Simplified Serum Phosphorus Analyses by Continuous-Flow Ultraviolet Spectrophotometry

Abstract

Serum phosphorus can be measured by continuous-flow uv spectrophotometry without reduction of the phosphomolybdate complex. The dilute sample is dialyzed into dilute (1 ml/100 ml) sulfuric acid, then mixed with an ammonium molybdate— sulfuric acid—"Tween 80" solution. The absorbance of the sample peaks is measured at 340 nm with a linear-absorbance spectrophotometer. Peak heights are directly proportional to concentration, because logarithmic conversion is performed within the spectrophotometer. The method obeys Beer’s law up to 10 mg of P per deciliter, and results correlate closely with those for standard methods based on reduced phosphomolybdate blue. The miniature manifold uses a 12-in. dialyzer. The sampling rate can be 60 to 120 samples per hour when a Gilford one-piece debubbler flow cell is used.

Serum Chemistry of the Minke Whale From the Northeastern Atlantic

“…Value of ␣ was set as P Յ 0.05. Bergmeyer et al, 1978Keiding et al, 1974Keiding et al, 1974Szasz et al, 1976Keiding et al, 1974 Szasz, 1976 GLDH a Amylase Lipase Protein (total) Albumin Globulin DGKC d CNP-G3 e kinetic UV turbidometric biuret bromcresol green (total protein-albumin) Bergmeyer et al, 1970Winn-Deen et al, 1988Fossati et al, 1992Skeggs and Hochstrasser, 1964Doumas, 1971 Urea Creatinine Uric acid Bile acids urease alkaline picrate, kinetic uricase enzymatic, colorimetric Tiffany et al, 1972Rossignol et al, 1984Fossati et al, 1980Mashige et al, 1976 Bilirubin (total) Cholesterol Triglycerides Non-esterified fatty acids blanked diazo enzymatic, colorimetric enzymatic, colorimetric enzymatic, colorimetric Novros et al, 1979Allain, 1974Fossati and Prencipe, 1982 Wako NEFA C test kit Glucose Phosphorus Calcium Magnesium hexokinase phosphomolybdate cresolphtalein enzymatic Slein et al, 1950Amador and Urban, 1972Gitelman, 1967Fossati et al, 1989…”

Comparison of Lacrimal and Salivary Gland Involvement in Sjögren's Syndrome

“…Inorganic phosphate was measured at 340 and 383 nm after addition of molybdate and reduction with bisulfite in the presence of p-methylaminephenolsulfate. 15,16…”

Sperm Morphology and Natural Biomolecules from Marine Snail Telescopium telescopium: a Phylogenetic Perspective

“…Serum glutamic pyruvic transaminase (SGPT), Serum glutamic oxaloacetic transaminase (SGOT) and gamma gluteryl transferase (GGT) was estimated according to IFCC methods (1986). Determination of quantity of chloride (Schoenfeld & Leweller, 1964), Albumin (Doumas et al, 1972), HDL-Cholesterol (Burstein et al, 1970), Triglycerides (Schettler & Nussel, 1975), Creatinine (Fabing & Ertingshausen, 1971), Glucose (Tietz, 1982), Iron (Siedel et al, 1984) Magnesium (Whitmore & Evans, 1964), Copper & Zinc (Agarwal & Henkin, 1985), Lithium (Vonknoring et al, 1976), Total protein (Doumas et al, 1975), Urea (Fawcett & Scott, 1960), Inorganic phosphorus (Amador & Urban, 1977) and Bicarbonate (Tietz, 1976) were also estimated by kits (Bayer Diagnostics India Ltd. Gujrat, India; Erba Diagnostics Mannheim GmbH, Germany; Reckon Diagnostic P. Ltd., 3/ 7 industrial estate, Baroda , India; Merck Limited, Worli, Mumbai, India; Invitro Biotech Ltd., Hyderabad , India; Crest Biosystems, Goa, India); Estimation of hormonal levels, if any, were also determined for Testosterone (Ismail et al, 1972), Insulin (Eastham, 1985), thyroid stimulating hormone (TSH) (Spencer, 1995), Estradiol and Progesterone (Tietz, 1986) (Equiper via G Ferrari, Italy, Monobind Inc. Lake forest, CA, USA both for insulin and TSH and DRG Instruments GmbH, Germany for estradiol and progesterone respectively).…”