SIMPLIFIED DETERMINATION OF HEMOGLOBIN A1c DIABETIC PATIENTS BY USE OF ELECTROFOCUSING

Jeppsson, J.-O.; Franzen, B.; Gaál, A

doi:10.1515/9783111713625-056

Cited by 19 publications

(10 citation statements)

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“…Haemoglobin Alc (HbAlc) was determined initially and thereafter every four weeks throughout pregnancy. The upper limit of the normal range was 5.5%; the interassay coefficient of variation was 3.5 33 . Each woman performed six daily glucose measurements.…”

Section: Methodsmentioning

confidence: 99%

Fetal size at birth in relation to quality of blood glucose control in pregnancies complicated by pregestational diabetes mellitus

Persson

Hanson²

1996

BJOG

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Objective To determine the relation between maternal levels of blood glucose and glycated haemoglobin (HbAlc) and infant size at birth in pregestational diabetes. Design Longitudinal study from 6 to 14 weeks gestation. Women were treated intensively with insulin, aiming at normoglycaemia but avoiding hypoglycaemia. Blood glucose was determined six times daily, HbAlc every four weeks. Individual mean fasting and postprandial glucose levels were calculated for three‐week periods of gestation. Birthweight > 2 SD or within ±2 SD for gestational age and gender was classified as large (LGA) or appropriate (AGA), respectively. Birthweight ratio was calculated as the ratio of birthweight to normal mean birthweight after correction for gestational age and gender. Participants One hundred and thirteen consecutive pregnant women with pregestational diabetes and their newborn infants. Results Perinatal mortality was nil, the rates of spontaneous preterm delivery (8.9 %) and severe maternal hypoglycaemia (4.4 %) were low. Mothers with LGA infants (26 %) had a significantly higher fasting glucose between weeks 27 and 32 than mothers of AGA infants (P < 0.01). Relative birthweight was significantly and independently associated with pre‐pregnancy bodyweight (r = 0.24, P 005) and fasting glucose at weeks 27 to 29 (r = 0.27, P 0.01) but together could only explain 12.3 % of the variation in birthweight (mult. r = 0.35, P 0.01). HbAlc correlated with glucose levels but was unrelated to birthweight ratio. The fasting glucose level between weeks 30 and 32 was significantly interrelated with the fasting glucose level from each of the six preceding three‐week periods. Conclusion Near normoglycaemia cannot be obtained in all patients, presumably due to intrinsic differences in glucoregulatory ability between individuals. The incidence of LGA infants was unexpectedly high. The modest abnormality in glycaemic control in mothers with LGA infants could only partly explain fetal oversize, suggesting that other factors must be implicated to explain fetal growth acceleration.

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Section: Methodsmentioning

confidence: 99%

Fetal size at birth in relation to quality of blood glucose control in pregnancies complicated by pregestational diabetes mellitus

Persson

Hanson²

1996

BJOG

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“…Blood and urinary glucose was analysed enzymatically using a standard assay (Glox" Pharmacia AB). HbAI, was analysed by the isoelectric focusing technique (19). Using this method the mean value, f S D , in 40 nondiabetic controls was 7.3f0.8%.…”

Section: Biochemical Merhodsmentioning

confidence: 99%

Effect of Metabolic Control and Duration on Exercise ‐ Induced Albuminuria in Diabetic Teen‐agers

et al. 1983

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Nineteen type I diabetic teen-agers without clinical signs of nephropathy with a duration of diabetes varying from 3 to 16.8 years were examined by a standardized exercise test for analysis of urinary excretion of albumin and beta 2-microglobulin. The patients were studied both in poor and improved (but not perfect), metabolic control as defined by HbA1c and blood glucose profiles, and the values were compared to those of 14 age-matched healthy controls. The controls showed no increase in albumin excretion rate during exercise as was found in diabetic patients. The albumin excretion rate during exercise was significantly correlated (p less than 0.05) to systolic blood pressure in the diabetic patients. Blood pressure in the diabetic patients was, however, similar to that of controls both at rest and during exercise. Urinary beta 2-microglobulin did not change during exercise. The urinary albumin excretion during exercise decreased significantly with improved metabolic control in diabetic patients, but the albumin excretion rate was not correlated with either blood or urinary glucose or diuresis during the exercise test. When metabolic control was improved there was a significant correlation between the increase in albumin excretion rate during exercise and the duration of diabetes, indicating that part of the exercise-induced albumin excretion might reflect irreversible morphological changes in the diabetic kidney. This test might therefore have a predictive value for diabetic nephropathy if performed during strict metabolic control.

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“…11 The normal range (mean ± 2 SD) was 5-8.5%. 10 After centrifugation, the cells were hemolysed by addition of 0.01 M potassium cyanide, and the proportion of hemoglobin that had been glycosylated was assessed by isoelectric focusing.…”

Section: Methodsmentioning

confidence: 96%

U.K. Prospective Diabetes Study: II. Reduction in HbA1c with Basal Insulin Supplement, Sulfonylurea, or Biguanide Therapy in Maturity-onset Diabetes

Study¹

1985

Diabetes

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Newly presenting maturity-onset diabetic subjects were put on diet and if, after 3-4 mo, their fasting plasma glucose continued >6 mmol/L, they were randomized to three therapies: (1) continuing diet alone, (2) with additional sulfonylurea, or (3) with additional basal insulin supplement provided by ultralente insulin. Obese patients were also randomized to metformin therapy. The aim was to lower the fasting plasma glucose to <6 mmol/L and the degree to which this reduced the hemoglobin A 1c (HbA 1c ) concentration was studied in 195 patients over 1 yr. Sulfonylurea and insulin similarly reduced (P < 0.001) the fasting plasma glucose from 8.3 ± 1.9 to 6.7 ± 1 . 3 mmol/L (mean ± 1 SD) and 8.6 ± 2.2 to 6.8 ± 1 . 4 mmol/L, respectively. This was accompanied by a significant reduction (P < 0.001) of the HbA 1c to the high normal range, from 9.1 ± 2.1% to 7.8 ± 1.2%, and from 9.1 ± 1.9% to 8.1 ± 1.3%, respectively, both values at 1 yr being significantly (P < 0.05) lower than in patients randomized to diet alone. Patients randomized to diet alone had little change in

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SIMPLIFIED DETERMINATION OF HEMOGLOBIN A1c DIABETIC PATIENTS BY USE OF ELECTROFOCUSING

Cited by 19 publications

References 0 publications

Fetal size at birth in relation to quality of blood glucose control in pregnancies complicated by pregestational diabetes mellitus

Fetal size at birth in relation to quality of blood glucose control in pregnancies complicated by pregestational diabetes mellitus

Effect of Metabolic Control and Duration on Exercise ‐ Induced Albuminuria in Diabetic Teen‐agers

U.K. Prospective Diabetes Study: II. Reduction in HbA1c with Basal Insulin Supplement, Sulfonylurea, or Biguanide Therapy in Maturity-onset Diabetes

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