were investigated to enhance the sensitivity of the present method. The detailed study of various interferences made the method more selective. The proposed method was successfully applied to the analysis of vanadium in natural water samples, plant material, soil samples, synthetic mixtures, pharmaceutical samples and biological samples. The results obtained were agreed with the reported methods at the 95 % confidence level. The performance of proposed method was evaluated in terms of Student'st-test and Variance ratiof-test which indicates the significance of proposed method over reported method.

Catalytic-kinetic spectrophotometric determination of vanadium (V) based on the Celestine blue-bromate-vanadium (V)-citric acid reaction

“…e It was bought from a local market (Murat water, Sivas). Compared with the existing methods [9,18,[20][21]27,[33][34][35][36][37][38], the kinetic-spectrophotometric method developed for V(V) determination in water samples is inexpensive and readily available and allows rapid determination at low operating costs, and shows simplicity, adequate selectivity, comparison of detection limit and linear dynamic range of several kinetic spectrophotometric methods with the proposed method showed in Table 5. In addition, some of the existing kinetic methods need both preheating at high temperatures and long reaction time due to their high activation energies.…”

Spectroscopic Investiqation Complex Formation Of Vanadium Using 2,6-Dithiol-4-Methylphenol And HudrophobAmins

“…Novel, rapid, highly sensitive and selective spectrophotometric method for the determination of traces of V (V) in environmental and biological samples, pharmaceutical and steel samples was studied. The method is based on oxidation of 2,4-dinitro phenyl hydrazine (2,4-DNPH) by V (V) followed by coupling reaction with N-(1 -naphthalene-1-yl)ethane-1,2diamine-dihydrochloride (NEDA) in acidic medium to give red colored derivative or onoxidation of 4 -Amino Pyridine by V (V) followed by coupling reaction with NEDA in basic medium to give pink colored derivative [2]. Se veral kinetic methods based on its catalytic action on the oxidation of organic compounds have been reported in literature [3 -5].…”