Simplified and economical 2D IR spectrometer design using a dual acousto-optic modulator

Skoff, David R.; Laaser, Jennifer E.; Mukherjee, Sudipta S.; Middleton, Chris; Zanni, Martin T.

doi:10.1016/j.chemphys.2012.08.019

Cited by 30 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With advances in laser technology, 2D IR spectrometers are now significantly easier to design 27,132,133 and even commercially available. The past decade has also seen integration of theoretical predictions, such as molecular dynamics, into 2D IR studies, and these combined approaches have furthered the scope of 2D IR as a tool in structural biology.…”

Section: Using 2d Ir Of the Amide I Vibration To Determine Static mentioning

confidence: 99%

Watching Proteins Wiggle: Mapping Structures with Two-Dimensional Infrared Spectroscopy

2017

Self Cite

View full text Add to dashboard Cite

Proteins exhibit structural fluctuations over decades of time scales. From the picosecond side chain motions to aggregates that form over the course of minutes, characterizing protein structure over these vast lengths of time is important to understanding their function. In the past 15 years, two-dimensional infrared spectroscopy (2D IR) has been established as a versatile tool that can uniquely probe proteins structures on many time scales. In this review, we present some of the basic principles behind 2D IR and show how they have, and can, impact the field of protein biophysics. We highlight experiments in which 2D IR spectroscopy has provided structural and dynamical data that would be difficult to obtain with more standard structural biology techniques. We also highlight technological developments in 2D IR that continue to expand the scope of scientific problems that can be accessed in the biomedical sciences.

show abstract

Section: Using 2d Ir Of the Amide I Vibration To Determine Static mentioning

confidence: 99%

Watching Proteins Wiggle: Mapping Structures with Two-Dimensional Infrared Spectroscopy

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The output is sent through a periscope, where a 90/10 beam splitter is used to generate vertically offset beams that are sent into a pulse shaper equipped with two Ge-AOMs stacked on top of each other. 36 Although we shape using stacked AOMs, two separate pulse shapers would serve the same purpose. Pulses are modulated in the frequency domain in a 4 f -geometry.…”

Section: Resultsmentioning

confidence: 99%

Spatially Resolved Two-Dimensional Infrared Spectroscopy via Wide-Field Microscopy

et al. 2016

Self Cite

View full text Add to dashboard Cite

We report the first wide-field microscope for measuring two-dimensional infrared (2D IR) spectroscopic images. We concurrently collect more than 16 000 2D IR spectra, made possible by a new focal plane array detector and mid-IR pulse shaping, to generate hyperspectral images with multiple frequency dimensions and diffraction-limited spatial resolution. Both frequency axes of the spectra are collected in the time domain by scanning two pairs of femtosecond pulses using a dual acousto-optic modulator pulse shaper. The technique is demonstrated by imaging a mixture of metal carbonyl absorbed polystyrene beads. The differences in image formation between FTIR and 2D IR microscopy are also explored by imaging a patterned USAF test target. We find that our 2D IR microscope has diffraction-limited spatial resolution and enhanced contrast compared to FTIR microscopy because of the nonlinear scaling of the 2D IR signal to the absorptivity coefficient for the vibrational modes. Images generated using off-diagonal peaks, created from vibrational anharmonicities, improve the molecular discrimination and eliminate noise. Two-dimensional wide-field IR microscopy provides information on vibrational lifetimes, molecular couplings, transition dipole orientations, and many other quantities that can be used for creating image contrast to help disentangle and interpret complex and heterogeneous samples. Such experiments made possible could include the study of amyloid proteins in tissues, protein folding in heterogeneous environments, and structural dynamics in devices employing mid-IR materials.

show abstract

“…Verbesserungender Hardware haben schon jetzt durch erhçhte Stabilitätu nd Automatisierung dieA nwendung vereinfacht. [25,144,145] Fürd ie reinenI R-Pulssequenzen gibt es bereits Messaufbauten, diev on Nichtspezialisten gehandhabt werden kçnnen.E rste kommerziell erhältliche 2D-IR-Spektrometer, welches ogar dieI mplementierung von gemischtenPulssequenzenerlauben, finden ihren Wegauf den Markt. [145,146] Wirs indd aher zuversichtlich, dassd ie Anzahl derW issenschaftler, died ie Mçglichkeitend er hierv orgestellten mehrdimensionalen Experimente nutzenk çnnen (direkto derü berd ie Zusammenarbeit mit einers pezialisierten Arbeitsgruppe), in naherZukunfterheblichwachsenwird.…”

Section: Zusammenfassung Und Ausblickunclassified

Von ultraschnellen Strukturbestimmungen bis zum Steuern von Reaktionen: mehrdimensionale gemischte IR/nicht‐IR‐Schwingungsspektroskopie

Wilderen

Bredenbeck

2015

Angewandte Chemie

View full text Add to dashboard Cite

Ultraschnelle, mehrdimensionale Infrarot‐Spektroskopie ist eine leistungsstarke Methode, um Informationen über molekulare Strukturen und deren Dynamik zu erhalten, die mit linearer Spektroskopie nicht oder nur schwer zugänglich sind. IR‐Pulssequenzen mit resonanten oder nichtresonanten UV‐, Vis‐ oder NIR‐Pulsen erweitern den Einsatzbereich der mehrdimensionalen Schwingungsspektroskopie erheblich und schaffen Möglichkeiten, die weit über die eines reinen IR‐Experimentes hinausgehen. Dazu gehört die oberflächenspezifische 2D‐IR‐Spektroskopie, deren Empfindlichkeit die Untersuchung molekularer Monolagen ermöglicht, die ultraschnelle Strukturbestimmung in Nichtgleichgewichtssystemen, die getriggerte Austauschspektroskopie, die es erlaubt, Edukt‐ und Produktbanden miteinander zu korrelieren, Untersuchung des Zusammenspiels von elektronischen und Kernfreiheitsgraden, die Untersuchung von Wechselwirkungen zwischen Raman‐ und IR‐aktiven Moden, Bildgebung mit chemischem Kontrast, subensembleselektive Photochemie und sogar das Steuern von Photoreaktionen durch selektive IR‐Anregung. Im Folgenden stellen wir nützliche gemischte IR/nicht‐IR‐Pulssequenzen vor, diskutieren deren Eigenheiten und veranschaulichen ihr Anwendungspotenzial.

show abstract

Simplified and economical 2D IR spectrometer design using a dual acousto-optic modulator

Cited by 30 publications

References 34 publications

Watching Proteins Wiggle: Mapping Structures with Two-Dimensional Infrared Spectroscopy

Watching Proteins Wiggle: Mapping Structures with Two-Dimensional Infrared Spectroscopy

Spatially Resolved Two-Dimensional Infrared Spectroscopy via Wide-Field Microscopy

Von ultraschnellen Strukturbestimmungen bis zum Steuern von Reaktionen: mehrdimensionale gemischte IR/nicht‐IR‐Schwingungsspektroskopie

Contact Info

Product

Resources

About