Simple sequence repeat markers linked to the everbearing flowering gene in long-day and day-neutral cultivars of the octoploid cultivated strawberry Fragaria × ananassa

Honjo, Masanori; Nunome, Toshiro; Kataoka, Sono; Yano, Takayoshi; Hamano, Megumi; Yamazaki, H.; Yamamoto, Takatsugu; Morishita, Masami; Yui, Susumu

doi:10.1007/s10681-015-1626-6

Cited by 30 publications

(27 citation statements)

References 38 publications

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“…As a consequence, flanking markers tightly linked to FaPFRU are available for MAS, and the candidate genes comprising the locus have been identified. This locus is likely the same as the one described in previous studies (Castro et al ., ; Honjo et al ., ) because all markers developed in our work and in previous studies are located on the same chromosomal region of the LG4 in the diploid reference genome. However, the flanking markers developed in our study are closer to the locus as shown in Figure S1.…”

Section: Discussionmentioning

confidence: 76%

“…Additional Supporting Information may be found online in the supporting information tab for this article: Figure S1 Position on the F. vesca subsp. bracteata pseudochromosome 4 (Tennessen et al, 2014) of the markers linked to perpetual flowering in the octoploid strawberry developed in this study (in bold), in Castro et al (2015) and in Honjo et al (2016) (in italic). Table S1 Description of the 15 and 11 female and male bins of the four linkage groups (LGs) IV in the octoploid Fragaria reference map.…”

Section: Supporting Informationmentioning

confidence: 69%

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Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy

Perrotte

Gaston

Potier

et al. 2016

Plant Biotechnology Journal

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SummaryExtending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry (Fragaria × ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting the perpetual flowering (PF) cultivars. This trait is of considerable interest to growers and to the food industry. Four homoeologous loci controlling a single trait can be expected in such a complex octoploid species. However, we recently showed that the PF trait is under the control of the single dominant FaPFRU locus (J. Exp. Bot., 2013, 64, 1837), making it potentially amenable to marker‐assisted selection (MAS). Here, we report the successful use of a strategy, based on a selective mapping using a reduced sample of individuals, to identify nine markers in close linkage to the FaPFRU allelic variant. Thus, this strategy can be used to fine map the target homoeologous loci in other complex polyploid crop species. Recombinant analysis further enabled us to reduce the locus to a region flanked by two markers, Bx083_206 and Bx215_131, corresponding to a 1.1 Mb region in the diploid F. vesca reference genome. This region comprises 234 genes, including 15 flowering associated genes. Among these, the FLOWERING LOCUS T (FT) is known to be a key activator of flowering. The close association between the PF trait and the FaPFRU flanking markers was validated using an additional segregating population and genetic resources. This study lays the foundation for effective and rapid breeding of PF strawberry cultivars by MAS.

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Section: Discussionmentioning

confidence: 76%

Section: Supporting Informationmentioning

confidence: 69%

Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy

Perrotte

Gaston

Potier

et al. 2016

Plant Biotechnology Journal

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“…A major locus controlling PF was identified, named perpetual flowering and runnering (PFRU) because the PF allele also reduced runnering 51 . Several additional studies in different crossing populations have confirmed PFRU and narrowed the chromosome region 52,[82][83][84][85] . The causal gene is not known, but several candidate genes have been suggested 84 .…”

Section: Flowering and Runneringmentioning

confidence: 90%

A roadmap for research in octoploid strawberry

et al. 2020

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The cultivated strawberry (Fragaria × ananassa) is an allo-octoploid species, originating nearly 300 years ago from wild progenitors from the Americas. Since that time the strawberry has become the most widely cultivated fruit crop in the world, universally appealing due to its sensory qualities and health benefits. The recent publication of the first highquality chromosome-scale octoploid strawberry genome (cv. Camarosa) is enabling rapid advances in genetics, stimulating scientific debate and provoking new research questions. In this forward-looking review we propose avenues of research toward new biological insights and applications to agriculture. Among these are the origins of the genome, characterization of genetic variants, and big data approaches to breeding. Key areas of research in molecular biology will include the control of flowering, fruit development, fruit quality, and plant-pathogen interactions. In order to realize this potential as a global community, investments in genome resources must be continually augmented.Origin and organization of the octoploid strawberry genome

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“…Since the analysis of FaTFL1-RNAi lines has confirmed the function of FaTFL1 as a strong floral repressor in garden strawberry (Koskela et al, 2016), we hypothesize that PFRU is a strong floral activator that bypasses the repressive function of FaTFL1 to promote flowering. Several additional studies in different crossing populations have confirmed PFRU and narrowed down the locus (Castro et al, 2015;Honjo et al, 2016;Perrotte et al, 2016;Salinas et al, 2017;Verma et al, 2017;Hardigan et al, 2018). Causal gene has not been found, but several candidate genes have been suggested (Perrotte et al, 2016;Hardigan et al, 2018).…”

Section: Genetic Control Of Perpetual Flowering In the Garden Strawberrymentioning

confidence: 99%

Control of Flowering and Runnering in Strawberry

Hytönen

Kurokura

2020

Hort. J.

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Strawberry flowering physiology has engaged the interest of researchers for almost a century after the initial reports demonstrating the photoperiodic control of flowering and vegetative reproduction through stolons called runners. Most strawberries possess a seasonal flowering habit with flower initiation occurring under short days in autumn and flowering during the following spring. Also perpetual flowering genotypes are known in diploid woodland strawberry (Fragaria vesca L.) and octoploid garden strawberry (F. × ananassa Duch.), and recent research have shown that this trait has evolved independently in different species. Studies in the perpetual flowering mutant of woodland strawberry led to the identification of TERMINAL FLOWER1 (FvTFL1) as a major floral repressor causing the seasonal flowering habit in this species and demonstrated that recessive mutation in this gene leads to perpetual flowering. This breakthrough opened an avenue for molecular understanding on the control of flowering by different environmental signals. Different loci control perpetual flowering in garden strawberry including one dominant major locus and additional environmentally regulated epistatic loci. The major gene is called Perpetual Flowering Runnering (PFRU) because it also reduces the number of runners. Growth regulator applications initially demonstrated the role of gibberellin in the control of runner formation, and molecular understanding on the role of gibberellin biosynthesis and signaling in this process has started to emerge. Here, we present current understanding and major open questions on the control of flowering and runnering in strawberries. In order to understand the control of flowering in the context of perennial growth cycle, we also discuss current knowledge on the control of dormancy.

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Simple sequence repeat markers linked to the everbearing flowering gene in long-day and day-neutral cultivars of the octoploid cultivated strawberry Fragaria × ananassa

Cited by 30 publications

References 38 publications

Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy

Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy

A roadmap for research in octoploid strawberry

Control of Flowering and Runnering in Strawberry

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