Simple surface modification of poly(ϵ‐caprolactone) to induce its apatite‐forming ability

Oyane, Ayako; Uchida, Masaki; Yokoyama, Yoshiro; Choong, Cleo; Triffitt, James T.; Ito, Atsuo

doi:10.1002/jbm.a.30397

Cited by 140 publications

(139 citation statements)

References 37 publications

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“…Without pretreatment, hardly any apatite formation occurs spontaneously on most synthetic polymers. [40][41][42] This is consistent with our finding that apatite deposited only on COL/PCL/nHA after incubation for 7 days; this can be attributed to the partial dissolution of nHA and subsequent release of calcium ions, which favors formation of apatite, and/or to the exposure of nHA particles on the COL/PCL surface, providing nucleation sites for apatite formation and growth. 43 In addition, an appropriate calcium ion concentration also contributes to the proliferation and differentiation of cells by changing the expression of specific calcium ion channel isoforms.…”

supporting

confidence: 91%

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Wu¹,

Miao²,

Yao³

et al. 2014

IJN

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Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type I collagen/poly(ε-caprolactone)/nanoscale hydroxyapatite (COL/PCL/nHA) with an average fiber diameter of about 377 nm. After a simulated body fluid (SBF) immersion test, the COL/PCL/nHA-SBF scaffold developed a rough surface because of the calcium phosphate deposited on the fibers, suggesting that the presence of nHA promoted the mineralization potential of the scaffold. Energy dispersive X-ray spectroscopy clearly showed the calcium and phosphorus content in the COL/PCL/nHA and COL/PCL/nHA-SBF scaffolds, confirming the findings of nHA and calcium phosphate precipitation on scanning electron micrographs. Water contact analysis revealed that nHA could improve the hydrophilic nature of the COL/PCL/nHA-SBF scaffold. The morphology of periodontal ligament cells cultured on COL/PCL-SBF and COL/PCL/nHA-SBF was evaluated by scanning electron microscopy. The results showed that cells adhered to either type of scaffold and were slightly spindle-shaped in the beginning, then extended gradually with stretched filopodia, indicating an ability to fill the fiber pores. A Cell Counting Kit-8 assay showed that both scaffolds supported cell proliferation. However, real-time quantitative polymerase chain reaction analysis showed that expression of the bone-related markers, alkaline phosphatase and osteocalcin, was upregulated only on the COL/PCL/nHA-SBF scaffold, indicating that this scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells. In this study, COL/PCL/nHA-SBF produced by electrospinning followed by biomimetic mineralization had combined electrospun fibers with nHA in it. This scaffold has good biocompatibility and osteoinductive ability as a result of the characteristics of nHA, so could be innovatively applied to periodontal tissue engineering as a potential scaffold.

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supporting

confidence: 91%

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Wu¹,

Miao²,

Yao³

et al. 2014

IJN

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“…Moreover, the LD-Ap layer enhanced cell attachment to its surface as a result of the biological activity of laminin (Figure 2). Furthermore, using our coating process, an LD-Ap layer was also formed on surface-modified [19][20][21] poly(e-caprolactone) and poly-(L-lactic acid) samples, both of which have an established safety record as bioresorbable biomaterials, 25 and are suitable for tissue engineering and gene therapy applications. It is also well known that apatite phase in the LD-Ap layer has good compatibility with living tissues, especially with bone tissues.…”

mentioning

confidence: 99%

Novel gene-transferring scaffolds having a cell adhesion molecule–DNA–apatite nanocomposite surface

