Simplified Rapid Technic for the Extraction and Determination of Serum Cholesterol without Saponification

Carr, Julius J.; Drekter, I. J.

doi:10.1093/clinchem/2.5.353

Cited by 172 publications

(21 citation statements)

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“…Measurement of cholesterol and phospholipids was done using established methods (Carr & Dreckter, 1956;Bartlett, 1959). Bile salts were measured using high performance liquid chromatography (HPLC), as previously described .…”

Section: Biochemical Methodsmentioning

confidence: 99%

Effects of Histamine and Histamine Antagonists on Hepatic Bile Flow in the Conscious Sheep

Lee

1984

Clin Exp Pharma Physio

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The effects of histamine and histamine antagonists on bile flow have been studied using the cholecystectomized conscious sheep. Histamine stimulated bile flow in a dose-dependent manner (D50 = 15.7 micrograms/h per kg). Free water and HCO3-output were increased after intravenous histamine infusion. With histamine at 120 mg/h per kg, there was a 41.0 (s.d. = 4.6)% and 38.7 (s.d. = 4.2)% (n = 6) increase in bile volume and HCO3-output respectively. Bile salt concentration was decreased after intravenous histamine, but net bile salt secretory rate remained unchanged. There was no change observed in the molar ratios of bile salts: phospholipids: cholesterol. Total lipids were decreased from 2.2 (s.d. = 0.3) to 1.60 (s.d. = 0.4) g/dl (n = 6). The corrected lithogenic index did not change significantly. 14C-Erythritol clearance also increased during histamine infusion in a dose-dependent manner but did not correspond to increments in bile flow. The histamine H1-receptor antagonist, diphenhydramine 0.05-1.0 mg/h per kg did not alter histamine stimulated bile flow. The H2-receptor antagonists, cimetidine (0.5-4 mg/h per kg) and ranitidine (0.05-0.5 mg/h per kg) progressively reversed the histamine-induced choleresis. Maximum inhibitory effect was attained at 2.0 and 0.25 mg/h per kg for cimetidine and ranitidine, respectively. At these levels, variation of intravenous infusion of histamine did not result in competitive displacement of the inhibitory response by either cimetidine or ranitidine. Moreover, concomitant infusion of diphenhydramine at 1.0 mg/h per kg potentiated the inhibitory effect of cimetidine or ranitidine on histamine-induced choleresis. Histamine increases the bile salt independent component of hepatic bile flow.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Biochemical Methodsmentioning

confidence: 99%

Effects of Histamine and Histamine Antagonists on Hepatic Bile Flow in the Conscious Sheep

Lee

1984

Clin Exp Pharma Physio

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“…The lipid extract was collected in a weighed beaker and dried to constant weight at 60" for total-lipid determination. The lipid extract was redissoIved in the above solvent or cells were saponified and the sterols extracted with petroleum ether for estimation of cholesterol by the Lieberman-Burchard procedure (4). Lipid analyses were done at least 4 times and average results are presented.…”

Section: Methodsmentioning

confidence: 99%

Lipid and Sterol Content of Some Protozoa*

Aaronson

Baker

1961

The Journal of Protozoology

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Total lipids of several protozoa were determined and found higher than in most other microorganisms. The lipid content in a phytoflagellate, Ochromonas malhamensis, could be increased by excess nicotinic acid or deficiency in nitrogen supply. Cell multiplication and lipid synthesis were unaffected by inhibitors of vitamin synthesis such as penicillamine, pan‐toyltaurine, or pyridine‐3‐sulfonic acid, up to 100 mg%. The phytoflagellates, O. danica, O. malhamensis, Euglena gracilis, and the photosynthetic bacterium Chlorobium limkola contained large quantities of non‐saponifiable lipids: ergesterol was identified as a major component of this lipid fraction. Sterols were not found in the bacteria, Streptococcus faecalis, Rhodopseudomonas palustris; the algae. Cyanidium calidarnm. Anacystis nidulans, and Porphyridium cruentum; and the protozoan Tetrahymena pyriformis.

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“…After scanning of the electropherogram in a Photovolt curve resolver with integrator at 650 nm and determination of total serum triglyceride (Laurel1 1966), cholesterol (Carr & Drekter 1956) and phospholipids (Walaas et al 1971), the absolute and relative amounts of the different lipoprotein classes were estimated in accordance with the studies by Dyerberg & HjGrne (1970). In one of the cases the individual phospholipids were determined by thin layer chromatography as previously reported (Walaas et al 1971).…”

Section: Methodsmentioning

confidence: 92%