Simplified Turbidimetric Determination of Apolipoproteins A-I, A-II and B Using a Microtitre Method

Sandkamp, M.; Tambyrajah, B. M.; Schriewer, H; Assmann, Gerd

doi:10.1515/cclm.1988.26.11.685

Cited by 20 publications

(11 citation statements)

References 5 publications

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“…HDL cholesterol was analyzed after precipitation with phosphotungstic acid/MgCI2. Apo A-I was quantified by turbidimetry as described (9). The rate of cholesterol esterification in plasma was determined by two methods.…”

Section: Methodsmentioning

confidence: 99%

A frameshift mutation in the human apolipoprotein A-I gene causes high density lipoprotein deficiency, partial lecithin: cholesterol-acyltransferase deficiency, and corneal opacities.

Funke¹,

Eckardstein²,

Pritchard³

et al. 1991

J. Clin. Invest.

122

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Section: Methodsmentioning

confidence: 99%

A frameshift mutation in the human apolipoprotein A-I gene causes high density lipoprotein deficiency, partial lecithin: cholesterol-acyltransferase deficiency, and corneal opacities.

Funke¹,

Eckardstein²,

Pritchard³

et al. 1991

J. Clin. Invest.

122

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“…31 Apolipoproteins were measured by Dr A. von Eckardstein at the Institut fur Klinische Chemie und Laboratoriumsmedizin, Munster, FRG, by using a turbidimetric method on microtiter plates as described. 32 Lipoprotein and apolipoprotein values obtained at the two sampling days at the end of each dietary period were averaged for data analyses.…”

Section: Blood Sampling and Analysismentioning

confidence: 99%

Impact of myristic acid versus palmitic acid on serum lipid and lipoprotein levels in healthy women and men.

Zock

Vries

Katan

1994

Arterioscler Thromb

247

146

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The cholesterol-raising effect of dietary saturated fatty acids is largely accounted for by lauric, myristic, and palmitic acids. Dairy fat is a major source of myristic acid, and palm oil is especially rich in palmitic acid. Myristic acid is suspected of being much more cholesterolemic than palmitic acid, but direct comparisons have been lacking. We therefore fed 36 women and 23 men three diets that differed from each other in palmitic, oleic, and myristic acid content by about 10% of total energy. We used palm oil, high-oleic acid sunflower oil, and a specially produced high-myristic acid fat to achieve these differences. Each diet was consumed for 3 weeks in random order. Mean serum cholesterol was 4.53 mmol/L on the high-oleic acid diet, 4.96 mmol/L on the palmitic acid diet, and 5.19 mmol/L on the myristic acid diet (/'<.0001 for all comparisons). Myristic acid raised low-density upoprotein (LDL) cholesterol by 0.11 mmol/L, high-density R educing saturated fat (and cholesterol) intake is the therapy of choice in the treatment of moderate hypercholesterolemia and an important adjunct to drug therapy in the treatment of severe hypercholesterolemia.1 Restriction of saturated fatty acids in the diet is more effective than limiting total fat consumption.2 However, saturates with different chain lengths may differ in their cholesterol-raising effect. Lauric acid (C12:0), myristic acid (C14:0), and palmitic acid (C16:0) are thought to increase cholesterol levels, whereas stearic acid (C18:0) has little or no effect.3 7 In experimental animals, saturated fatty acids raise plasma cholesterol through downregulation of low-density lipoprotein (LDL) receptor activity and subsequent accumulation of LDL cholesterol (LDL-C) in plasma and increased LDL production from its precursor, very-lowdensity lipoprotein (VLDL). 8 -9 These effects are seen with lauric, myristic, and palmitic acids but not with medium-chain saturates (C6:0, C8:0, and C10:0) or stearic acid. 10 In humans lauric acid is less potent than palmitic acid in raising total cholesterol and LDL-(34,11,12 Myristic acid and palmitic acid together make up about 25% to 30% of the fat in Western diets (D. Kromhout, unpublished data, 1988), but their relative cholesterol-raising potencies have not been clearly defined in humans. Meta-analyses of dietary trials that employed commercially available fats indicate that myristic acid might be four to six times more cholesterReceived October 29, 1993; revision accepted January 3, 1994. From the Department of Human Nutrition, Wageningen Agricultural University, Wageningen, the Netherlands.Correspondence to Prof Dr M.B. Katan, Department of Human Nutrition, Wageningen Agricultural University, Bomenweg 2, 6703 HD Wageningen, the Netherlands. lipoprotein (HDL) cholesterol by 0.12 mmol/L, and apolipoprotein (apo) A-I by 7.2 mg/dL relative to palmitic acid; increases relative to oleic acid were 0.50 mmol/L for LDL cholesterol, 0.15 mmol/L for HDL cholesterol, 6.0 mg/dL for apoB, and 8.9 mg/dL for apoA-I (P<.01 for all c...

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“…Concentrations of apo A-I, apo A-II, and apo B were determined with a modified commercially available turbidimetric assay (Boehringer Mannheim). 47 Apo A-IV was quantified by electroimmunodiffusion and a rabbit anti-human apo A-IV antiserum. 48 The serum concentration of HDL containing apo A-I but not apo A-II (Lp A-I) was determined with a commercially available electroimmunodiffusion assay (Sebia).…”

Section: Measurements Of Lipids Lipoproteins and Apolipoproteinsmentioning

confidence: 99%

Sex-specific effects of the glutamine/histidine polymorphism in apo A-IV on HDL metabolism.

Eckardstein

Funke

Chirazi

et al. 1994

Arterioscler Thromb

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Simplified Turbidimetric Determination of Apolipoproteins A-I, A-II and B Using a Microtitre Method

Cited by 20 publications

References 5 publications

A frameshift mutation in the human apolipoprotein A-I gene causes high density lipoprotein deficiency, partial lecithin: cholesterol-acyltransferase deficiency, and corneal opacities.

A frameshift mutation in the human apolipoprotein A-I gene causes high density lipoprotein deficiency, partial lecithin: cholesterol-acyltransferase deficiency, and corneal opacities.

Impact of myristic acid versus palmitic acid on serum lipid and lipoprotein levels in healthy women and men.

Sex-specific effects of the glutamine/histidine polymorphism in apo A-IV on HDL metabolism.

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