2007

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A low efficiency has long been the most critical problem of conventional gene-transferring systems using calcium phosphates, and this was successfully improved on by using a laminin-DNA-apatite composite (LD-Ap) layer. The genetransferring efficiency of the LD-Ap surface was 1-2 orders of magnitude higher than that of a DNA-calcium phosphate composite surface. This is because laminin enhances cell adhesion and spreading, and this provides regions of high DNA concentration between a cell and the LD-Ap surface. The efficiency of gene transfer of the LD-Ap surface was equivalent to, or even higher than that mediated using a commercial lipid-based transfection reagent applied using the manufacture's recommended optimum conditions. In addition, the gene-transferring efficiency of our system could be controlled by changing the laminin and DNA content in the LD-Ap layer. Moreover, our system is composed of highly safe reagents: apatite, DNA and laminin, all of which are present in the human body. Hence, the LD-Ap surface, which enhances cell attachment on its surface, and mediates a safe, highly efficient and controllable gene transfer, is highly applicable to tissue engineering and gene therapy applications. Gene Therapy (2007) An efficient and safe gene-transferring system is a key technology in tissue engineering and gene therapy applications. 1-12 A gene-transferring system mediated by a particulate DNA-calcium phosphate composite 1-3 is safer than viral 4-6 and lipid-based 7-9 systems, but its transferring efficiency is comparatively low. To enhance the gene-transferring efficiency, a surface-mediated genetransferring system derived from a DNA-apatite composite (D-Ap) layer has been developed. 12 In this system, regions of high DNA concentration are produced locally around cells, and this enhances the gene-transferring efficiency. In this work, the cell adhesion molecule, laminin, 13,14 was immobilized on a D-Ap layer to enhance further the gene-transferring efficiency by strengthening a cell's attachment and facilitating its spreading on a coating's surface. The gene-transferring efficiency was 1-2 orders of magnitude higher on the resulting laminin-DNA-apatite composite (LD-Ap) layer than the gene-transferring efficiency on a D-Ap layer. In this paper, we present an advanced gene-transferring system that has a high efficiency, is safe and has a great potential for use in tissue engineering and gene therapy applications.An LD-Ap layer was coated on a polymer surface in a metastable supersaturated calcium phosphate solution at neutral pH, and ambient pressure and temperature. [15][16][17][18] As controls, an apatite (Ap) layer, a D-Ap layer and a laminin-apatite composite (L-Ap) layer were also coated on the polymer surface. The coating layers were formed by immersing the polymer sample, that had an amorphous calcium phosphate (ACP) layer deposited on its surface, in four different coating solutions: a calcium phosphate solution (CP solution) [15][16][17][18] (for the Ap layer), a CP solution supplemented with DN...

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“…Culture with the MC3T3-E1 cell line showed that the materials were biocompatible and indeed bioactive with the cell activity increased on the coated scaffolds compare to uncoated control scaffolds. Oyane et al [46,47] took plain PCL laid down in 0˚/60˚/120˚ pattern to give a hexagonal scaffold by the group from National University of Singapore, then used either NaOH to produce carboxylate groups on the surface followed by alternate CaCl 2 and K 2 HPO 4 aqueous solutions or oxygen plasma treatment followed by soaking in the same calcium and phosphate salts by dissolved in either water or an ethanol:water mix. Their dipping times were much shorter than the other studies at 10 seconds per dip and only three dips per solution.…”

Section: Ceramic Deposition On To Polymersmentioning

confidence: 99%

Bioactive composites for bone tissue engineering

Tanner

2010

Proc Inst Mech Eng H

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One of the major challenges of bone tissue engineering is the production of a suitable scaffold material. In this review the current composite materials options available are considered covering both the methods of both production and assessing the scaffolds. A range of production routes have been investigated ranging from the use of porogens to produce the porosity through to controlled deposition methods. The testing regimes have included mechanical testing of the materials produced through to in vivo testing of the scaffolds. While the ideal scaffold material has not yet been produced, progress is being made.

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Simple surface modification of poly(ϵ‐caprolactone) to induce its apatite‐forming ability

Cited by 140 publications

References 37 publications

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Novel gene-transferring scaffolds having a cell adhesion molecule–DNA–apatite nanocomposite surface

Bioactive composites for bone tissue engineering

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