Simplified method for the determination of<i>trans</i> monoenes in edible fats by TLC‐GLC

Ulberth, Franz; Henninger, Monika

doi:10.1007/bf02635926

Cited by 31 publications

(20 citation statements)

References 6 publications

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“…In addition, TFA identification has been verified by silver ion thin-layer chromatography. 11 Interassay coefficients of variation in the quality-control pool samples for TFAs were as follows: trans-16:1, 13%; trans-18:1, 8%; and trans-18:2, 11%. Laboratory analyses were conducted by technicians blinded to case and control status.…”

Section: Measurement Of Plasma Phospholipid Fatty Acidsmentioning

confidence: 96%

Plasma Phospholipid Trans Fatty Acids, Fatal Ischemic Heart Disease, and Sudden Cardiac Death in Older Adults

et al. 2006

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Background-Intake of trans fatty acids is associated with increased risk of coronary heart disease. Whether different classes of trans fatty acids show similar associations is unclear. We previously reported an association of sudden cardiac death with red cell membrane trans-18:2 but not trans-18:1 fatty acids. To extend these findings, we investigated the associations of plasma phospholipid trans fatty acids with fatal ischemic heart disease (IHD) and sudden cardiac death. Methods and Results-We conducted a case-control study nested in the Cardiovascular Health Study. We identified 214 cases of fatal IHD (fatal myocardial infarction and coronary heart disease death) between 1992 and 1998. We randomly selected 214 controls, matched to cases on demographics, prevalent cardiovascular disease, and timing of blood draw. Plasma phospholipid fatty acids were assessed in blood samples collected earlier.

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Section: Measurement Of Plasma Phospholipid Fatty Acidsmentioning

confidence: 96%

Plasma Phospholipid Trans Fatty Acids, Fatal Ischemic Heart Disease, and Sudden Cardiac Death in Older Adults

et al. 2006

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“…The oven temperature was programmed from 140 o C at 1.5 o C per minute to 220 o C and held for 15 minutes. For the final determination of the composition of the C18:1 isomers in margarines, the FAME were fractionated by silver-nitrate thin-layer chromatography and further GLC, to avoid the overlap of trans C18:1 (12-trans, 13-trans, 14-trans, 15-trans octadecenoic acid) with cis C18:1 isomer peaks (Ulberth and Henninger, 1992).…”

Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

Fatty acid composition and nutritional relevance of most widely consumed margarines in Spain

Larqué¹,

Garaulet²,

Pérez‐Llamas³

et al. 2003

Grasas y Aceites

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RESUMEN Composición en ácidos grasos de las margarinas de mayor consumo en España y su importancia nutricional.Este estudio examina la composición de ácidos grasos de las margarinas de mayor consumo en España en el año 2000, incluyendo ácidos grasos trans. Todas las margarinas contenían al menos 20% de linoleico, siendo el contenido medio del 38%. Los ácidos grasos saturados (laúrico y mirístico) no sobrepasaron el 4% del total de ácidos grasos. La mayoría de las margarinas contenían menos del 5% de ácidos grasos trans C18:1, aunque la variabilidad era elevada entre las distintas marcas (coeficiente de variación:112%), siendo la mediana del 2.5%; los ácidos grasos trans poliinsaturados C18:2 y C18:3 no representaron más del 1%. Índices nutricionalmente importantes como el cociente áci-dos grasos saturados/insaturados, índices trombogénico y aterogénico, fueron menores de 0,5. Los resultados sugieren que las margarinas españolas muestran un cambio hacia una distribución de ácidos grasos más saludable, aunque debido a la gran variabilidad en su composición, la información del etiquetado debe mejorar para evitar per turbaciones en la tradicional dieta Mediterránea de España. PALABRAS-CLAVE: Ácidos grasos -Ácido linoleico -Cromatografía de gases -Índice de aterogenicidad -Índice de trombogenicidad -Isómeros trans -Margarinas. SUMMARY Fatty acid composition and nutritional relevance of most widely consumed margarines in Spain.This study examines the fatty acid composition of margarines of major consumption in Spain in 2000. All the margarines contained at least 20% of linoleic acid, the average content of this fatty acid being 38%. Saturated fatty acids (lauric and miristic acids) did not exceed 4% of the total fatty acid content. Most of the margarines analyzed contained less than 5% trans C18:1, although this cont ent varied greatly among margarines (coefficient of variation: 112%) being median value 2.5%; polyunsaturated trans C18:2 and trans C18:3 did not represent m o r e t h a n 1 % . N u t r i t i o n a l l y i m p o r t a n t r a t i o s l i k e saturated/unsaturated fatty acids, thrombogenicity and atherogenicity indexes were lower than 0.5. The findings suggest that Spanish margarines have moved to becoming products with a potentially healthier distribution of fatty acids. Even so, the great variability shown in fatty acid composition of margarines and poor labeling, highlight the importance of greater consumer information to avoid upsetting the traditional Mediterranean diet of Spain.

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“…The fatty acid composition of food is usually determined using gas-liquid chromatography of the corresponding fatty acid methyl esters (FAMEs) (Baggio et al, 2005;Bondia-Pons et al, 2004;Chen et al, 1999;Ratnayake, 1995;Ulberth & Henninger;1992). Usually, the FAMEs can be conveniently prepared by heating lipids with a large excess of either acidcatalysed or base-catalysed reagents.…”

Section: Analytical Methods For Trans Fatty Acid Determinationmentioning

confidence: 99%

Trans Fatty Acids and Human Health

Filip¹,

Vidrih²

2012

The Cardiovascular System - Physiology, Diagnostics and Clinical Implications

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Simplified method for the determination oftrans monoenes in edible fats by TLC‐GLC

Cited by 31 publications

References 6 publications

Plasma Phospholipid Trans Fatty Acids, Fatal Ischemic Heart Disease, and Sudden Cardiac Death in Older Adults

Plasma Phospholipid Trans Fatty Acids, Fatal Ischemic Heart Disease, and Sudden Cardiac Death in Older Adults

Fatty acid composition and nutritional relevance of most widely consumed margarines in Spain

Trans Fatty Acids and Human Health

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