Simplified Media for the Growth of Haemophilus influenzae from Clinical and Normal Flora Sources

Abstract

~~~~ ~The nutritional requirements of 43 strains of Haemophilus injluenzae isolated from clinical and normal flora sources were investigated. Two defined minimal media were developed by modifying the medium of Herriott et al.( 1 970) : 74 % of the strains could grow on the minimal media and Herriott's medium; the remaining strains could not grow on any of these media.

The Genome-Scale Metabolic Extreme Pathway Structure in Haemophilus influenzae Shows Significant Network Redundancy

“…The in silico strain of H. in-uenzae Schilling, 2000) was allowed inputs that constituted the minimal medium for growth and non-essential amino acid production (Klein & Luginbuhl, 1979;. The components of the minimal medium for the H. in-uenzae model were fructose, glutamate, ammonia, oxygen, arginine, cysteine, heme, NAD, phosphate, panthoenate, putrescine, spermidine, thiamin, and uracil.…”

Sialylation of Lipooligosaccharides Promotes Biofilm Formation by Nontypeable Haemophilus influenzae

“…H. influenzae strains were cultured on brain heart infusion (BHI) media (Difco) supplemented with hemin (ICN Biochemicals) and NAD (Sigma) or on a defined medium (26). NeuAc (20 g/ml; Sigma) was added as indicated below.…”

Assessment of the Metabolic Capabilities of Haemophilus influenzae Rd through a Genome-scale Pathway Analysis

“…di!erent . Most importantly, these determinations in both cases are in accordance with the minimal de"ned media required for the in vitro growth of H. in-uenzae (Klein & Luginbuhl, 1979).…”

“…Biochemical evidence could not be located to determine the ability of H. in-uenzae to metabolize long-chain fatty acids, which would help determine if these genes are ever utilized and if additional genes need to be searched for that are involved in -oxidation. Sets of genes involved in pyrimidine biosynthesis, heme biosynthesis, and thiamin biosynthesis are also not present in any of the extreme pathways, which is supported by the biochemical knowledge that H. in-uenzae requires these metabolites to support its growth (Evans et al, 1974;Klein & Luginbuhl, 1979).…”