Simple UHPLC-DAD Method for the Direct Determination of Donepezil in Cerebrospinal Fluid

Kovatsi, Leda; Tsolaki, Magda; Gkatzima, Olymbia; Petrocheilou, Maria; Samanidou, Victoria

doi:10.1080/10826076.2015.1020165

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The preparation techniques used were mainly solid phase extraction, liquid-liquid extraction and protein precipitation. To the best of our knowledge, a fully validated method for the determination of donepezil in CSF has only been reported once until today [40]. Furthermore, only two methods have been developed for the simultaneous detection of four antidementia drugs (donepezil, galantamne, rivastigmine and memantine) in human plasma by LC-MS and UPLC-MS/MS [41,42].…”

Section: Introductionmentioning

confidence: 99%

A simple and direct HPLC-DAD method for the simultaneous determination of galantamine, donepezil and rivastigmine in cerebrospinal fluid, blood serum and urine

Petrocheilou¹,

Samanidou²,

Kovatsi³

et al. 2017

J Appl Bioanal

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Galantamine (GAL), donepezil (DON) and rivastigmine (RIV) are cholinesterase inhibitors administered to patients who suffer from Alzheimer's disease (AD). We have currently developed and validated an HPLC-DAD method for the determination of GAL, DON, RIV in cerebrospinal fluid (CSF), blood serum and urine. The retention times of the drugs were 1.5, 2.2 and 3.1 min, respectively and the total time of analysis was 5 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to clinical CSF, blood serum and urine samples. The currently developed method is a reliable tool for monitoring CSF, serum and urine galantamine, donepezil and rivastigmine levels in patients under treatment with these drugs.

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Section: Introductionmentioning

confidence: 99%